GEO DataSets

(Submitter supplied) Ischemia exists in many diseased tissues including arthritic joints, atherosclerotic plaques and malignant tumors. Macrophages accumulate in these sites and upregulate genes in response to the hypoxia present. We used microarrays to detail the hypoxia upregulated gene in human primary macrophages.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

2 Samples

Download data: CEL, CHP

(Submitter supplied) Primary human macrophages with a HIF-1alpha or HIF-2alpha knockdown were pretreated with IL-10 for 16h and afterwards for 4h additionaly under hypoxi (1% O2), RNA was isolated usind the Qiagen RNAeasy Kit and cDNA synthesis wos done using Ambion WT Expression Kit. Expression was compared to si control under control conditions.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

36 Samples

Download data: CEL

(Submitter supplied) Neutrophils are key effector cells of the innate immune response and are required to migrate and function within highly adverse micro-environmental conditions. These inflammatory sites are characterised by low levels of oxygen. We examined the relative transcript abundance detected in an apoptosis targeted gene array following invitro neutrophil culture (3/6 hrs) in normoxic, hypoxic and anoxic conditions. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL931

18 Samples

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(Submitter supplied) Gene expression profiles of 39 human sarcoma samples (GSM 52571-GSM52609) and 15 control samples (GSM52556-GSM52570) were assessed using Affymetrix HG U133A oligonucleotide arrays. Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1209

Platform:

GPL96

54 Samples

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Expression profiling of soft tissue sarcoma samples. Hypoxic regions often develop in tumors as they increase in size. Results provide insight into the expression of hypoxia-related genes in sarcomas.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state, 23 tissue sets

Platform:

GPL96

Series:

GSE2719

54 Samples

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(Submitter supplied) Cell lines. HEK293 human embryonic kidney cells were obtained from ATCC (American Type Culture Collection, Manassas, VA) and were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (Heat inactivated, Hyclone, Logan, Utah) and 1X antibiotics/glutamine (100 units/ml penicillin, 100ug/ml streptomycin, 292ug/ml L-Glutamine sulfate, all from Invitrogen Corp, Carlsbad, CA.), under either hypoxic (1% O2) or normoxic (21% O2) conditions at 37C in a tissue culture incubator. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2018

Platform:

GPL1528

10 Samples

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Analysis of HEK293T embryonic kidney cells subjected to hypoxia or overexpressing hypoxia-inducible factor (HIF)-1, degradation resistant HIF-1, or HIF-2. Results provide insight into the respective roles of HIF-1 and HIF-2 in the cellular response to hypoxia.

Organism:

Homo sapiens

Type:

Expression profiling by array, log2 ratio, 4 protocol sets

Platform:

GPL1528

Series:

GSE2020

10 Samples

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(Submitter supplied) The response of cells to hypoxia is characterised by co-ordinated regulation of many genes. Studies of the regulation of the expression of many of these genes by oxygen has implicated a role for the heterodimeric transcription factor hypoxia inducible factor (HIF). The mechanism of oxygen sensing which controls this heterodimeric factor is via oxygen dependent prolyl and asparaginyl hydroxylation by specific 2-oxoglutarate dependent dioxygenases (PHD1, PHD2, PHD3 and FIH-1). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS2758 GDS2759 GDS2760 GDS2761

Platforms:

GPL2507 GPL96 GPL570

39 Samples

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Analysis of hypoxic MCF-7 breast cancer cells depleted for hypoxia-inducible factor (HIF)-1alpha, HIF-2alpha, or both HIF alpha subunits. Results provide insight into the extent of the role of HIF-1alpha and HIF-2alpha in the regulation of gene expression during hypoxia.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 protocol sets

Platform:

GPL2507

Series:

GSE3188

12 Samples

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Analysis of hypoxic MCF-7 breast cancer cells depleted for hypoxia-inducible factor (HIF)-1alpha, HIF-2alpha, or both HIF alpha subunits. Results provide insight into the extent of the role of HIF-1alpha and HIF-2alpha in the regulation of gene expression during hypoxia.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 protocol sets

Platform:

GPL570

Series:

GSE3188

12 Samples

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Analysis of MCF-7 breast cancer cells exposed to hypoxia or treated with the non-specific 2-oxoglutarate (2-OG) dependent dioxygenase inhibitor dimethyloxalylglycine. Results provide insight into the role of 2-OG dioxygenases in the regulation of gene expression during hypoxia.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 3 protocol sets

Platform:

GPL2507

Series:

GSE3188

6 Samples

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Analysis of MCF-7 breast cancer cells exposed to hypoxia or treated with the non-specific 2-oxoglutarate (2-OG) dependent dioxygenase inhibitor dimethyloxalylglycine. Results provide insight into the role of 2-OG dioxygenases in the regulation of gene expression during hypoxia.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 protocol sets

Platform:

GPL96

Series:

GSE3188

9 Samples

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