Similar studies for GEO DataSets (Select 200067123) - GEO DataSets

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Items: 20

Select item 2000671231.

Tracing the formation of hematopoietic stem cells in mouse embryos by single-cell functional and RNA-Seq analyses

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platforms:: GPL13112 GPL17021
216 Samples

Download data: FPKM_TRACKING

Series

Accession:: GSE67123

ID:: 200067123

PubMed Similar studies

Select item 2000671202.

Tracing the Formation of Haematopoietic Stem Cells in Mouse Embryos by Single-cell Functional and RNA-Seq Analyses [single-cell]

(Submitter supplied) Haematopoietic stem cells (HSCs) are derived early from embryonic precursor cells, such as haemogenic endothelial cells and pre-HSCs. However, the identity of precursor cells remains elusive due to their rareness, transience, and inability to be isolated efficiently. Here we employed potent surface markers to capture the nascent pre-HSCs at 30% purity, as rigorously validated by single-cell-initiated serial transplantation assay. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platforms:: GPL13112 GPL17021
181 Samples

Download data: FPKM_TRACKING, TXT

Series

Accession:: GSE67120

ID:: 200067120

PubMed Similar studies SRA Run Selector

Select item 2000669543.

Tracing the formation of hematopoietic stem cells in mouse embryos by single-cell functional and RNA-Seq analyses [10-cell]

(Submitter supplied) Hematopoietic stem cells (HSCs) in adult are specified early from the endothelium-derived precursors (e.g., hemogenic endothelium, and pre-HSCs) in mouse mid-gestation embryos, the detailed process, however, is still largely unknown due to their rareness, transience, and current inability to prospectively isolate them efficiently . Here we developed a potent set of surface markers that could capture the earliest emerging HSCs, the CD45- pre-HSCs with high accuracy and purity, as rigorously and functionally verified by single-cell-initiated serial transplantation assays. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platforms:: GPL13112 GPL17021
35 Samples

Download data: FPKM_TRACKING

Series

Accession:: GSE66954

ID:: 200066954

PubMed Similar studies SRA Run Selector

Select item 2001629504.

Mapping Human Hematopoietic Stem Cells From Hemogenic Endothelium To Birth

(Submitter supplied) Human hematopoietic stem cell (HSC) ontogeny is poorly defined due to the inability to identify HSCs as they emerge and mature in different hematopoietic sites. We created a single-cell transcriptome map of human hematopoietic tissues from 1st trimester to birth and found that HSC signature RUNX1+HOXA9+MLLT3+MECOM+HLF+SPINK2+ distinguishes HSCs from progenitors throughout gestation. In addition to the AGM (aorta-gonad-mesonephros) region, nascent HSCs populated the placenta and yolk sac before colonizing the liver at 6 weeks. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL24676
28 Samples

Download data: CSV, PNG, TAR

Series

Accession:: GSE162950

ID:: 200162950

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001900115.

Transcriptome analysis of nascent hematopoietic stem cells (HSCs) induced by the ectopic expression of Evi1

(Submitter supplied) To examine the role of Evi1 in HSC specification, we generated Tie2-Cre::ROSA-Evi1 mice, which can induce Evi1 in endothelial cells. We observed five-fold increase of HSCs In Tie2-Cre::ROSA-Evi1 embryo. To characterize the induced HSCs in Tie2-Cre::ROSA-Evi1 embryos, we analyzed the gene expression profile of HSCs.

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL19057
9 Samples

Download data: TXT

Series

Accession:: GSE190011

ID:: 200190011

PubMed Similar studies

Select item 2001680546.

Transcriptome analysis of nascent hematopoietic cells II

(Submitter supplied) During ontogeny, HSCs or progenitors are generated from endothelial cells through the process known as endothelial-to-hematopoietic transition (EHT). After EHT, hematopoietic cells form cell aggregates, called hematopoietic clusters. To obtain mechanistic insight into HSC specification, we compared the gene expression profiles of hematopoietic clusters between caudal half region and yolk sac.

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL19057
6 Samples

Download data: TXT

Series

Accession:: GSE168054

ID:: 200168054

PubMed Similar studies SRA Run Selector

Select item 2001679327.

The precursors of hematopoietic stem cells during mouse embryogenesis

(Submitter supplied) Definitive hematopoietic stem cells (HSCs) bud off from the hemogenic endothelial cells (HEC) located in the dorsal aorta of the mouse embryo. The maturation of HSCs from HEC occurs through the precursor of HSCs (pre-HSCs) between embryonic day (E) 9.5 and E11.5. To clarify the differentiation process of pre-HSCs, we performed single-cell RNA-seq analysis of cells dissociated from the dorsal aorta and its surrounding tissues and the fetal liver at E10.5 and E11.5. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL21273
2 Samples

Download data: MTX, TSV

Series

Accession:: GSE167932

ID:: 200167932

PubMed Similar studies SRA Run Selector

Select item 2000888408.

Conversion of adult endothelium to immunocompetent haematopoietic stem cells

(Submitter supplied) Developmental pathways that orchestrate the fleeting transition of endothelial cells into haematopoietic stem cells remain undefined. Here we demonstrate a tractable approach for fully reprogramming adult mouse endothelial cells to haematopoietic stem cells (rEC-HSCs) through transient expression of the transcription-factor-encoding genes Fosb, Gfi1, Runx1, and Spi1 (collectively denoted hereafter as FGRS) and vascular-niche-derived angiocrine factors. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL17021
30 Samples

Download data: TSV

Series

Accession:: GSE88840

ID:: 200088840

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001165309.

Differentiation of transplanted haematopoietic stem cells tracked by single-cell transcriptomic analysis

(Submitter supplied) Transcriptome analysis of hematopoietic cells under homeostasis and regeneration at single cell resolusion

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL21103
54 Samples

Download data: TXT, XLS

Series

Accession:: GSE116530

ID:: 200116530

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20011264210.

Single cell transcriptomics reveal the dynamic of haematopoietic stem cell production in the aorta

(Submitter supplied) We present single-cell mRNA-Sequencing of various endothelial and hematopoietic populations isolated from the mouse embryonic aorta at E10 and E11. Our study reveals the transcriptional dynamics occuring during endothelial to hematopoietic transition, the process responsible for the production of hematopoietic stem cells.

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platforms:: GPL13112 GPL19057
14 Samples

Download data: CSV

Series

Accession:: GSE112642

ID:: 200112642

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20005725111.

Gfi1 proteins regulate the emergence of HSCs and blood precursors in the AGM through recruitment of LSD1

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Expression profiling by array; Other

Platforms:: GPL6193 GPL17021
5 Samples

Download data: CEL

Series

Accession:: GSE57251

ID:: 200057251

Select item 20005724812.

Gfi1 proteins regulate the emergence of HSCs and blood precursors in the AGM through recruitment of LSD1 [HTS]

(Submitter supplied) Gfi1:Dam and Gfi1b:Dam binding analysis of sorted hemogenic endothelial cells (TIE2/C-KIT)

Organism:: Mus musculus

Type:: Other

Platform:: GPL17021
3 Samples

Download data: XLSX

Series

Accession:: GSE57248

ID:: 200057248

PubMed Similar studies SRA Run Selector

Select item 20005720813.

Gfi1 proteins regulate the emergence of HSCs and blood precursors in the AGM through recruitment of LSD1 [Affymetrix]

(Submitter supplied) Transcriptome analysis of sorted hemogenic endothelial cells with and without Lsd1 inhibition with 300nM of compound B. Transcriptome analysis to detect the global changes in gene expression upon LSD1 inhibition.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6193
2 Samples

Download data: CEL

Series

Accession:: GSE57208

ID:: 200057208

Select item 20008941814.

EZH1 as a key epigenetic barrier to definitive haematopoiesis during embryonic development

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens; Mus musculus

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

4 related Platforms
58 Samples

Download data: BED, TXT, WIG

Series

Accession:: GSE89418

ID:: 200089418

PubMed Full text in PMC Similar studies

Select item 20008941715.

ChIP-seq analysis of EZH1, H3K4me3 and H3K27me3 in 5F cells

(Submitter supplied) Blood develops in distinct stages. Haematopoietic progenitors in the embryo manifest restricted differentiation potential relative to definitive haematopoietic stem cells in adult bone marrow, which support lifelong multilineage haematopoiesis. To identify regulators of embryonic haematopoiesis, we screened chromatin modifiers and identified the Polycomb group protein EZH1 as a barrier to multilineage potential from pluripotent stem cells (PSCs). more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL18573
15 Samples

Download data: BED, WIG

Series

Accession:: GSE89417

ID:: 200089417

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20008941616.

ATAC-seq analysis in 5F cells and AGM cells with EZH1 depletion

Organism:: Homo sapiens; Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platforms:: GPL19057 GPL18573
7 Samples

Download data: BED, WIG

Series

Accession:: GSE89416

ID:: 200089416

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20008941517.

RNA-seq analysis of EZH1 knockdown in 5F cells, or EZH1 heterozygous and homozygous knockout YS and AGM cells

Organism:: Homo sapiens; Mus musculus

Type:: Expression profiling by high throughput sequencing

Platforms:: GPL17021 GPL16791
36 Samples

Download data: TXT

Series

Accession:: GSE89415

ID:: 200089415

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20011446418.

Mesoderm-derived PDGFRA+ cells regulate emergence of hematopoietic stem cells in the dorsal aorta

(Submitter supplied) During embryonic development, the first hematopoietic stem cells (HSCs) arise from a transient population of endothelial cells lining the ventral surface of the dorsal aorta, via a process of endothelial to hematopoietic transition (EHT) at embryonic day (E) 10.5. This region contains resident PDGFRA+ stromal cells (PSCs), but their identity and role in HSC generation in the AGM are not well understood. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL21273
27 Samples

Download data: BW

Series

Accession:: GSE114464

ID:: 200114464

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20005766219.

Reprogramming of Endothelium Into Hematopoietic Progenitors by Defined Factors and Vascular Induction

(Submitter supplied) Generation of abundant engraftable hematopoietic cells from autologous tissues promises new therapies for hematologic diseases. Differentiation of pluripotent stem cells into hematopoietic cells results in emergence of cells that have poor engraftment potential. To circumvent this hurdle, we have devised a vascular niche model to phenocopy the developmental microenvironment of hemogenic cells thereby enabling direct transcriptional reprogramming of human endothelial cells (ECs) into hematopoietic cells. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL11154
16 Samples

Download data: TXT

Series

Accession:: GSE57662

ID:: 200057662

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 20004502020.

Retinoic acid signaling is essential for HSC development

(Submitter supplied) To find signaling regulators that modulate transition from endothelial (AA4.1/VEC+CD45-) to hemogenic endothelial (AA4.1/VEC+ CD45+) cell

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6887
6 Samples

Download data: TXT

Series

Accession:: GSE45020

ID:: 200045020

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