GEO DataSets

(Submitter supplied) Drosophila sechellia relies exclusively on the fruits of Morinda citrifolia, which are toxic to most insects, including its sibling species D. melanogaster and D. simulans. Although several odorant binding protein (Obp) genes and olfactory receptor (Or) genes were suggested to be associated with the D. sechellia host shift, a broad view of how chemosensory genes have contributed to this shift is still lacking. more...

Organism:

Drosophila sechellia

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20000

6 Samples

Download data: FPKM_TRACKING

(Submitter supplied) Background Antennae of fruit flies are the major organs responsible for detecting environmental volatiles, e.g., egg-laying substrates. An adult antenna contains many sensilla full of olfactory sensory neurons, where olfactory receptor (Or) genes are expressed. Each sensory neuron only expresses up to three receptors, making it difficult to estimate expression levels by conventional methods. In this study, we applied Illumina RNA sequencing (RNA-seq) to study the expression levels of Or and other genes in fly antennae. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13304

2 Samples

Download data: FPKM_TRACKING

(Submitter supplied) Drosophila simulans relies exclusively on the fruits of Morinda citrifolia, which are toxic to most insects, including its sibling species D. melanogaster and D. simulans. Although several odorant binding protein (Obp) genes and olfactory receptor (Or) genes were suggested to be associated with the D. simulans host shift, a broad view of how chemosensory genes have contributed to this shift is still lacking. more...

Organism:

Drosophila simulans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13306

6 Samples

Download data: FPKM_TRACKING

(Submitter supplied) Drosophila sechellia relies exclusively on the fruits of Morinda citrifolia, which are toxic to most insects, including its sibling species D. melanogaster and D. simulans. Although several odorant binding protein (Obp) genes and olfactory receptor (Or) genes were suggested to be associated with the D. sechellia host shift, a broad view of how chemosensory genes have contributed to this shift is still lacking. more...

Organism:

Drosophila sechellia

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20000

6 Samples

Download data: FPKM_TRACKING

(Submitter supplied) Here we show that Drosophila sechellia—a specialist on the fruit of Morinda citrifolia that recently diverged from its generalist sister-species, D. simulans—has rapidly accumulated loss-of-function alleles and reduced gene expression at genes affecting olfaction, detoxification, and metabolism. While D. sechellia increases expression of genes involved with oogenesis and fatty acid metabolism when on its host, many more genes show reduced expression in D. more...

Organism:

Drosophila melanogaster; Drosophila simulans; Drosophila sechellia

Type:

Expression profiling by array

Platforms:

GPL3830 GPL3829

20 Samples

Download data: TXT

(Submitter supplied) Here we show that Drosophila sechellia—a specialist on the fruit of Morinda citrifolia that recently diverged from its generalist sister-species, D. simulans—has rapidly accumulated loss-of-function alleles and reduced gene expression at genes affecting olfaction, detoxification, and metabolism. While D. sechellia increases expression of genes involved with oogenesis and fatty acid metabolism when on its host, many more genes show reduced expression in D. more...

Organism:

Drosophila sechellia; Drosophila simulans; Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL3829

24 Samples

Download data: TXT

(Submitter supplied) Here we show that Drosophila sechellia—a specialist on the fruit of Morinda citrifolia that recently diverged from its generalist sister-species, D. simulans—has rapidly accumulated loss-of-function alleles and reduced gene expression at genes affecting olfaction, detoxification, and metabolism. While D. sechellia increases expression of genes involved with oogenesis and fatty acid metabolism when on its host, many more genes show reduced expression in D. more...

Organism:

Drosophila melanogaster; Drosophila sechellia; Drosophila simulans

Type:

Expression profiling by array

Platform:

GPL1322

8 Samples

Download data: CEL

(Submitter supplied) The dietary specialist fruit fly Drosophila sechellia has evolved to specialize on the toxic fruit of its host plant Morinda citrifolia. Toxicity of Morinda fruit is primarily due to high levels of octanoic acid (OA). Using RNA interference (RNAi), prior work found that knockdown of Osiris family genes Osiris 6 (Osi6), Osi7, and Osi8 led to increased susceptibility to OA in adult D. melanogaster flies, likely representing genes underlying a Quantitative Trait Locus (QTL) for OA resistance in D. more...

Organism:

Drosophila sechellia

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24063

6 Samples

Download data: TXT

(Submitter supplied) We analyze the gene expression response to hexanoic acid in Drosophila simulans, Drosophila melanogaster, and Drosophila sechellia

Organism:

Drosophila melanogaster; Drosophila sechellia; Drosophila simulans

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24063 GPL27544 GPL21306

18 Samples

Download data: TXT

(Submitter supplied) Chemoreception is based on the senses of smell and taste that are crucial for animals to find new food sources, shelter, and mates. The initial step in olfaction involves the translocation of odorants from the periphery through the aqueous lymph of the olfactory sensilla to the odorant receptors by chemosensory proteins (CSPs) or odorant binding proteins (OBPs).To better understand the roles of CSPs and OBPs in a coleopteran pest species, the red flour beetle Tribolium castaneum (Coleoptera, Tenebrionidae), we performed transcriptome analyses of male and female antennae, heads, mouthparts, legs, and bodies, which revealed that all 20 CSPs and 49 of the 50 previously annotated OBPs are transcribed. more...

Organism:

Tribolium castaneum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18255

16 Samples

Download data: FA, TXT

(Submitter supplied) The dietary specialist fruit fly Drosophila sechellia evolved to feed primarily on the toxic fruit of Morinda citrifolia. Seeking the basis of adaptations to the unique chemistry of its host plant, we profiled genome-wide gene expression response to L-DOPA because it is highly abundant in M. citrifolia, critical for reproductive success of D. sechellia, and may influence toxin resistance in this species. more...

Organism:

Drosophila sechellia; Drosophila simulans; Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL21306 GPL24063 GPL27544

18 Samples

Download data: TXT

(Submitter supplied) Drosophila sechellia is an island endemic host specialist that has evolved to consume the toxic fruit of Morinda citrifolia, also known as noni fruit. Recent studies by our group and others have examined genome-wide gene expression responses of fruit flies to individual highly abundant compounds found in noni responsible for the fruit’s unique chemistry and toxicity. In order to relate these reductionist experiments to the gene expression responses to feeding on noni fruit itself, we fed rotten noni fruit to adult female D. more...

Organism:

Drosophila sechellia

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24063

6 Samples

Download data: CSV

(Submitter supplied) The underlying genetic basis of adaptive phenotypic changes are generally poorly understood, yet a growing number of case studies are beginning to shed light on important questions about the molecular nature and pleiotropy of such changes. We use Drosophila sechellia, a dietary specialist fruit fly that has evolved to specialize on a single toxic host plant: Morinda citrifolia, as a model for adaptive phenotypic change and seek to determine the genetic basis of traits associated with host specialization in this species. more...

Organism:

Drosophila sechellia

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24063

6 Samples

Download data: TXT

(Submitter supplied) Deep sequencing of total RNA extracted from the genital discs of males for each of the following strains : Drosophila sechellia, Drosophila mauritiana, hybrid introgression line 3Q1(A) and hybrid introgression line Q1(A)

Organism:

Drosophila sechellia x Drosophila mauritiana; Drosophila sechellia; Drosophila mauritiana

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL13416 GPL10071 GPL13417

4 Samples

Download data: SAM, TXT

(Submitter supplied) We performed genome-wide expression assays comparing gene expression in the Drosophila melanogaster third larval instar genital imaginal disc between males and females. We used microarrays to compare the relative expression levels of five independent male versus female comparisons for each of two different D. melanogaster wild-type strains, Canton-S and Berlin.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL6799

10 Samples

Download data: GPR

(Submitter supplied) Large multigene families, such as the insect odorant binding proteins (OBPs), are thought to arise through functional diversification after repeated gene duplications. Whereas many OBPs function in chemoreception, members of this family are also expressed in tissues outside chemosensory organs. Paralogs of the Obp50 gene cluster are expressed in metabolic and male reproductive tissues, but their functions and interrelationships remain unknown. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25244

56 Samples

Download data: TXT

(Submitter supplied) These data were generated in a study to analyze the genetic causes of gene regulatory divergence. mRNA-seq libraies were prepared from poly(A)+ RNA prepared from whole F1 hybrids of D. melanogaster and D. sechellia (female 0-3d post eclosion, "D_mel_x_D_sec"). A mixed mRNA- seq library was prepared from D. melanogaster and D. sechellia poly(A) + RNA (mixed before library preparation, "D_mel_+_D_sec). more...

Organism:

Drosophila melanogaster; Drosophila melanogaster x Drosophila sechellia; Drosophila sechellia

Type:

Expression profiling by high throughput sequencing

4 related Platforms

4 Samples

Download data: BED

(Submitter supplied) Ecdysone-induced protein 93F (E93) regulates local chromatin structure and/or gene transcription, raising the question of whether expression of Ir75c, Ir75b and Ir75a receptors in antenna is affected at the transcriptional level in the absence of this protein. We performed bulk RNA-sequencing (RNA-seq) of control and E93RNAi antennae. In control RNA-seq samples, we predicted the existence of seven distinct transcripts encoded by these three receptor genes. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17275

6 Samples

Download data: TXT

(Submitter supplied) We performed mRNA-seq of dissected Drosophila mushroom bodies, comparing to whole brain and testis

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL9058

4 Samples

Download data

(Submitter supplied) Young, or newly evolved, genes arise ubiquitously across the tree of life, and can rapidly acquire novel functions that influence a diverse array of biological processes (Chen et al. 2013)1. Previous work identified a young regulatory gene in Drosophila, Zeus, which diverged rapidly from its parent Caf40 and took on roles in the male reproductive system. This neofunctionalization was accompanied by differential binding of the Zeus protein to loci throughout the Drosophila melanogaster genome Chen et al. more...

Organism:

Drosophila melanogaster

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17275

13 Samples

Download data: BED