GEO DataSets

(Submitter supplied) Upon 2-cell embryo splitting, individual blastomeres were cultured in KSOM(aa) medium and developed into blastocysts, which were compared and contrasted with each other respecting pair associations (e.g. twin vs cotwin) Transcriptome analysis revelaled minimal differences between twins and unmanipulated controls, in contrast to larger differences between twins and cotwins; these differences are mainly related to the epiblast lineage

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL20775

25 Samples

Download data: CEL, TXT

(Submitter supplied) Upon 2-cell embryo splitting, individual blastomeres were compared and contrasted with each other respecting pair associations (e.g. blastomere '1a' and '1b' of embryo 1, '2a' and '2b' of embryo 2, and so forth) Transcriptome analysis followed by cluster analysis (Ward) was able to match a minority of the blastomeres with the correct sister blastomere

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL20775

20 Samples

Download data: CEL, TXT

(Submitter supplied) In multicellular organisms, heterogametes of oocytes and sperms are fertilized and resulting zygotes give rise to new individuals. The ability of zygotes that produce a fully formed individual from single cell when placed in a supportive environment is defined as totipotency. Given that totipotent cells are the source of all multicellular organisms, better understanding of totipotency has a profound effect on not only biology but also our society. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18480

2 Samples

Download data: CSV, TXT

(Submitter supplied) Mouse zygotes are suspected to mount adaptive responses (e.g. physiology, metabolism, gene expression) to the environment, which can be the natural environment of the female genital tract or the artificial environment of a culture medium. The jury is still out when it comes to the possible long-term effects of culture media on embryo development. In 2016 the ESHRE took a stance about this subject, saying that the environment the early embryo is exposed to can cause reprogramming of embryonic, fetal and postnatal development, with negative consequences for the conceptus (Sunde et al., Hum Reprod. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL20258

36 Samples

Download data: CEL, XLSX

(Submitter supplied) Following fertilization in mammals, it is generally accepted that totipotent cells are exclusive to the zygote and to each of the two blastomeres originating from the first mitotic division. We counter that this classic view needs to be revised, because we have presented compelling evidence that the sister blastomeres are both totipotent in only a subset of 2-cell stage mouse embryos (PMID 28811525). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL20775

18 Samples

Download data: CEL

(Submitter supplied) Among the different procedures of the assisted reproductive technologies (ARTs), the purpose of ovarian stimulation with gonadotropins is to maximize the number of oocytes retrieved, and facilitate scheduling and planning of patient treatment. However, the doses of the gonadotropins required to increase the number of growing ovarian follicles, and, thereby, the number of oocytes, have been found to affect the quality of embryos in the mouse model of human reproduction (PMID 16569317, PMID 22802074, PMID 11387298, PMID 11157810). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL20775

16 Samples

Download data: CEL

(Submitter supplied) The aberrant gene expression of early bovine embryos are a major cause for developemtal arrest. Therefore our aim was to detect transcriptomic fingerprints which correlate with the subsequent developmental competence of a two cell stage embryo.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

12 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL20795

112 Samples

Download data: BW, NARROWPEAK, TXT

(Submitter supplied) Supplemented with splicing inhibitor PlaB, we realize in vitro culturing of human totipotent stem cells comparable to 2-4-cell blastomeres Assay for transposase accessible chromatin with high-throughput sequencing in hESC sand hTBLCs

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL20795

2 Samples

Download data: NARROWPEAK

(Submitter supplied) Supplemented with splicing inhibitor PlaB, we realize in vitro culturing of human totipotent stem cells comparable to 2-4-cell blastomeres

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20795

14 Samples

Download data: TXT

(Submitter supplied) Supplemented with splicing inhibitor PlaB, we realize in vitro culturing of human totipotent stem cells comparable to 2-4-cell blastomeres Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for h3k4me3 and h3k27me3 in hESC sand hTBLCs

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL20795

4 Samples

Download data: BW

(Submitter supplied) Supplemented with splicing inhibitor PlaB, we realize in vitro culturing of human totipotent stem cells comparable to 2-4-cellb blastomeres Poly(A) RNA-seq is performed on hESCs cultured in MYCP medium

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20795

92 Samples

Download data: CSV

(Submitter supplied) RNA transcripts are distributed non-uniformly in the oocytes of many animals, such that newly-divided embryo cells (blastomeres) inherit distinct transcriptomes following fertilization. In animals such as the frog, Xenopus laevis, programmed transcript regionalization directs early embryonic axis formation and is essential for normal development. However, it is unknown whether such transcriptome asymmetry directs embryogenesis in mammals, or indeed, whether it occurs at all. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL13188

120 Samples

Download data: GPR

(Submitter supplied) We have examined whether twin blastomeres from 2-cell stage mouse embryos differ in mRNA content. Amplified mRNA from 12 blastomeres derived from six embryos approximately mid-way through their second cell cycle was analyzed. Probes displaying normalized values greater than 0.25 were selected and examined for consistent bias in expression within blastomere pairs. Although transcript content varied both between individual embryos and twin blastomeres, no consistent asymmetries were observed for the majority of genes. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL7202

12 Samples

Download data: TXT

(Submitter supplied) Nuclear abnormalities are commonly found in human IVF embryos and are associated with DNA damage, aneuploidy, and decreased developmental potential.

Organism:

Homo sapiens

Type:

SNP genotyping by SNP array; Genome variation profiling by SNP array

Platform:

GPL3718

72 Samples

Download data: CEL, CHP, TXT

(Submitter supplied) Here we modified a single cell whole transcriptome amplification method to make it capable of amplifying cDNAs as long as 3kb efficiently and unbiasedly. We combined this modified single cell cDNA amplification method with Applied Biosystems next generation sequencing SOLiD™ System to set up a single cell whole transcriptome assay. The modified amplification strategy allows us to amplify full-length cDNAs for most of the expressed genes. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL9318

24 Samples

Download data: SRF

(Submitter supplied) HISRainbow mice were used to obtain mosaic ovaries for transcriptome analysis of MII eggs expressing H3.3-eGFP, H3.3R26K-eCFP, or H3.3K27R-mCherry. Results provide comparison of eggs with different genotypes and insight into mechanism of how resting oocytes are selected for ovulation.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

6 Samples

Download data: TXT

(Submitter supplied) The culture conditions of the early preimplantation embryos affects the DNA methylation landscape of the resulting blastocysts.

Organism:

Bos taurus

Type:

Methylation profiling by genome tiling array

Platform:

GPL18384

16 Samples

Download data: TXT

(Submitter supplied) Totipotency is the ability of a single cell to give rise to all the differentiated cells that build the conceptus, yet how to capture this property in vitro remains incompletely understood. Defining totipotency relies upon a variety of assays of variable stringency. Here we describe criteria to define totipotency. We illustrate how distinct criteria of increasing stringency can be used to judge totipotency by evaluating candidate totipotent cell types in the mouse, including early blastomeres and expanded or extended pluripotent stem cells. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL21103 GPL17021

671 Samples

Download data: CSV, LOOM, TXT

(Submitter supplied) Mammalian embryogenesis begins with a totipotent zygote. Early embryogenesis can be recapitulated by aggregated extended pluripotent stem cells (EPSCs) in a 3D culture system. However, the efficiency of generating blastoids is low and whether other reported totipotent stem cells retained a similar capacity remains unknown. Here we show that spliceosomal repressed totipotent blastomere-like cells (TBLCs) form blastocyst-like structures when aggregated in 3D microwells with around 80% efficiency. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL17021 GPL24247

8 Samples

Download data: TXT