GEO DataSets

(Submitter supplied) AdpA is a global transcriptional activator triggering morphological differentiation and secondary metabolism in Streptomyces griseus. AdpA influences expression of >1,000 genes, but the overall picture of AdpA regulon has been obscure. Here, we took snapshots of distribution of AdpA across the chromosome in living S. griseus cells by ChIP/ChAP-seq analysis. In both liquid and solid cultures, AdpA bound to similar >1,200 sites, which were located on not only putative regulatory regions (65 %) but also regions (35 %) that appeared not to affect transcription. more...

Organism:

Streptomyces griseus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL14940

3 Samples

Download data: SAM

(Submitter supplied) AdpA is a global transcriptional activator triggering morphological differentiation and secondary metabolism in Streptomyces griseus. AdpA influences expression of >1,000 genes, but the overall picture of AdpA regulon has been obscure. Here, we took snapshots of distribution of AdpA across the chromosome in living S. griseus cells by ChIP/ChAP-seq analysis. In both liquid and solid cultures, AdpA bound to similar >1,200 sites, which were located on not only putative regulatory regions (65 %) but also regions (35 %) that appeared not to affect transcription. more...

Organism:

Streptomyces griseus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL14940

2 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Streptomyces griseus

Type:

Expression profiling by array; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL5473 GPL14940

9 Samples

Download data: SAM, TXT

(Submitter supplied) To identify genes regulated by AdpA in liquid culture, transcriptome profiles between delta adpA strains with his-adpA and control delta adpA strains were analyzed.

Organism:

Streptomyces griseus

Type:

Expression profiling by array

Platform:

GPL5473

1 Sample

Download data: TXT

(Submitter supplied) To identify genes regulated by AdpA in solid culture, transcriptome profiles between delta adpA strains with his-adpA and control delta adpA strains were analyzed.

Organism:

Streptomyces griseus

Type:

Expression profiling by array

Platform:

GPL5473

3 Samples

Download data: TXT

(Submitter supplied) Comparative hybridization of wild type versus AdpA deficient mutant grown on SMM medium

Organism:

Streptomyces griseus; Streptomyces griseus subsp. griseus NBRC 13350

Type:

Expression profiling by array

Platform:

GPL5473

3 Samples

Download data: TXT

(Submitter supplied) BldD is a transcriptional regulator essential for morphological development and antibiotic production in Streptomyces coelicolor. Here we identify the BldD regulon by means of chromatin immunoprecipitation. The BldD regulon encompasses ~167 transcriptional units, of which more than 20 are known to play important roles in development and/or secondary metabolism. Strikingly, 42 BldD target genes (~25% of the regulon) encode regulatory proteins, stressing the central, pleiotropic role of BldD. more...

Organism:

Streptomyces coelicolor

Type:

Genome binding/occupancy profiling by array

Platform:

GPL10757

4 Samples

Download data: TXT

(Submitter supplied) Comparative hybridization of afsA deficient mutant versus afsA deficient mutant after A-factor addition

Organism:

Streptomyces griseus; Streptomyces griseus subsp. griseus NBRC 13350

Type:

Expression profiling by array

Platform:

GPL5473

24 Samples

Download data

(Submitter supplied) Comparative hybridization of wild type versus AdpA deficient mutant Keywords: Cell type comparison

Organism:

Streptomyces griseus subsp. griseus NBRC 13350; Streptomyces griseus

Type:

Expression profiling by array

Platform:

GPL5473

4 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Synechocystis sp. PCC 6803

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platforms:

GPL23245 GPL23246

8 Samples

Download data: BW, TXT

(Submitter supplied) Cyanobacteria are oxygenic photoautotrophs responsible for a substantial proportion of nitrogen fixation and primary production in the hydrosphere. Non-nitrogen fixing cyanobacteria, such as Synechocystis sp. PCC 6803, depend of the availability of nitrogenized species to survive. Therefore, an intricate regulatory network around the transcriptional factor NtcA maintains the homeostasis of nitrogen in these organisms. more...

Organism:

Synechocystis sp. PCC 6803

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23246

4 Samples

Download data: TXT

(Submitter supplied) Cyanobacteria are oxygenic photoautotrophs responsible for a substantial proportion of nitrogen fixation and primary production in the hydrosphere. Non-nitrogen fixing cyanobacteria, such as Synechocystis sp. PCC 6803, depend of the availability of nitrogenized species to survive. Therefore, an intricate regulatory network around the transcriptional factor NtcA maintains the homeostasis of nitrogen in these organisms. more...

Organism:

Synechocystis sp. PCC 6803

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL23245

4 Samples

Download data: BW

(Submitter supplied) We have used chromatin immunoprecipitation followed by microarray analysis (chIP-chip) to identify DNA regions bound by the ChvI protein in Sinorhizobium meliloti cells. We then used quantitative PCR with chvI mutant strains to test the ChvI-dependent expression of genes downstream of the ChvI-bound DNA regions.

Organism:

Sinorhizobium meliloti; Medicago truncatula

Type:

Genome binding/occupancy profiling by array

Platform:

GPL9757

9 Samples

Download data: CEL

(Submitter supplied) To gain knowledge on L. monocytogenes differential CodY activity, we set on analyzing CodY’s regulon in L. monocytogenes in both rich and minimal growth conditions using genome wide sequencing techniques. Remarkably, we identified for the first time a global regulatory role for CodY when BCAAs are limited, that are similar to those within the mammalian niche. Furthermore, our data establish CodY as a central regulator that integrates metabolism, motility, stress responses and virulence in L. more...

Organism:

Listeria monocytogenes

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL21330

8 Samples

Download data: XLSX

(Submitter supplied) To gain knowledge on L. monocytogenes differential CodY activity, we set on analyzing CodY’s regulon in L. monocytogenes in both rich and minimal growth conditions using genome-wide sequencing techniques. Remarkably, we identified for the first time a global regulatory role for CodY when BCAAs are limited, that are similar to those within the mammalian niche. Furthermore, our data establish CodY as a central regulator that integrates metabolism, motility, stress responses and virulence in L. more...

Organism:

Listeria monocytogenes 10403S

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20912

11 Samples

Download data: XLSX

(Submitter supplied) In this study wild-type, fur mutant, and complemented fur mutant strains of the human pathogen Neisseria gonorrhoeae F62 were grown under high (100 uM iron) or low (100 uM desferal) iron conditions to identify genes whose expression was regulated by iron and/or Fur

Organism:

Neisseria gonorrhoeae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20721

10 Samples

Download data: TXT