Similar studies for GEO DataSets (Select 200008564) - GEO DataSets

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Items: 20

Select item 2000085641.

Analysis of Aire effects on individual mice of different genetic backgrounds

(Submitter supplied) We compared gene expression profiles of aire-deficient and wild-type littermate thymic medullary epithelial cells. This was done in order to determine whether Aire's effects differed among strains, and also among individuals of the same strain. Keywords: genetic modification

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1261
20 Samples

Download data: CEL

Series

Accession:: GSE8564

ID:: 200008564

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2000703262.

Expression data from cortical thymic epithelial cells ectopically expressing Aire

(Submitter supplied) Aire in medullary thymic epithelial cells plays an essential role in the negative selection through expression of broad arrays of tissue-restricted antigens. We asked whether Aire could also activate the expression of tissue-restricted antigens in cortical thymic epithelial cells.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6246
2 Samples

Download data: CEL

Series

Accession:: GSE70326

ID:: 200070326

Select item 2000000853.

wild type and aire -/- murine meduallary thymic epithelial cells

(Submitter supplied) Mice used were B6/129 F2's, 3-5 weeks of age, either wild type or with both copies of the autoimmune regulator gene (aire, GenBank #AF079536) disrupted. Thymi from five of these mice of both sexes were removed and pooled. After collagenase/dispase digestion, density gradient fractionation, and fluorescent antibody staining, cells with the phenotype CD45-, G8.8+, CDR1int and B7.1hi were FACS-sorted and total RNA was made from them. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Dataset:: GDS167
Platform:: GPL81
6 Samples

Download data: CEL

Series

Accession:: GSE85

ID:: 200000085

Select item 1674.

Autoimmune disease mechanisms

Examination of medullary thymic epithelial cells from aire-deficient mice. aire is autoimmune regulator gene. Aim to understand autoimmune disease mechanisms, esp. autoimmune polyendocrinopathy candidiasis ectodermal dystrophy (APECED).

Organism:: Mus musculus

Type:: Expression profiling by array, count, 2 strain sets

Platform:: GPL81
Series:: GSE85
6 Samples

Download data: CEL

DataSet

Accession:: GDS167

ID:: 167

Select item 2000656175.

Differential features of Aire-induced and Aire-independent promiscuous gene expression in thymic epithelial cells

(Submitter supplied) Promiscuous gene expression (pGE) of numerous self-antigens in thymic epithelial cells (TEC) enables the elimination of self-reactive T cells. The autoimmune regulator (Aire) is the only known molecular determinant driving pGE in the thymus but the existence of Aire-independent mechanisms has been inferred. Here, we analyzed the poly(A)+ transcriptome of TEC populations by RNA-sequencing (RNA-seq) in order to reveal differential features of Aire-induced vs. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL13112
18 Samples

Download data: TXT

Series

Accession:: GSE65617

ID:: 200065617

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2000707986.

Single cell transcriptomic analysis of thymic epithelial cells

(Submitter supplied) Single cell transcriptomic analysis of wildtype and AireKO thymic epithelial cells

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL17021
200 Samples

Download data: CSV, TXT

Series

Accession:: GSE70798

ID:: 200070798

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2000614447.

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Jmjd6-/- Thymic stromal Transcriptomes

(Submitter supplied) The goals of this study are to comprehensively identify genes controlled by Jmjd6 in the thymic stroma, and to identify a novel alternative splicing mechanism. Methods: Samples were WT and Jmjd6-/- fetal thymus organ culture with (2 samples for each category) or without (1 sample for each category) RANKL stimulation for 4 days under 2-DG. One µg of total RNA was used for library construction with TruSeq RNA Sample Prep Kit v2. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL13112
6 Samples

Download data: TXT, XLSX

Series

Accession:: GSE61444

ID:: 200061444

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001553318.

Transcriptional regulation by Aire in medullary thymic epithelial cells revisited

(Submitter supplied) The deficiency of Aire, a transcriptional regulator whose defect results in the development of autoimmunity, is associated with reduced expression of tissue-restricted self-Ags (TRAs) in medullary thymic epithelial cells (mTECs). Although the mechanisms underlying Aire-dependent expression of TRAs need to be explored, the physical identification of the target(s) of Aire has been hampered by the low and promiscuous expression of TRAs. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:: GPL13112 GPL17021
14 Samples

Download data: BW, MTX, TSV

Series

Accession:: GSE155331

ID:: 200155331

PubMed Similar studies SRA Run Selector

Select item 2000247349.

Gene Expression Profile of Medullary Epithelial Cells isolated from thymus of Bone Marrow (BM) reconstituted RAG1 null (control) and SCID NOD mouse

(Submitter supplied) We identified DNAPK as one of the major proteins that physically interact with Autoimmune regulator (Aire). To establish physiological significance of DNAPK in Aire-driven expression of PTA genes in MECs, we utilized BM-reconstituted SCID mice (which express non functional DNAPK in their MECs) and RAG1 null mouse as a control. MECs from these mutated/reconstituted mice were sorted by Flow cytomety and their expression profile was analyzed using Affymetrix chips. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6246
7 Samples

Download data: CEL

Series

Accession:: GSE24734

ID:: 200024734

Select item 20002473310.

Gene Expression Profile of 293T and 293T-Aire-expressing cells

(Submitter supplied) To analyze the impact of Aire on gene expression profile in a model cell line, we used 293T cells and transfected them either with an Aire expression plasmid pCMV-Aire (where mAire is driven by CMV promoter) or with a control plasmid pCMV2B. Total RNA was extracted 48 hours post transfection, processed and used for gene expression profiling by Affymetrix. The data demonstrate that Aire has a very broad impact, effecting (upregulating and downregulating) hundreds of differents genes, however these genes differ dramatically from its targets in medullary epithelial cells. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL570
5 Samples

Download data: CEL

Series

Accession:: GSE24733

ID:: 200024733

Select item 20009936811.

Expression data from B cells expressing human AIRE

(Submitter supplied) Aire in medullary thymic epithelial cells plays an essential role in the establishment of self-tolerance through expression of broad arrays of tissue-restricted antigens. We asked what would happen if human AIRE is additively expressed in antigen-presenting cells in the thymus and periphery.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1261
2 Samples

Download data: CEL

Series

Accession:: GSE99368

ID:: 200099368

Select item 20003387812.

Expression data from WT and Aire KO thymic medullary epithelial cells (MECs)

(Submitter supplied) The aim of this study is to analyze the transcriptional effects of Aire deficiency in the thymus, using the Affymetrix MoGene platform to analyze variation in exon usage

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6246
6 Samples

Download data: CEL

Series

Accession:: GSE33878

ID:: 200033878

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20006910513.

Expression data from WT and Fezf2 KO mTECs

(Submitter supplied) Fezf2 is highly and specifically expressed in mTECs in mouse thymus and Fezf2 deficiency (Fezf2 KO) in the thymus leads to autoimmunity. However, it is unclear how Fezf2 contributes to thymic gene expression. We collected WT and Fezf2 KO mTECs by FACS, and performed microarrays to determine genes regulated by Fezf2.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1261
10 Samples

Download data: CEL

Series

Accession:: GSE69105

ID:: 200069105

Select item 20006640214.

Gene expression profiling of perinatal or adult medullary thymic epithelial cells (MECs)

(Submitter supplied) A comparative analysis of gene expression of perinate or adult Aire-GFP+ and GFP- MECs in WT or Aire-KO thymus

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6246
17 Samples

Download data: CEL

Series

Accession:: GSE66402

ID:: 200066402

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20006633215.

Gene expression profiling of perinatal or adult regulatory T cells (Tregs)

(Submitter supplied) A comparative analysis of gene expression of 3 different experiments; 1. Perinate or adult-tagged GFP+YFP+ and bulk GFP+YFP- Tregs, 2. FL or BM-derived Tregs 3. Perinate or adult thymic Tregs.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6246
24 Samples

Download data: CEL

Series

Accession:: GSE66332

ID:: 200066332

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20006343216.

Genome-wide analysis of the TEC-specific Hipk2 gene knockout in mouse medullary thymic epithelial cells (mTECs)

(Submitter supplied) Analysis of the role of Hipk2 in regulating gene expression in medullary thymic epithelial cells. The whole genome gene signatures of purified mTEC subsets (CD80 low, CD80 high) from TEC-specific Hipk2 knockout mice were compared to mating wildtype control mice (floxed, Cre-). Results provide the up- or down-regulated genes, affected by the Hipk2 gene knockout.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL17543
8 Samples

Download data: IDAT, TXT

Series

Accession:: GSE63432

ID:: 200063432

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20000258517.

promiscuous gene expression in the mouse thymus

(Submitter supplied) Gene expression in different thymic stromal cells and subsets thereof was analyzed in 6-12 week old wild type (C57BL/6) and Aire knock-out (mixed background) mice. Thymic stromal cells were purified by sequential enzymatic digestion (collagenase, collagenase/dispase and trypsin) followed by gradient centrifugation and FACS sorting. Sort criteria were as follows: dendritic cells (CD11c+, F4/80 -), macrophages (F4/80+, CD11c-), cTECs (CD45–/lo, CDR1/Ly51+, Ep-CAM+) and mTECs (CD45–/lo, CDR1/Ly51–, Ep-CAM+). more...

Organism:: Mus musculus

Type:: Expression profiling by array

Datasets:: GDS1655 GDS2274
Platform:: GPL81
16 Samples

Download data: CEL

Series

Accession:: GSE2585

ID:: 200002585

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 227418.

Transcription factor Aire deficiency effect on medullary thymic epithelial cells

Analysis of transcription factor Aire deficient medullary thymic epithelial cells with low or high CD80 expression. Aberrent expression of tissue-restricted antigens, called promiscuous gene expression (PGE), is a property of the thymic stroma. Results provide insight into the role of Aire in PGE.

Organism:: Mus musculus

Type:: Expression profiling by array, count, 2 cell type, 2 genotype/variation sets

Platform:: GPL81
Series:: GSE2585
8 Samples

Download data: CEL

DataSet

Accession:: GDS2274

ID:: 2274

PubMed Full text in PMC Similar studies GEO Profiles Analyze DataSet

Select item 165519.

Medullary thymic epithelial cells

Comparison of medullary thymic epithelial cells (TECs) to cortical TECs, dendritic cells, and macrophages. Aberrent expression of tissue-restricted antigens, called promiscuous gene expression (PGE), is a property of the thymic stroma. Results provide insight into the scope of PGE in the thymus.

Organism:: Mus musculus

Type:: Expression profiling by array, count, 4 cell type sets

Platform:: GPL81
Series:: GSE2585
8 Samples

Download data: CEL

DataSet

Accession:: GDS1655

ID:: 1655

PubMed Full text in PMC Similar studies GEO Profiles Analyze DataSet

Select item 20001436520.

Expression data from WT and Aire deficient mTECs

(Submitter supplied) Aire is an important transcription regulator that mediates a role in central tolerance via promoting the promiscuous expression of tissue-specific antigens in the thymus. Although several mouse models of Aire-deficiency have been described, none has analysed the phenotype induced by a mutation that emulates the common 13bp deletion in human APECED by disrupting the first PHD domain in exon 8. Aire-deficient mice with a corresponding mutation showed some disturbance of the medullary epithelial compartment, but at the phenotypic level their T cell compartment appeared relatively normal in the thymus and periphery. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1261
5 Samples

Download data: CEL

Series

Accession:: GSE14365

ID:: 200014365

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