GEO DataSets

(Submitter supplied) Cellular dedifferentiation signifies the withdrawal of cells from a specific differentiated state into a ‘stem cell’-like undifferentiated state. However, the mechanism of dedifferentiation remains obscure. We showed that mature adipocytes (MA) and follicular granulosa cells (GC), which have distinct functions in vivo, can dedifferentiate during culture in vitro and acquire multipotency. We investigated the dedifferentiation mechanism of MA and GC using global gene expression analyses.

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL3533

12 Samples

Download data: CEL

(Submitter supplied) DNA microarray analysis was performed with mouse multipotent adult germline stem cells (maGSCs) and embryonic stem cells (ESCs) from different genetic backgrounds cultured under standard ESC culture conditions and under differentiation-promoting conditions by the withdrawal of Leukemia Inhibitory Factor (LIF) and treatment with Retinoic Acid (RA). The analyzed undifferentiated cell lines are very similar based on their global gene expression pattern and show 97-99% identity dependent on the analyzed background. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4170

Platform:

GPL6246

18 Samples

Download data: CEL, CHP

Analysis of multipotent adult germline stem cells (maGSCs) and embryonic stem cells (ESCs) from different genetic backgrounds and under differentiation-promoting conditions. Results enable expression pattern comparisons between maGSCs and ESCs.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 cell type, 2 growth protocol, 2 strain sets

Platform:

GPL6246

Series:

GSE22605

18 Samples

Download data: CEL, CHP

(Submitter supplied) Directing differentiation of human embryonic stem cells (hESC) into specific cell types using an easy and reproducible protocol is a perquisite for the clinical use of hESC in regenerative medicine protocols. Here, we report the generation of mesodermal cells with differentiation potential to myocytes, osteoblasts, chondrocytes and adipocytes. We demonstrate that during hESC differentiation as embryoid bodies (EB), inhibition of TGF-b/Activin/Nodal signaling using SB-431542 (SB) markedly up-regulated paraxial mesodermal markers (TBX6, TBX5), early myogenic transcriptional factors (Myf5, Pax7) as well as myocyte committed markers (NCAM, CD34, Desmin, MHC (fast), alpha-smooth muscle actin, Nkx2.5, cTNT). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

2 Samples

Download data: CEL, CHP

(Submitter supplied) Adipose tissue is a plentiful and easily accessible source of mesenchymal stem cells that have been shown to be multi potent and possibly have immuno suppressive capacity(Zuk, Zhu et al. 2002; McIntosh, Zvonic et al. 2006; Prichard, Reichert et al. 2008). Several studies have identified hypoxia and the molecular effector of reduced oxygen tension, HIF1α to be essential in the differentiation processes, stem cell proliferation and survival as well as in maintenance of stem cell characteristics (Lennon, Edmison et al. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL7350

29 Samples

Download data: TXT

(Submitter supplied) The possibility of generating neural cells from human bone marrow-derived mesenchymal stem cells by simple in vitro treatments is alluring both conceptually and practically. However, whether phenotypic modulations observed after chemical manipulation of such stem cells truly represent a genuine trans-lineage differentiation remains to be established. We have re-evaluated the effects of a frequently reported biochemical approach, based on treatment with butylated hydroxyanisole and dimethylsulphoxide, to bring about such phenotypic conversion by monitoring the morphological changes induced by the treatment in real time, by analysing the expression of phenotype-specific protein markers and by assessing the modulation of the cells’ transcriptome. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1347

Platform:

GPL2136

11 Samples

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(Submitter supplied) This set consists of 21,329 70mer oligonucleotides (Operon version 2.0) designed on Human Unigene clusters, mainly in the 3 end terminal region. Each oligonucleotide is spotted by our robotic station Biorobotics Microgrid II, in two replicates on a MICROMAX glass slide SuperChip I (Cat No MPS696) provided by PerkinElmer Life Sciences Inc. The deposition is qualitatively assessed by a scan of the spotted slide at 10 micron resolution, using a GSI Lumonics scanner and a protocol that allows the detection of spotted features through the salts derived from the dried solution in which oligonucleotides are dissolved. more...

Organism:

Homo sapiens

1 DataSet

4 Series

39 Samples

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Comparison of mesenchymal stem cells (MSC) treated with dimethylsulphoxide and butylated hydroxyanisole (DMSO/BHA) for 6 or 48 hours to fetal brain (positive control) or adult liver (negative control). Results raise doubts about the DMSO/BHA protocol for generating neural cells from MSCs.

Organism:

Homo sapiens

Type:

Expression profiling by array, log2 ratio, 2 agent, 3 cell type, 3 time sets

Platform:

GPL2136

Series:

GSE2776

11 Samples

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(Submitter supplied) Comparisons of expression profils of human undiferentiated ES cells and Mesenchymal ES cells Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1288

Platform:

GPL96

6 Samples

Download data: CEL

Comparison of mesenchymal stem cells (MSC) and undifferentiated embryonic stem cells (ESC). ESC plated on a monolayer of OP9 stromal cells in the presence of 20% heat inactivated fetal bovine serum in alpha MEM medium to induce differentiation into MSC.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 cell type sets

Platform:

GPL96

Series:

GSE2248

6 Samples

Download data: CEL

(Submitter supplied) Mesenchymal stromal cells (MSCs) are promising candidates for innovative cell therapeutic applications. For clinical scale manufacturing regulatory agencies recommend to replace fetal bovine serum (FBS) commonly used in MSC expansion media as soon as equivalent alternative supplements are available. We already demonstrated that pooled blood group AB human serum (HS) and thrombin-activated platelet releasate plasma (tPRP) support the expansion of multipotent adipose tissue-derived MSCs (ASCs). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1708

21 Samples

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(Submitter supplied) Purpose: The goals of this study are to compare the transcription changes of dermal adipocytes during hair cycling. Methods: Transcriptome of purified dermal adipocytes were generated by deep sequencing, using Illumina Novaseq 6000 sequencing system.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

15 Samples

Download data: TXT

(Submitter supplied) Purpose: The goals of this study are to compare the de-differentiated dermal adipocytes with normal skin fibroblasts. Methods: Library prepared followed by 10X Genomics standard protocol. Transcriptome was generated by high throughput sequencing.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

1 Sample

Download data: MTX, TSV

(Submitter supplied) Purpose: The goals of this study are to compare the transcription difference between purified dermal adipocytes and inguinal adipocytes Methods: Transcriptome of purified dermal adipocytes and inguinal adipocytes were generated by deep sequencing, using Illumina Hiseq 2500 v3 sequencing system.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

4 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Dictyostelium discoideum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26360

154 Samples

Download data: CSV

(Submitter supplied) Transcriptomic analysis of dedifferentiation in Dictyostelium discoideum: comparing wild type with bzpS-, mybD-, nfyA-, DDB_G0281091-, DDB_G0269374- and DDB_G0272386- mutant cells dedifferentiating in growth medium

Organism:

Dictyostelium discoideum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26360

42 Samples

Download data: TXT, XLS

(Submitter supplied) Transcriptomic analysis of dedifferentiation in Dictyostelium discoideum by single cell RNAseq

Organism:

Dictyostelium discoideum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26360

4 Samples

Download data: CSV

(Submitter supplied) Transcriptomic analysis of dedifferentiation in Dictyostelium discoideum: comparing wild type and forG- (formin-) mutant cells dedifferentiating in growth medium

Organism:

Dictyostelium discoideum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26360

24 Samples

Download data: TXT, XLS

(Submitter supplied) Transcriptomic analysis of dedifferentiation in Dictyostelium discoideum, with comparison to forward development

Organism:

Dictyostelium discoideum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26360

84 Samples

Download data: TXT, XLS