GEO DataSets

(Submitter supplied) The proinflammatory cytokine, TNFalpha is critical in maintaining liver homeostasis since it is a major determiner of hepatocyte life and death. Considering this, gene transcription profiling was examined in control and TNFalpha treated HepG2 cells. Results indicated that TNFalpha could significantly alter the expression of a significant number of genes; most of them were functionally distributed among molecular functions like catalytic activity, binding, molecular transducer activity, transporter activity, translation and transcription regulator activities or enzyme regulator activity. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

6 Samples

Download data: CEL, CHP

(Submitter supplied) Profiling of promoter occupancy by SND1 coactivator in human hepatoma cells. Control and TNFα-treated HepG2 cells are immunoprecipitated with anti-SND1 antibody and input and immunoprecipitated material were hybridized in an Agilent human promoter microarray

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL14622

6 Samples

Download data: TXT, XLS

(Submitter supplied) Four weeks old male broilers, fed ad libitum or fasted for 16h or 48h were used to describe the evolution of global gene expression profiles in chicken liver during a 48h fasting period using a chicken 20K oligo array. Among the 20460 oligos on the microarray, 13057 were identified as aligning with a unique coding region of the 2.1 Washington University assembly of the chicken sequence genome. So the statistical analyses were performed on this 13057 gene set. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL5480

18 Samples

Download data: GPR

(Submitter supplied) Treatment of the human hepatoma cell lines HepG2, HUH7 and PHH with a mixture of fatty acids caused heaptic steatosis. The combined lipid and TNFa treatment mimics inflammation. Cells were harvested and processed for microarray analysis

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13158

33 Samples

Download data: CEL, CHP

(Submitter supplied) In this study, RNA-Seq technology was adopted to investigate the differences in expression profiles of the hepatic lipid metabolism-related genes and the associated pathways between juvenile and laying hens. RNA-Seq analysis was carried out to estimate total RNA harvested from the liver of juvenile hens (n = 3) and laying hens (n = 3). Compared with juvenile hens, 2574 differentially expressed (DE) genes (1487 down and 1087 up) with P ≤ 0.05 were obtained, and 955 of these genes were significantly DE (SDE) at a false discovery rate (FDR) of 0.05 and fold-change ≥ 2 in laying hens. more...

Organism:

Gallus gallus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19005

6 Samples

Download data: TXT

(Submitter supplied) Social stress is well known to be involved in the occurrence and exacerbation of mental illness, and also various life-style related diseases such as hyperinsulinemia, hyperglycemia, cardiovascular diseases and cancer. However, there is little information on tissue-specific gene expression in response to social stress, which reflects our daily life. Liver is one of the most important organs, owing to its biological functions such as energy metabolic homeostasis, metabolization and detoxification of endo- and exogenous substances. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL, CHP

(Submitter supplied) Cationic liposomes (CLs) are non-viral vectors that play a major role in DNA transfer and gene therapy. The cellular toxicity of CLs has been validated in HepG2 cells. To address the basis of the CLs toxicity, we performed RNA-seq analysis on untreated HepG2 cells and HepG2 cells following exposure to IC50 concentration (120μM) CLs for 24h.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17303

6 Samples

Download data: TXT

(Submitter supplied) The objective was to evaluate the status of the global hepatic transcriptome in the presence of inhibited calreticulin levels. Results identify a total of 324 genes were altered of which 245 genes were downregulated and 79 genes were upregulated. This suggests that such altered genes might be associated with altered hepatic physiology that are observed during calreticulin deficiency.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

6 Samples

Download data: TXT

(Submitter supplied) The effects of TNFɑ [40 ng/ml] ± Apigenin [40 μM] were evaluated in MDA-MB-231 cells using GeneChip™ Human Gene 2.1 ST Arrays by Affymetrix Inc assessing for mRNAs and long intergenic non-coding RNA transcripts.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17692

12 Samples

Download data: CEL, CHP

(Submitter supplied) 1507 known genes have been identified differentially regulated during HisOH treatment by more than 2 fold. This includes 250 down-regulated genes and 1257 up-regulated genes.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

8 Samples

Download data: CEL

(Submitter supplied) Expression profiling the response to inhibition of DNA methylation and histone deacetylation. Comparison of expression in HepG2 cells treated with 5-aza-dC, Trichostatin A, both, or none (control) to change methylation and acetylation status. Background:DNA methylation and histone deacetylation are epigenetic mechanisms that play major roles in eukaryotic gene regulation. We hypothesize that many genes in the human hepatoma cell line HepG2 are regulated by DNA methylation and histone deacetylation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2213

Platform:

GPL570

16 Samples

Download data

Analysis of HepG2 hepatoma cells following treatment with 5-aza-2'-deoxycytidine (5-aza-dC) to inhibit DNA methylation, or trichostatin A (TSA) to inhibit histone deacetylation, or both. Results identify epigentically regulated genes in hepatoma cells.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 agent sets

Platform:

GPL570

Series:

GSE5230

16 Samples

Download data

(Submitter supplied) Transcriptional profiling of mouse livers comparing control mice with miR-155 transgenic mice that overexpression of miR-155 in the liver

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL13509

6 Samples

Download data: LSR, XLS

(Submitter supplied) The sre1 pathway senses molecular oxygen in the fission yeast, Schizosaccharomyces pombe. When oxygen becomes limiting, Sre1 is trafficked from the ER to the Golgi where it is cleaved through the actions of the 5-member Dsc E3 ligase complex. The released N-terminus then upregulates genes required for adaptation to hypoxia. Although the sre1 pathway is responsible for regulating a majority of the genes required for hypoxic growth, it is evident that other regulatory genes are involved in this response. To identify novel regulators of adaptation to hypoxia, we screened the S. more...

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by array

Platform:

GPL19098

2 Samples

Download data: TXT

(Submitter supplied) To understand the molecular basis underlying the response to increasing CD36 overexpression in HepG2.2.15 cell, we performed genome-wide sequencing of the mRNAs from HepG2.2.15-CD36OE cell and HepG2.2.15-vector cell using the RNA-seq technology to detect its differential transcriptomic profile.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17303

6 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:

GPL11154

12 Samples

Download data: BED

(Submitter supplied) The interplay between mitogenic and proinflammatory signaling pathways play key roles in determining the phenotypes and clinical outcomes of breast cancers. We have used global nuclear run-on coupled with deep sequencing to characterize the immediate transcriptional responses of MCF-7 breast cancer cells treated with estradiol, TNFα, or both. In addition, we have integrated these data with chromatin immunoprecipitation coupled with deep sequencing for estrogen receptor alpha (ERα), the pioneer factor FoxA1 and the p65 subunit of the NF-κB transcription factor. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

12 Samples

Download data: BED

(Submitter supplied) The interplay between mitogenic and proinflammatory signaling pathways play key roles in determining the phenotypes and clinical outcomes of breast cancers. We have used global nuclear run-on coupled with deep sequencing to characterize the immediate transcriptional responses of MCF-7 breast cancer cells treated with estradiol, TNF?, or both. In addition, we have integrated these data with chromatin immunoprecipitation coupled with deep sequencing for estrogen receptor alpha (ER?), the pioneer factor FoxA1 and the p65 subunit of the NF-?B transcription factor. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL16791

40 Samples

Download data: BED

(Submitter supplied) Rheumatoid arthritis synovial fibroblasts (RASF) were transected with an adenovirus (Ad) expressing a dominant negative (DN) form of Ik-B stimulated 18 hours later with TNF-a. Controls were RASF transfected with Adtet then treated with TNF-a 18 hours later. RNA was extracted 3 hours after TNF-a application. The same cell line was split into 6 lots grown up and then treated with the virus independent. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS486

Platform:

GPL8300

6 Samples

Download data

Analysis of rheumatoid arthritis synovial fibroblasts (RASF) expressing dominant negative form of inhibitor of nuclear factor kappa B (Ik-B). Ik-B blocks TNFalpha. Cells treated with TNFalpha at 18 hours. TNFalpha known to increase progression of RA.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 cell line sets

Platform:

GPL8300

Series:

GSE766

6 Samples

Download data