GEO DataSets

(Submitter supplied) Although host genetics influences susceptibility to tuberculosis, few genes determining disease outcome have been identified. We hypothesized that macrophages from individuals with different clinical manifestations of tuberculosis infection would have distinct gene expression profiles, and that polymorphisms in these genes may also be associated with susceptibility to TB. We measured gene expression levels of >38,500 genes from ex vivo Mtb- stimulated macrophages in 12 subjects with 3 clinical phenotypes: latent, pulmonary and meningeal tuberculosis (n=4 per group). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3540

Platform:

GPL570

24 Samples

Download data: CEL

Ex vivo analysis of macrophages from individuals with a history of latent, pulmonary and meningeal tuberculosis (TB) following treatment with cell lysates of Mycobacterium tuberculosis H37Rv. Results provide insight into the molecular basis of susceptibility to TB.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 3 disease state sets

Platform:

GPL570

Series:

GSE11199

24 Samples

Download data: CEL

(Submitter supplied) Alveolar macrophages (AMs) are major targets of Mycobacterium tuberculosis (Mtb) infection, critical during the progression of active tuberculosis (TB). The complex immunopathology of TB generates diverse microenvironments in the lung, which shape immune responses by AMs. In the current study, we perform whole genome microarray transcriptional profiling on RNA isolated from AMs from TB patients (AMsTB) compared to AMs from control subjects (AMsCT) using bronchoalveolar lavage (BAL). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

7 Samples

Download data: TXT

(Submitter supplied) SP110b is an interferon (IFN)-induced nuclear protein and may function as a transcriptional co-activator/repressor. IFNγ activates monocytes/macrophages thereby mediating inflammation. However, uncontrolled activation induces monocyte/macrophage cell death, which may cause immunopathology. We have demonstrated that SP110b expression prevented IFNγ-mediated monocyte/macrophage cell death. To explore the molecular mechanisms by which SP110b suppresses IFNγ-induced cell death, we performed a genome-wide microarray analysis to identify genetic determinants associated with IFNγ-induced cell death and regulated by SP110b.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

15 Samples

Download data: CEL

(Submitter supplied) We examined the microRNA profiles of THP-1 macrophages upon the MTB infection of (1) Beijing/W and non-Beijing/W clinical strains, and (2) susceptible and multidrug-resistant (MDR-) MTB strains.

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL19763

14 Samples

Download data: TXT

(Submitter supplied) the microRNA profiles of the host macrophages were studied by microarray in a small cohort with active MTB disease, latent infection (LTBI), and from healthy controls.

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL19763

10 Samples

Download data: TXT

(Submitter supplied) The innate immune system provides the first response to pathogen infection and orchestrates the activation of the adaptive immune system. Though a large component of the innate immune response is common to all infections, pathogen-specific innate immune responses have been documented as well. The innate immune response is thought to be especially critical for fighting infection with Mycobacterium tuberculosis (MTB), the causative agent of tuberculosis (TB). more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

156 Samples

Download data: TXT

(Submitter supplied) Tuberculosis kills nearly 2 million people through out the world, every year. The outcome of M. tuberculosis infection is determined by the host and bacterial factors. A strong host immune response controls the growth of the bacilli effectively. However, in a host with suboptimal immune response, the bacilli grows and mounts disease. Activation of immune response following M. tuberculosis infection affects the expression of many host genes that are involved in the production of immune system molecules such as cytokines, chemokines, surface receptors and transcriptional regulators that manifest in the change of subsequent cellular events, including chemotaxis and proliferation of effector cells. more...

Organism:

Oryctolagus cuniculus

Type:

Expression profiling by array

Platform:

GPL13288

12 Samples

Download data: TXT

(Submitter supplied) Whole-genome expression profiling on a cohort of TB patients. Tuberculosis patients above 15 years of age were recruited from an outpatient tuberculosis clinic in central Jakarta (Indonesia). Randomly selected control subjects with the same sex and age (+/-10%) were recruited from neighboring households, with first degree relatives excluded.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6883

71 Samples

Download data: TXT

(Submitter supplied) Mycobacterium tuberculosis (Mtb) infects alveolar macrophages (AMs) causing pulmonary tuberculosis (PTB), the more frequent form of the disease. Less frequently, Mtb disseminates to many other organs and tissues resulting in different extrapulmonary forms of TB. Nevertheless, very few studies have addressed the global mRNA response of human AMs, in particular from humans with the active form of the disease. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

43 Samples

Download data: TXT

(Submitter supplied) Differences in the activity of monocytes/macrophages, important target cells of Mycobacterium tuberculosis, might influence tuberculosis progression. With the purpose of identifying candidate genes for tuberculosis susceptibility we infected with M. tuberculosis monocytes from both, healthy elders (a tuberculosis susceptibility group) and elderly tuberculosis patients, and performed a microarray experiment. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13497

15 Samples

Download data: TXT

(Submitter supplied) Identification of genetic polymorphisms associated with inter-individual variation in immune response to Mycobacterium tuberculosis infection.

Organism:

Homo sapiens

Type:

Genome variation profiling by SNP array; SNP genotyping by SNP array

Platform:

GPL8882

68 Samples

Download data: TXT

(Submitter supplied) Identification of genetic polymorphisms associated with inter-individual variation in immune response to Mycobacterium tuberculosis infection.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

259 Samples

Download data: TXT

(Submitter supplied) The W-Beijing family of Mycobacterium tuberculosis (Mtb) strains is known for its high-prevalence and -virulence, as well as for its genetic diversity, as recently reported by our laboratories and others. However, little is known about how the immune system responds to these strains. To explore this issue, here we used reverse engineering and genome-wide expression profiling of human macrophage-like THP-1 cells infected by different Mtb strains of the W-Beijing family, as well as by the reference laboratory strain H37Rv. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4258

Platform:

GPL570

40 Samples

Download data: CEL

Temporal analysis of macrophage-like THP-1 cell line infected by Mycobacterium tuberculosis (Mtb) W-Beijing strains and H37Rv. Mtb W-Beijing sublineages are highly virulent, prevalent and genetically diverse. Results provide insight into host macrophage immune response to Mtb W-Beijing strains.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 infection, 13 strain, 4 time sets

Platform:

GPL570

Series:

GSE29628

40 Samples

Download data: CEL

(Submitter supplied) Tuberculosis is a deadly infectious disease, which kills millions of people every year. The causative pathogen, Mycobacterium tuberculosis (MTB), is estimated to have infected up to a third of the world’s population; however, only approximately 10% of healthy individuals progress to active TB disease. Despite evidence for heritability, it is not currently possible to predict whether a healthy person is susceptible to TB. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20301

50 Samples

Download data: TXT

(Submitter supplied) Background: Mycobacterium tuberculosis infection is a leading cause of infectious death worldwide. Gene-expression microarray studies profiling the blood transcriptional response of tuberculosis (TB) patients have been undertaken in order to better understand the host immune response as well as to identify potential biomarkers of disease. To date most of these studies have focused on pulmonary TB patients with gene-expression profiles of extra-pulmonary TB patients yet to be compared to those of patients with pulmonary TB or sarcoidosis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

202 Samples

Download data: TXT

(Submitter supplied) Type 2 diabetes (DM2) is a disease that hinders tuberculosis (TB) control strategies worldwide. TB infection, progression, morbidity, treatment success, adverse effects, relapse, and mortality are worse in these susceptible populations. Therefore, expanding the partially known molecular mechanisms leading to TB-DM2 comorbidity may be essential to face both epidemics. Consequently, a transcriptomic study was conducted on TB-DM2 comorbidity, associated with patients with DM2 who have poor glycaemic control (PDM2), since they have a higher risk of getting TB. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10332

20 Samples

Download data: TXT

(Submitter supplied) This is a replication study on human alveolar macrophages (HAMs) from a smaller cohort from South Africa in order to support the RNA data of a relatively larger cohort from the US (Ohio & Texas). In this replication cohort, using full RNA-seq, a substantial portion of significant differentially expressed RNAs overlap with those identified with Ampliseq from the larger cohort over time, yielding similar enriched functional terms, e.g., interferon, interleukin, and IDO1 signaling.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21290

30 Samples

Download data: XLSX

(Submitter supplied) An innovative, high-throughput approach was proposed to identify key gene expression profiles and regulatory polymorphisms affecting innate immunity to TB by studying interactions between M.tb and human alveolar macrophages (AMs). We systematically analyzed interactions of a virulent M.tb strain with freshly isolated human AMs from 28 healthy adult donors, measuring host RNA expression and secreted candidate proteins associated with TB pathogenesis over 72h. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17303

124 Samples

Download data: TXT