GEO DataSets

(Submitter supplied) Staphylococcus aureus is a leading cause of bloodstream infections worldwide. In the United States, many of these infections are caused by a strain known as USA300. Although progress has been made, our understanding of the S. aureus molecules that promote bacteremia and survival in human blood is incomplete. To that end, we analyzed the USA300 transcriptome during culture in human blood, human serum, and trypticase soy broth (TSB), a standard laboratory culture media. more...

Organism:

Staphylococcus aureus; Staphylococcus aureus subsp. aureus USA300

Type:

Expression profiling by array

Platform:

GPL8069

80 Samples

Download data: CEL, CHP

(Submitter supplied) Staphylococcus aureus strain MW2 was exposed to the following neutrophil microbicides, hydrogen peroxide (H2O2), hypochlorous acid (HOCl) and azurophilic granule proteins. At the indicated time points, bacterial cultures were centrifuged and bacteria were lysed with RLT buffer (Qiagen) using a FastPrep system. Purification of MW2 RNA and subsequent preparation of labeled cDNA target was performed as described in Methods. more...

Organism:

Coxiella burnetii; Staphylococcus epidermidis ATCC 12228; Chlamydia trachomatis D/UW-3/CX; Staphylococcus aureus; Chlamydia pneumoniae AR39; Borreliella burgdorferi B31; Coxiella burnetii RSA 493; Chlamydia caviae GPIC; Staphylococcus haemolyticus JCSC1435; Rickettsia rickettsii; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Staphylococcus aureus subsp. aureus MW2; Granulibacter bethesdensis

Type:

Expression profiling by array

Platform:

GPL4692

48 Samples

Download data: CEL

(Submitter supplied) Panton-valentine leukocidin (PVL) has been linked to worldwide emergence of community-associated methicillin resistant Staphylococcus aureus (CA-MRSA) -- its role in virulence in unclear. Here we show that PVL had no effect on global gene expression of prominent CA-MRSA strains nor did it affect bacterial clearance from lungs, spleen and kidneys in a highly discriminatory rabbit bacteremia model. These findings negate a large body of epidemiological research that implicated PVL in CA-MRSA virulence. more...

Organism:

Borreliella burgdorferi; Yersinia pestis; Chlamydia pneumoniae AR39; Coxiella burnetii RSA 493; Granulibacter bethesdensis; Chlamydia trachomatis; Borreliella burgdorferi B31; Chlamydia trachomatis D/UW-3/CX; Staphylococcus haemolyticus JCSC1435; Streptococcus pyogenes; Staphylococcus epidermidis ATCC 12228; Staphylococcus aureus subsp. aureus MW2; Coxiella burnetii; Rickettsia rickettsii; Staphylococcus aureus; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Chlamydia caviae GPIC

Type:

Expression profiling by array

Platforms:

GPL2129 GPL4692

30 Samples

Download data: CEL, CHP

(Submitter supplied) The Staphylococcus aureus Panton Valentine leukocidin (PVL) is a pore-forming toxin secreted by strains epidemiologically associated with the current outbreak of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) and with the often lethal necrotizing pneumonia. To investigate the role of PVL in pulmonary disease, we tested the pathogenicity of clinical isolates, isogenic PVL-negative and PVL-positive S. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL4653

12 Samples

Download data: TXT

(Submitter supplied) Methicillin resistant Staphylococcus aureus (MRSA) is an opportunistic pathogen chief amongst bloodstream infecting pathogens. MRSA produces an array of human specific virulence factors that may contribute to immune suppression. Here, we defined the response of primary human phagocytes to infection with MRSA using RNA-Seq. We found that the overall transcriptional response to MRSA was weak both in the number of genes and the magnitude of response. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL20301 GPL24676

114 Samples

Download data: TXT

(Submitter supplied) Methicillin-resistant Staphylococcus aureus (MRSA) is problematic both in hospitals and the community. Currently, we have limited understanding of mechanisms of innate immune evasion used by S. aureus. To that end, we created an isogenic deletion mutant in strain MW2 (USA400) of the saeR/S two-component gene regulatory system and studied its role in mouse models of pathogenesis and during human neutrophil interaction. more...

Organism:

Staphylococcus aureus; Chlamydia muridarum; Staphylococcus epidermidis RP62A; Chlamydia caviae GPIC; Staphylococcus haemolyticus JCSC1435; Coxiella burnetii; Rickettsia rickettsii; Borreliella burgdorferi B31; Coxiella burnetii RSA 493; Chlamydia trachomatis D/UW-3/CX; Chlamydia pneumoniae AR39; Staphylococcus epidermidis ATCC 12228; Staphylococcus aureus subsp. aureus MW2; Granulibacter bethesdensis

Type:

Expression profiling by array

Platform:

GPL4692

12 Samples

Download data: CEL, CHP

(Submitter supplied) Neutrophil lysis after phagocytosis is a process potentially important in the pathogenesis of community-associated methicillin-resistant S. aureus (CA-MRSA) infection. The mechanism for this process is not currently known. Therefore, to better understand CA-MRSA virulence we used human oligonucleotide microarrays to investigate the mechanism underlying enhanced PMN lysis that occurs after phagocytosis of CA-MRSA. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

113 Samples

Download data: CEL, CHP

(Submitter supplied) It remains unclear how the bacterial populations which colonise many healthy humans occasionally give rise to severe disease. Staphylococcus aureus represents one such population. Here we describe mutations in invasive S. aureus which arose during human colonisation and inactivated rsp, a transcription factor. RNA-Seq was used to generate mRNA profiles of genes controlled by rsp.

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17452

12 Samples

Download data: TXT

(Submitter supplied) Transcriptional start sites determination of USA300 (JE2) by using dRNA-Seq

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19006

4 Samples

Download data: WIG

(Submitter supplied) Staphylococcus aureus CBS2016-05 and PS/BAC/317/16/W were spiked (4E+06 CFU/ml) into platelet concentrates (PCs) and Trypticase soy broth (TSB) and allowed to grow until early stationary phase (6 days) at 22-24°C. Subsequently, the cells were pelleted at 4°C and subjected to RNA extraction using the miRNeasy Mini Kit, following the manufacturer's instructions. To eliminate genomic DNA, the RNA samples from three independent biological replicates were treated with TURBO™ DNase AmbionTM (Thermo Fisher Scientific).

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24034

12 Samples

Download data: XLSX

(Submitter supplied) Microarray expression profiling of S. aureus lavaged from murine lungs after residence times between 30 minutes and 6 hours compared to post-exponential phase or early log phase growth in Luria-Betani broth.

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL4047

21 Samples

Download data: CEL

(Submitter supplied) Genes that showed altered expression in Stk1 and Stp1 deficient Staphylococcus aureus Newman were identified. strain comparison, global regulation

Organism:

Staphylococcus aureus subsp. aureus COL; Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL10347

3 Samples

Download data: TXT

(Submitter supplied) We have studied the transcriptome of S. aureus SH1000 strain, isogenic Teg49 mutant strain, and isogenic plasmid complemented strain

Organism:

Staphylococcus aureus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24034

6 Samples

Download data: CSV

(Submitter supplied) Our previous studies identified a population of stem cell-like proliferating myeloid cells within inflamed tissues that could serve as a reservoir for tissue macrophages to adopt different activation states depending on the microenvironment. By lineage tracing cells derived from CX3CR1+ precursors in mice during infection and profiling by scRNA-seq, here we identify a cluster of BIRC5+ myeloid cells that expanded in the liver during either chronic infection with the parasite Schistosoma mansoni or the bacterial pathogen Staphylococcus aureus. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

16 Samples

Download data: MTX, TSV