GEO DataSets

(Submitter supplied) In intact animals, time of drug administration may be an important factor influencing drug response. Our general goal seeks to incorporate circadian time into the study of corticosteroid regulated gene expression. This study is designed to examine fluctuations in gene expression in skeletal muscle within the 24 hour circadian cycle in normal animals. Circadian time is relevant to designing optimal corticosteroid dosing regimens. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS3083

Platform:

GPL341

54 Samples

Download data: CEL

Analysis of gastrocnemius muscles of Wistar animals during a 24-hour period, from zeitgeber time (ZT) 0.25 to ZT 23.75. Results provide insight into baseline patterns of gene expression in normal skeletal muscle within the 24 hour circadian cycle.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 18 time sets

Platform:

GPL341

Series:

GSE8989

54 Samples

Download data: CEL

(Submitter supplied) Circadian rhythms are oscillations with a periodicity of 24 hours that are controlled by an endogenous clock and are observed in virtually all aspects of mammalian function from expression of genes to complex physiological processes. The master clock is present in the suprachiasmatic nucleus (SCN) in the anterior part of the hypothalamus and controls peripheral clocks present in other parts of the body. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

52 Samples

Download data: CEL, CHP

(Submitter supplied) Circadian rhythms are central to optimal physiological functioning and their interruption contributes to the development of several chronic diseases. Alcohol (EtOH) intoxication disrupts circadian rhythms within liver, brain, and intestines, but it is unknown whether alcohol also disrupts components of the core clock in skeletal muscle. Female C57BL/6Hsd mice were randomized to receive either saline (control) or alcohol (EtOH) (5g/kg) via intraperitoneal injection at the start of the dark cycle (ZT12), and gastrocnemius was collected every 4hr from Control and EtOH treated mice for the next 48hr following isoflurane anesthetization. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

10 Samples

Download data: XLSX

(Submitter supplied) Circadian rhythms are oscillations with a periodicity of 24 hours that are controlled by an endogenous clock and are observed in virtually all aspects of mammalian function from expression of genes to complex physiological processes. The master clock is present in the suprachiasmatic nucleus (SCN) in the anterior part of the hypothalamus and controls peripheral clocks present in other parts of the body . more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

54 Samples

Download data: CEL, CHP

(Submitter supplied) The aim of this project is to identify distinct temporal patterns of RNA expression in the skeletal muscle of rats following a bolus dose of the corticosteroid methylprednisolone. 51 RG_U34A chips were used over 17 time points. Keywords: other

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS256

Platform:

GPL85

51 Samples

Download data: CEL

Temporal analysis of skeletal muscle response to corticosteroid methylprednisolone. Wistar rats injected with methylprednisolone and skeletal muscle analyzed at various time points up to 72 hours.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 17 time sets

Platform:

GPL85

Series:

GSE490

51 Samples

Download data: CEL

(Submitter supplied) In intact animals, time of drug administration may be an important factor influencing drug response. Our general goal seeks to incorporate circadian time into the study of corticosteroid regulated gene expression. Since levels of endogenous steroid exhibit circadian fluctuations, it is our hypothesis that the expression of genes controlled by corticosteroids either directly or indirectly, will also exhibit a circadian pattern in normal animals. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS3084

Platform:

GPL341

54 Samples

Download data: CEL

Analysis of livers of Wistar animals during a 24-hour period, from zeitgeber time (ZT) 0.25 to ZT 23.75. Results provide insight into baseline patterns of gene expression in normal liver within the 24 hour circadian cycle.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 18 time sets

Platform:

GPL341

Series:

GSE8988

54 Samples

Download data: CEL

(Submitter supplied) Four healthy human volunteers underwent an acute bout of resistance exercise with the right leg at 2 pm. Biopsies were removed from the Vastus Lateralis muscle 6 h (8 pm) and 18 h (8 am) after exericise Keywords = Human skeletal muscle Keywords = resistance exerise Keywords = diurnal Keywords = circadian Keywords: time-course

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS894

Platform:

GPL8300

15 Samples

Download data: CEL

Analysis of effects of resistance exercise (RE) and time of day in skeletal muscle. Biopsies of male vastus lateralis muscle obtained 6 and 18 hours after acute RE, or no RE. Results suggest that RE may directly modulate circadian rhythms in skeletal muscle.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 stress, 2 time sets

Platform:

GPL8300

Series:

GSE1832

15 Samples

Download data: CEL

(Submitter supplied) Synthetic glucocorticoids are used therapeutically for a variety of conditions. Both the efficacy and toxicity of corticosteroids arise from their pharmacologically exaggerated effects on target and non-target tissues. For example, beneficial effects deriving from inhibition of the immune system are accompanied by toxic side effects that include hyperglycemia, dyslipidaemia, muscle wasting, fatty liver, and an increased risk of atherosclerosis. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS2688

Platform:

GPL341

44 Samples

Download data: CEL

Analysis of skeletal muscles of adrenalectomized animals given methylprednisolone (MPL) at a constant rate of infusion for up to 7 days. Results compared to a time series experiment using a single dose of MPL. Results provide insight into the identity of genes regulated by MPL in both regimens.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 11 time sets

Platform:

GPL341

Series:

GSE5101

44 Samples

Download data: CEL

(Submitter supplied) To determine whether immortalized cells derived from the rat SCN (SCN 2.2) retain intrinsic rhythm-generating properties characteristic of the SCN, oscillatory properties of the SCN2.2 transcriptome were analyzed and compared to those found in the rat SCN in vivo using rat U34A Affymetrix GeneChips. In this comparison, adult male Long-Evans rats (175-200g; N=45) were housed under a standard 12h light:12h dark photoperiod (LD 12:12; lights-on at 0600 hr). more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL85

18 Samples

Download data

(Submitter supplied) To determine whether immortalized cells derived from the rat SCN (SCN 2.2) retain intrinsic rhythm-generating properties characteristic of the SCN, oscillatory properties of the SCN2.2 transcriptome were analyzed and compared to those found in the rat SCN in vivo using rat U34A Affymetrix GeneChips. SCN2.2 cells were expanded in 6-well plates. At 6-hour interval across 2 circadian cycles, cells from single 6-well plates were harvested and pooled for total RNA extraction. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL85

27 Samples

Download data: CEL, EXP, RPT

(Submitter supplied) Chick pinealocytes exhibit all the characteristics of a complete circadian system, comprising photoreceptive inputs, molecular clockworks and an easily measured rhythmic output, melatonin biosynthesis. We used microarray analysis to investigate the expression of approximately 8000 genes within cultured pinealocytes subjected to both LD and DD cycles. We report that a reduced subset of genes were rhythmically expressed in vitro compared to those previously published in vivo, and that gene expression rhythms were lower in amplitude, although the functional distribution of the rhythmic transcriptome was largely similar. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3731

196 Samples

Download data

(Submitter supplied) Summary: To identify distinct temporal patterns of mRNA expression in the kidney of rats following a bolus dose of the corticosteroid methylprednisolone. Hypothesis: That prednisone pharmacodynamics can be derived from expression profiling data. Keywords: other

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Datasets:

GDS964 GDS965

Platforms:

GPL341 GPL85

63 Samples

Download data: CEL

Expression profiling of kidneys from animals treated with methylprednisolone. Kidneys examined at various time points up to 6 hours following methylprednisolone treatment. Results provide insight into prednisone pharmacodynamics.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent, 3 time sets

Platform:

GPL85

Series:

GSE1721

10 Samples

Download data: CEL

Expression profiling of kidneys from animals treated with methylprednisolone. Kidneys examined at various time points up to 72 hours following methylprednisolone treatment. Results provide insight into prednisone pharmacodynamics.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent, 17 time sets

Platform:

GPL341

Series:

GSE1721

53 Samples

Download data: CEL

(Submitter supplied) The circadian clock is an evolutionarily conserved mechanism that drives rhythmic expression of downstream genes. The core circadian clock drives the expression of clock-controlled genes either directly or indirectly, which in turn play critical roles in carrying out many rhythmic physiological processes. Nevertheless, the molecular mechanisms by which clock output genes orchestrate rhythmic signals from the brain to peripheral tissues are largely unknown. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21306

96 Samples

Download data: TXT