GEO DataSets

(Submitter supplied) All cows experience bacterial contamination and tissue injury in the uterus postpartum, instigating a local inflammatory immune response. However mechanisms that control inflammation and achieve a physiologically functioning endometrium, while avoiding disease in the postpartum cow are not succinctly defined. This study aimed to identify novel candidate genes indicative of inflammation resolution during involution in healthy beef cows. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11153

6 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15749

50 Samples

Download data

(Submitter supplied) The regulation of endometrial inflammation has important consequences for the resumption of bovine fertility post-partum. All cows experience bacterial influx into the uterus after calving; however a significant proportion fail to clear infection leading to the development of cytological endometritis (CE) and compromised fertility. We hypothesised that early immunological changes could not only act as potential prognostic biomarkers for the subsequent development of disease but also shed light on the pathogenesis of endometritis in the post-partum dairy cow. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15749

20 Samples

Download data: TXT

(Submitter supplied) The regulation of endometrial inflammation has important consequences for the resumption of bovine fertility post-partum. All cows experience bacterial influx into the uterus after calving; however a significant proportion fail to clear infection leading to the development of cytological endometritis (CE) and compromised fertility. We hypothesised that early immunological changes could not only act as potential prognostic biomarkers for the subsequent development of disease but also shed light on the pathogenesis of endometritis in the post-partum dairy cow. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15749

30 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Bos taurus

Type:

Expression profiling by array; Methylation profiling by genome tiling array

Platforms:

GPL11649 GPL16270

48 Samples

Download data: TXT

(Submitter supplied) Post-partum uterine inflammation (endometritis) is associated with lower fertility at both the time of infection and after the inflammation has resolved. It was hypothesized that aberrant DNA methylation may be involved in the sub-fertility associated with post-partum uterine inflammation. The objective of this study was to characterize genome-wide DNA methylation and gene expression in the endometrium of dairy cows with sub-clinical endometritis. more...

Organism:

Bos taurus

Type:

Methylation profiling by genome tiling array

Platform:

GPL16270

24 Samples

Download data: TXT

(Submitter supplied) Post-partum uterine inflammation (endometritis) is associated with lower fertility at both the time of infection and after the inflammation has resolved. It was hypothesized that aberrant DNA methylation may be involved in the sub-fertility associated with post-partum uterine inflammation. The objective of this study was to characterize genome-wide DNA methylation and gene expression in the endometrium of dairy cows with sub-clinical endometritis. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11649

24 Samples

Download data: TXT

(Submitter supplied) We aim at identifying possible differences in miRNA profiles and individual circulating miRNA between healthy cows and cows with persistent inflammation as diagnosed by cytology and histology. Ultimately the miRNA profiles will be correslated with RNAseq results to be performed from endometrial biopsies (planned later on this year following laser micro dissection). Samples have been taken from 6 "healthy cows" with low presence of immune cells at 21 and 44 days post partum which were subsequently pregnant, and from 11 cows with high numbers of immune cells at 21 and 44 days post partum, all non pregnant.

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL23295

34 Samples

Download data: TXT

(Submitter supplied) Background: Physiological inflammation of the uterus postpartum is essential for the reparative processes of involution after calving. In the majority of cows, this inflammation is resolved and homeostasis is restored. However, in a significant subset, inflammation persists and contributes to tissue damage, pathology and subfertility. Transcriptomic differences of immune genes between cattle that resolve inflammation and those that develop uterine disease have been detected as early as 7 days postpartum (DPP) suggesting that the host immune response plays an important role in disease outcome. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19172

30 Samples

Download data: XLSX

(Submitter supplied) Cattle with subclinical endometritis (SCE) are sub-fertile, but there is no predictive or diagnosis tool for subclinical uterine disease. The hypothesis for this study was that endometrial inflammation is reflected in peripheral blood leucocytes gene expression. Transcriptome patterns in healthy cows and in cows with SCE at 45-55 days postpartum were evaluated using circulating white blood cells and endometrial biopsies samples collected from the same animals. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL25177

16 Samples

Download data: TXT

(Submitter supplied) The objective of this study was to evaluate the association between progesterone concentration (P4) at day 4 of the estrous cycle (beginning of diestrus) and endometrial global gene expression at day 9 (middle diestrus) in lactating grazing dairy cows. Blood samples were obtained on days 0, 4 and 9 for P4 measurement by chemiluminescence. Cows were asignated to one of the following groups (n=3/group): cows with low physiological P4 at day 4 (PLP4), cows in anestrous (ANE), cows with high physiological P4 at day 4 (HPP4) and superovulated cows (SO). more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22316

12 Samples

Download data: TXT

(Submitter supplied) Pregnancy induces changes in the transcriptome of the bovine endometrium from 15 days after insemination. However, pregnancy is less likely to occur if cows had a postpartum bacterial infection of the uterus. We hypothesized that uterine bacterial infection alters the endometrial transcriptomic signature of pregnancy. To examine the endometrial transcriptomic signature of pregnancy, cows were inseminated 130 days after intrauterine infusion of pathogenic bacteria and endometrium was collected 16 days later for RNA sequencing. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26012

7 Samples

Download data: XLS

(Submitter supplied) The study investigated gene expression patterns in liver tissue to understand the biological functions of genes that are potentially involved in controlling / regulating feed efficiency in Nordic dairy cattle.

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19172

38 Samples

Download data: TXT

(Submitter supplied) Most dairy cows suffer uterine microbial contamination postpartum. Persistent endometritis often develops, associated with reduced fertility. We used a model of differential feeding and milking regimes to produce cows in differing negative energy balance (NEB) status in early lactation. We used Affymetrix GeneChipÒ Bovine Genome Array to investigate the global gene expression underlying negative energy balance and to identify the significantly differentially expressed genes during this process.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

11 Samples

Download data: CEL

(Submitter supplied) We investigated differential gene expression profiles of endometrium during the mid-luteal phase of the estrous cycle between repeat breeding (RB) and normally fertilized cows using microarray analysis. Caruncular (CAR) and intercaruncular (ICAR) endometrium of both ipsilateral and contralateral uterine horns to the corpus luteum were collected from RB (inseminated at least three times but not pregnant) and normally fertile cows on Day 15 of the estrous cycle. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL19801

32 Samples

Download data: TXT

(Submitter supplied) We evaluated liver transcriptome throughout gestation and early lactation of purebred and crossbred beef cows (Angus, Hereford and their F1 crossbreeds, CR), grazing high or low herbage allowances (HA) of native grasslands (4 and 2.5 kg DM/kg BW annual mean; n = 16) using an Agilent 4x44k bovine array. A total of 4,661 transcripts were affected by days (272 ≥ 2.5-fold difference, FDR ≤ 0.10) and 47 pathways were altered during winter gestation (-165 to -15 days relative to calving, d). more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11648

64 Samples

Download data: TXT

(Submitter supplied) We hypothesized that the uterine microbiome postpartum would affect the endometrial transcriptome in “real-time” (implicating a direct interaction between microbial products and the endometrium and also that the microbiome could program the future function of the endometrium (implicating a uterine programming perhaps through mechanisms similar to inflammatory memory). To test this hypothesis, we performed 16S rRNA gene sequencing of the endometrial microbiome at one, five and nine weeks after calving and tested for an effect of the microbial population on the endometrial transcriptome at five and nine weeks after calving using mRNA-sequencing. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23055

50 Samples

Download data: TXT

(Submitter supplied) We examined the molecular profile of human endometrium on days 2, 3 and 4 of menstruation to identify genes and pathways that play a role in the menstrual process. Endometrial samples were collected by Pipelle biopsy on days 2 (n=9), 3 (n=9) or 4 (n=6) of menstruation and arrayed. We identified key gene pathways involved in menstruation and identified a number of genes not previously associated with the process (Paiva et al. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL22366

24 Samples

Download data: TSV

(Submitter supplied) In dairy cows, administration of high dosages of niacin (NA) was found to cause anti-lipolytic effects, which are mediated by the NA receptor hydroxyl-carboxylic acid receptor 2 (HCAR2) in white adipose tissue (WAT), and thereby to an altered hepatic lipid metabolism. However, almost no attention has been paid to possible direct effects of NA in cattle liver, despite showing that HCAR2 is expressed also in the liver of cattle and is even more abundant than in WAT. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL16500

18 Samples

Download data: CEL

(Submitter supplied) Background Bovine Spastic Paresis (BSP) is a neuromuscular disorder which affects both male and female cattle. BSP is characterized by spastic contraction and overextension of the gastrocnemious muscle of one or both limbs and is associated with a scarce increase in body weight. This disease seems to be caused by an autosomal and recessive gene, with incomplete penetration, although no genes clearly involved with its onset have been so far identified. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL11188

2 Samples

Download data: MEV