GEO DataSets

(Submitter supplied) Hxt1 is a high affinity hexose transporter important for virulence of the phytopathogenic basidiomycete Ustilago maydis on its host plant maize. Hxt1 shows the highest similarities to the glucose sensors Rgt2 and Snf3 from S. cereviciae (42% and 39% identity on amino acid level, respectively). In these sensors, the substitution of a highly conserved arginine to lysine leads to a constitutive active signal, resulting in expression of several glucose induced genes. more...

Organism:

Mycosarcoma maydis

Type:

Expression profiling by array

Platform:

GPL3681

9 Samples

Download data: CEL

(Submitter supplied) Ustilago maydis is a plant-pathogenic fungus that establishes a biotrophic relationship with its host Zea mays. The biotrophic interaction is initiated upon host penetration, and involves expansion of the host plasma membrane around hyphae, which is thought to facilitate the exchange of nutrients and virulence factors. Transcriptional regulators involved in the establishment of an infectious dikaryon and penetration into the host have been identified, however, regulators involved in the post-penetration stages remained to be elucidated. more...

Organism:

Zea mays

Type:

Expression profiling by array

Platform:

GPL4032

9 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mycosarcoma maydis

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19655

18 Samples

Download data

(Submitter supplied) Goals: characterization of the transcription factor Ros1 in Ustilago maydis Methods: generation of deletion mutants, microscopic observations, ectopic expression of ros1, identification of Ros1 regulated genes by RNAseq and ChIP sequencing Results: Ros1 is not involved in plant colonization but is essential to trigger sporogenesis during late stages of infection. Premature expression of ros1 revealed that Ros1 counteracts the b-dependent filamentation program and induces morphological alterations resembling the early steps of sporogenesis. more...

Organism:

Mycosarcoma maydis

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19655

12 Samples

Download data: CSV

(Submitter supplied) Goals: characterization of the transcription factor Ros1 in Ustilago maydis Methods: generation of deletion mutants, microscopic observations, ectopic expression of ros1, identification of Ros1 regulated genes by RNAseq and ChIP sequencing Results: Ros1 is not involved in plant colonization but is essential to trigger sporogenesis during late stages of infection. Premature expression of ros1 revealed that Ros1 counteracts the b-dependent filamentation program and induces morphological alterations resembling the early steps of sporogenesis. more...

Organism:

Mycosarcoma maydis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19655

6 Samples

Download data: CSV, TXT

(Submitter supplied) We show that combinations of organic acids and glucose trigger phenotypes associated with the late stage of biotrophy for the maize pathogen Ustilago maydis. These phenotypes include the expression of a set of effectors normally observed only during biotrophic development as well as the formation of melanin associated with sporulation in plant tumors.

Organism:

Mycosarcoma maydis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL31263

18 Samples

Download data: CSV

(Submitter supplied) The basidiomycete Ustilago maydis causes smut disease in maize. Colonization of the host plant is initiated by direct penetration of cuticle and cell wall of maize epidermis cells. The invading hyphae are surrounded by the plant plasma membrane and proliferate within the plant tissue. We identified a novel secreted protein, termed Pep1. Disruption mutants of pep1 are not affected in saprophytic growth and develop normal infection structures. more...

Organism:

Zea mays

Type:

Expression profiling by array

Platform:

GPL4032

6 Samples

Download data: CEL, CHP

(Submitter supplied) The basidiomycete fungus Ustilago maydis causes smut disease in maize and has become an important model for elucidating the strategies used for host colonization by biotrophic fungi. In this study, we performed an in-depth transcriptional profiling of the plant-associated development of a cross between U. maydis FB1 and FB2 wildtype strains. The analysis of eight different stages, including the development on the leaf surface, early colonization, tumor induction and spore maturation, offers an unprecedented view of the changes in the fungal transcriptome associated with the passage through the entirely biotrophic life cycle. more...

Organism:

Zea mays; Mycosarcoma maydis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL19655 GPL24015 GPL15463

45 Samples

Download data: TXT

(Submitter supplied) Anthocyanin induction in plant is considered a general defense response against biotic and abiotic stresses. The infection by Ustilago maydis, the corn smut pathogen, is accompanied with anthocyanin induction in leaf tissue. We revealed that anthocyanin is intentionally induced by the virulence promoting secreted effector protein Tin2. Tin2 protein functions inside plant cells where it interacts with cytoplasmic maize protein kinase ZmTTK1. more...

Organism:

Zea mays

Type:

Expression profiling by array

Platform:

GPL4032

9 Samples

Download data: CEL

(Submitter supplied) The phenolic compound salicylic acid (SA) is a key signaling molecule regulating local and systemic plant defense responses against biotrophs. Many microbial organisms, including pathogens, share the ability to degrade SA. However, the mechanism by which they perceive SA is unknown. We showed that Ustilago maydis, the causal agent of corn smut disease, employs a so far uncharacterized SA sensing mechanism. more...

Organism:

Zea mays; Mycosarcoma maydis

Type:

Expression profiling by array

Platform:

GPL22065

6 Samples

Download data: TXT

(Submitter supplied) The fungus Ustilago maydis is a biotrophic pathogen of corn. In its genome we have identified an ortholog of YAP1 from Saccharomyces cerevisae which regulates the oxidative stress response in this organism. yap1 mutants of U. maydis displayed higher sensitivity to H2O2 than wild type cells and their virulence was significantly reduced. U. maydis yap1 could partially complement the H2O2 sensitivity of a yap1 deletion mutant of S. more...

Organism:

Mycosarcoma maydis

Type:

Expression profiling by array

Platform:

GPL3681

6 Samples

Download data: CEL

(Submitter supplied) Ustilago maydis is a basidiomycete fungus that causes smut disease in maize. Most prominent symptoms of the disease are plant tumors, which can be induced by U. maydis on all aerial parts of the plant. We identified two linked genes, pit1 and pit2, which are specifically expressed during plant colonization. Deletion mutants for either pit1 or pit2 are unable to induce tumor development and elicit plant defense responses. more...

Organism:

Zea mays

Type:

Expression profiling by array

Platform:

GPL4032

6 Samples

Download data: CEL, CHP

(Submitter supplied) The clp1 gene is a b-dependent gene required for clamp formation during the biotrophic growth phase of U. maydis. The gene was induced by means of a fusion to the arabinose inducable crg1 promoter. To exclude effects from the b-mating type locus, the experiments were performed in strain JB1 carrying a deletion of the entire b-locus. Keywords: gene induction

Organism:

Mycosarcoma maydis

Type:

Expression profiling by array

Platform:

GPL3681

4 Samples

Download data

(Submitter supplied) We use this array to determine genes under the control of the transcriptional regulator Tup1

Organism:

Mycosarcoma maydis

Type:

Expression profiling by array

Platform:

GPL3681

4 Samples

Download data: CEL

(Submitter supplied) Adr1 of Ustilago maydis is a protein kinase that is activated after separation from the regulatory subunit mediated by high cAMP levels. A copy of the gene under the control of the arabinose-inducible crg1 promotor was introduced into the Cbx locus of the wild type strain FB1 creating strain HE140. To monitor the transcriptional changes upon adr1 induction by arabinose, we conducted a time course experiment comparing the wild type transcriptional response before and 75 or 180 min after medium shift to complete medium containing arabinose, as well as the response of the mutant HE140 before and 75 or 180 min after induction of the additional copy of adr1 by arabinose. more...

Organism:

Mycosarcoma maydis

Type:

Expression profiling by array

Platform:

GPL3681

16 Samples

Download data: CEL

(Submitter supplied) Urbs1 of Ustilago maydis is a transcription factor that has been shown to repress its target genes (e.g. sid1, sid2) in the presence of iron. To identify additional genes regulated by Urbs1, we compared transcriptional profiles of the Ustilago maydis wild type strain FB1 grown in complete medium containing glucose with the addition of 10 µM FeSO4 to the Ustilago maydis urbs1 deletion mutant BW12 grown under the same conditions. more...

Organism:

Mycosarcoma maydis

Type:

Expression profiling by array

Platform:

GPL3681

4 Samples

Download data: CEL

(Submitter supplied) Identification of transcriptional changes of Ustilago maydis wild type strain FB1 grown in axenic culture in complete medium containing glucose without or with the addition of 10 µM FeSO4. Keywords: gene induction

Organism:

Mycosarcoma maydis

Type:

Expression profiling by array

Platform:

GPL3681

4 Samples

Download data: CEL

(Submitter supplied) Two-organism transcriptome profiling of infected seedling, adult leaf, and tassel demonstrated that both the host and pathogen exhibit organ-specific expression programs. Phenotypic screening of U. maydis mutants deleted for suites of secreted protein genes and maize growth mutants demonstrated organ-restricted tumorigenesis. Two-dye, competitive hybridizations were performed on Agilent oligo arrays. more...

Organism:

Zea mays; Mycosarcoma maydis

Type:

Expression profiling by array

Platform:

GPL9997

48 Samples

Download data: TXT

(Submitter supplied) Transcriptomes from multiple pre-meiotic stages of wild type, mac1, and msca1 maize anthers were characterized by microarray hybridization. The goal was to characterize the developmental progression as the anther specifies five cell types and grows rapidly precedeing meiotic entry. The stages characterized were immature anther primordia (0.15 mm long in maize) containing just stem cells, through somatic and germinal cell fate specification (0.20 and 0.25 mm), mitotic proliferation (0.4 mm), and finally the birth of the middle layer and tapetum (0.7 mm). more...

Organism:

Zea mays

Type:

Expression profiling by array

Platform:

GPL7444

48 Samples

Download data: TXT

(Submitter supplied) Two-organism transcriptome profiling of infected maize anthers demonstrated that there are anther-specific gene expression programs for both the pathogen and the host. Two-dye, competitive microarray hybridizations were performed on Agilent oligo arrays. Confocal microscopy on other anthers demonstrated that U. maydis was in contact with all anther lobe cell types by 3 days post-infection and that the pathogen disrupted both host cell division and cell expansion patterns.

Organism:

Zea mays; Mycosarcoma maydis

Type:

Expression profiling by array

Platform:

GPL9997

8 Samples

Download data: TXT