GEO DataSets

(Submitter supplied) Mating compatibility in C. cinerea is controlled by two loci, A and B. Following fusion of undifferentiated hyphal cells, a complex program is initiated which results in the maintenance of one nucleus from each parent in every cell (the dikaryon). We identified downstream targets of the A locus (A on), the B locus (B on), and both loci (Aon Bon) using strains in which the two pathways are activated separately, and a dikaryotic strain. more...

Organism:

Coprinopsis cinerea

Type:

Expression profiling by array

Platform:

GPL7697

12 Samples

Download data: GPR

(Submitter supplied) Coprinopsis cinerea vegetative dikaryotic mycelia compared to vegetative monokaryotic mycelia

Organism:

Coprinopsis cinerea

Type:

Expression profiling by array

Platform:

GPL7697

3 Samples

Download data: GPR, TIFF

(Submitter supplied) Coprinopsis cinerea exhibits synchronised meiosis in the gill tissue of the fungus, which is 75% meiotic. The mushroom develops from a dikaryon, which contains two separate nuclei. These nucleifuse in the basidia (karyogamy). After karyogamy, the nuclei enter an extended meiotic prophase, in which pahcytene occurs at 6 hours post karyogamy (K+6). The tetrads produced by the second meiotic division are present 12 hours after karyogamy (K+12). more...

Organism:

Coprinopsis cinerea

Type:

Expression profiling by array

Platform:

GPL7697

24 Samples

Download data: GPR

(Submitter supplied) Large scale assessment of the transcriptomes of the vegetative mycelium and primordium will facilitate the generation of a more comprehensive picture of the fruiting process in basidiomycetes. We coupled 5'-Serial Analysis of Gene Expression (5'-SAGE) to high-throughput pyrosequencing from 454 Life Sciences to analyze the transcriptomes and identify up-regulated genes (URGs) among vegetative mycelium (Myc) and stage 1 primordium (S1-Pri) of Coprinopsis cinerea during fruiting body development. more...

Organism:

Coprinopsis cinerea

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16031

2 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Coprinopsis cinerea

Type:

Expression profiling by array

Platform:

GPL7697

44 Samples

Download data: GPR

(Submitter supplied) Meiosis was compared in msh5-22 and wild type strains of C. cinerea at 6 time points spanning the meiotic timecourse. Abstract: The basidiomycete Coprinopsis cinerea is well-suited to studies of meiosis because meiosis progresses synchronously in ten million cells within each mushroom cap. Approximately 20% of C. cinerea genes exhibit changing expression during meiosis, but meiosis and mushroom development happen concurrently so differentially expressed genes might not be directly involved in meiotic processes. more...

Organism:

Coprinopsis cinerea

Type:

Expression profiling by array

Platform:

GPL7697

24 Samples

Download data: GPR

(Submitter supplied) Meiosis was compared in rad50-1 and wild type strains of C. cinerea at 6 time points spanning the meiotic timecourse. Abstract: The basidiomycete Coprinopsis cinerea is well-suited to studies of meiosis because meiosis progresses synchronously in ten million cells within each mushroom cap. Approximately 20% of C. cinerea genes exhibit changing expression during meiosis, but meiosis and mushroom development happen concurrently so differentially expressed genes might not be directly involved in meiotic processes. more...

Organism:

Coprinopsis cinerea

Type:

Expression profiling by array

Platform:

GPL7697

20 Samples

Download data: GPR

(Submitter supplied) The Transcriptome of different tissues and developmental stages of Laccaria bicolor S238N was analyzed. The array probes were designed from gene models taken from the Joint Genome institute Laccaria bicolor genome sequence version1. One aim of this study was to verify the expression of the automatically annotated gene models in various tissues and to use this transcriptional information to confirm, to correct or to reject gene models. more...

Organism:

Populus tremula x Populus alba; Laccaria bicolor S238N-H82; Populus trichocarpa; Laccaria bicolor; Pseudotsuga menziesii

Type:

Expression profiling by array

Platform:

GPL6192

11 Samples

Download data: TXT

(Submitter supplied) To identify genes express in earliest stage of fruiting body initiation in Coprinopsis cinerea, strain #326 (AmutBmut haploid fruiting strain), genes expressed at 0, 0.5, 1, 3h after light exposure were compared by Super-SAGE Approximately, 1 million tags were sequenced from each mycelial sample to obtain 65,535 unique tags. First, we made matches between the tags and predicted genes of the WT strain, and 24.4% tags matched to predicted genes. more...

Organism:

Coprinopsis cinerea

Type:

Expression profiling by SAGE

Platform:

GPL23540

4 Samples

Download data

(Submitter supplied) Transcript profiles of Laccaria bicolor S238N mycelium on various media were analyzed. The array probes were designed from gene models taken from the Joint Genome Institute (JGI, department of energy) Laccaria bicolor genome sequence version 1. One goal was to evaluate the effect of nutrient deprivation on the transcriptome of Laccaria bicolor.

Organism:

Laccaria bicolor S238N-H82; Laccaria bicolor

Type:

Expression profiling by array

Platform:

GPL14641

15 Samples

Download data: PAIR

(Submitter supplied) The morphogenesis of sexual fruiting bodies of fungi is a complex process determined by a genetically encoded programme. Fruiting bodies reached highest complexity levels in the Agaricomycetes, yet, the underlying genetics is currently poorly known. In this work, we functionally characterised an unannotated gene we term snb1, whose expression level increases rapidly during fruiting body initiation. According to phylogenetic analyses, orthologues of snb1 are present in almost all Agaricomycetes and may represent a novel conserved gene family that plays a substantial role in fruiting body development. We disrupted snb1 using CRISPR/Cas9 in the agaricomycete model organism Coprinopsis cinerea. Snb1 mutants formed unique, snowball-shaped, rudimentary fruiting bodies that could not differentiate caps, stipes and lamellae. We took advantage of this phenotype to study fruiting body differentiation using RNA-Seq analyses. This revealed a multitude of differentially regulated genes and gene families that may be related to tissue differentiation and the formation of structures. Taken together, the novel gene family of snb1 and the differentially expressed genes in the snb1 mutants provide valuable insights into the complex mechanisms underlying tissue differentiation in the Agaricomycetes.

Organism:

Coprinopsis cinerea

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33837

12 Samples

Download data: CSV, FASTA, GTF