Similar studies for GEO DataSets (Select 200107947) - GEO DataSets

Links from GEO DataSets

Items: 20

Select item 2001079471.

Dissection of influenza infection in vivo by single-cell RNA-sequencing

(Submitter supplied) The influenza virus is a major cause of morbidity and mortality worldwide, yet, the impact of intracellular viral invasion and the cellular response diversity remain uncharacterized. By massively parallel single-cell RNA-seq we comprehensively mapped the host lung response to in-vivo influenza infection in wild-type and Irf7-knockout mice across nine immune and non-immune cell types. We found an unexpected high prevalence of infected cells in all cell types, showed that infection is a characteristic property of cell types that is independent of type-I interferon activity, and demonstrated that all cell types responded primarily with a robust generic transcriptional response. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL19057
10 Samples

Download data: TXT

Series

Accession:: GSE107947

ID:: 200107947

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001191232.

Transcriptome analysis of influenza infected GFP+ AEC compared to bystander GFP- AEC

(Submitter supplied) A GFP-expressing recombinant A/Puerto Rico/8/1934 influenza virus was used to infect C57BL/6 wild type mice and on day 3 post infection, lung alveolar epithelial cells (AEC) were isolated and sorted based on GFP expression. GFP+ AEC represent the infected AEC and GFP- AEC represent the bystander AEC. AEC were also sorted from uninfected mice to serve as controls.

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL13112
13 Samples

Download data: TXT

Series

Accession:: GSE119123

ID:: 200119123

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2000615173.

Strand-specific Dual RNA-seq of Bronchial Epithelial cells Infected with Influenza A/H3N2 Viruses Reveals Splicing of Gene Segment 6 and Novel Host-Virus Interactions

(Submitter supplied) Host-influenza virus interplay at the transcript level has been extensively characterized in epithelial cells. Yet, there are no studies that simultaneously characterize human host and influenza A virus (IAV) genomes. We infected human bronchial epithelial BEAS-2B cells with two seasonal IAV/H3N2 strains, Brisbane/10/07 and Perth/16/09 (reference strains for past vaccine seasons) and the well-characterized laboratory strain Udorn/307/72. more...

Organism:: Influenza A virus (A/Perth/16/2009(H3N2)); Homo sapiens; Influenza A virus (A/Udorn/307/1972(H3N2)); Influenza A virus (A/Brisbane/10/2007(H3N2))

Type:: Expression profiling by high throughput sequencing

4 related Platforms
36 Samples

Download data: FA, XLSX

Series

Accession:: GSE61517

ID:: 200061517

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2000660404.

RNA-seq influenza infected B6 & D2 mice

(Submitter supplied) The host response to influenza A infections is strongly influenced by host genetic factors. Animal models of genetically diverse mouse strains are well suitable to identify host genes involved in severe pathology, viral replication and immune responses. Here, we have utilizing a dual RNAseq approach that allowed us to investigate both viral and host gene expression in the same individual from a single expression assay after H1N1 infection. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL16790
36 Samples

Download data: TXT

Series

Accession:: GSE66040

ID:: 200066040

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001159045.

Unique transcriptional architecture in airway epithelial cells and macrophages shapes distinct responses following influenza virus infection ex vivo.

(Submitter supplied) Airway epithelial cells and macrophages differ markedly in their responses to influenza A virus (IAV) infection. To investigate transcriptional responses underlying these differences, purified subsets of type II airway epithelial cells (ATII) and alveolar macrophages (AM) recovered from the lungs of mock- or IAV-infected mice were subjected to RNA sequencing. In the absence of infection, AM predominantly expressed genes related to immunity whereas ATII expressed genes consistent with their physiological roles in the lung. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL17021
96 Samples

Download data: TXT

Series

Accession:: GSE115904

ID:: 200115904

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001478326.

Cell type- and replication stage-specific influenza virus responses in vivo

(Submitter supplied) We used two different influenza viruses lacking either PB1 or HA to differentiate the antiviral responses generated from primary transcription and full replication, respectively.

Organism:: Homo sapiens; Mus musculus

Type:: Expression profiling by high throughput sequencing

Platforms:: GPL17021 GPL24247 GPL24676
184 Samples

Download data: TXT

Series

Accession:: GSE147832

ID:: 200147832

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001127947.

Influenza virus replication intensity and round of infection dictates the cellular response in vivo

(Submitter supplied) Influenza A virus has a broad cellular tropism in the respiratory tract. Infected epithelial cells sense the infection and initiate an antiviral response. To define the antiviral response at the earliest stages of infection we used two different single cycle replication reporter viruses. These tools demonstrated heterogeneity in virus replication levels in vivo. Transcriptional profiling demonstrated tiers of interferon stimulated gene responses that were dependent on the magnitude of virus replication. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL17021
39 Samples

Download data: TXT

Series

Accession:: GSE112794

ID:: 200112794

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001089068.

RNA-seq analysis of miR-324-5p overexpression upon H5N1 infection in A549 cells

(Submitter supplied) The goals of this study are to compare NGS-derived whole transcriptome profiles (RNA-seq) of H5N1 infected A549 cells overexpressing either negative control mimic or miR-324-5p mimic

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL16791
3 Samples

Download data: TXT

Series

Accession:: GSE108906

ID:: 200108906

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 2001146369.

Comprehensive profiling of translation initiation in influenza-virus infected cells

(Submitter supplied) Cellular stress is often accompanied by non-canonical initiation of translation at alternate start codons in mammalian cells. Here we systematically investigate the extent and impact of alternate translation initiation in the context of influenza virus infection. We use ribosome profiling with the initiation inhibitor lactidomycin to experimentally delineate translation initiation sites in a human lung epithelial cell line infected with influenza virus.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing; Other

Platform:: GPL16791
12 Samples

Download data: TSV, TXT

Series

Accession:: GSE114636

ID:: 200114636

PubMed Full text in PMC Similar studies Analyze with GEO2R SRA Run Selector

Select item 20011877310.

Cell-to-cell variation in defective virus expression and effect on host response during influenza virus infection

(Submitter supplied) Virus and host factors contribute to cell-to-cell variation in viral infection and determine the outcome of the overall infection. However, the extent of the variability at the single cell level and how it impacts virus-host interactions at a systems level are not well understood. To characterize the dynamics of viral transcription and host responses, we used single-cell RNA sequencing to quantify at multiple time points the host and viral transcriptomes of human A549 cells and primary bronchial epithelial cells infected with influenza A virus. more...

Organism:: Homo sapiens; Canis lupus familiaris; Influenza A virus (A/Puerto Rico/8/1934(H1N1))

Type:: Expression profiling by high throughput sequencing; Other

7 related Platforms
33 Samples

Download data: TXT

Series

Accession:: GSE118773

ID:: 200118773

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20007050211.

The 1918 PB2 protein, not HA, enhances the virulence of an avian influenza virus closely related to the 1918 pandemic virus through the inhibition of wnt signaling.

(Submitter supplied) The purpose of this experiment was to understand the pathogenic role of individual 1918 genes on the host response to the 1918 pandemic influenza virus. We examined reassortant avian viruses nearly identical to the pandemic 1918 virus (1918-like avian virus) carrying either the 1918 HA or PB2 gene. Both genes enhanced 1918-like avian virus replication, but only the mammalian host adaptation of the 1918-like avian virus through reassortment of the 1918 PB2 led to increased lethality in mice. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL7202
66 Samples

Download data: TXT

Series

Accession:: GSE70502

ID:: 200070502

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20014204712.

Defining effects of in utero arsenic exposure on immune cells in lungs of adult mice exposed to Influenza A with single cell RNA sequencing

(Submitter supplied) Our recent studies have shown that adult mice exposed to inorganic arsenic exclusively in utero (100 ppb via maternal drinking water) exhibit greater lung damage and inflammation following infection with IAV (Influenza A virus, H1N1) than control mice not exposed in utero. We employed an unbiased single cell RNA sequencing approach to conduct a high resolution molecular analysis of recruited immune cells and their transcriptional activities in the lungs of arsenic-exposed and control mice at day 3 after IAV infection. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL19057
6 Samples

Download data: MTX, TSV

Series

Accession:: GSE142047

ID:: 200142047

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20005074213.

Effect of miR-144 and miR-451 expression on lung epithelial cell responses to influenza infection at 1 and 18 hours.

(Submitter supplied) Antiviral responses must be regulated to rapidly defend against infection while minimizing inflammatory damage, but the mechanisms for establishing the magnitude of response within an infected cell are not well understood. miRNAs are small non-coding RNAs that negatively regulate protein levels by binding target sequences on their cognate mRNA. Here we identify miR-144 as a negative regulator of the host antiviral response. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL13912
16 Samples

Download data: TXT

Series

Accession:: GSE50742

ID:: 200050742

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20003195714.

Effect of miR-144/miR451 expression on TC-1 lung epithelial cell responses to influenza infection for 24 hours

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Expression profiling by array; Other

Platforms:: GPL8527 GPL6096
10 Samples

Download data: CEL, TXT

Series

Accession:: GSE31957

ID:: 200031957

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20003195615.

Effect of miR-144/miR451 expression on TC-1 lung epithelial cell responses to influenza infection for 24 hours [RT-PCR]

(Submitter supplied) Antiviral responses must be regulated to rapidly defend against infection while minimizing inflammatory damage, but the mechanisms for establishing the magnitude of response within an infected cell are not well understood. miRNAs are small non-coding RNAs that negatively regulate protein levels by binding target sequences on their cognate mRNA. We profiled microRNA expression in the lungs of mice infected for 24 h with Influenza A/PR/8/34 to identify microRNAs that may regulate host response to influenza infection.

Organism:: Mus musculus

Type:: Other

Platform:: GPL8527
2 Samples

Download data: TXT

Series

Accession:: GSE31956

ID:: 200031956

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20003195516.

Effect of miR-144/miR451 expression on TC-1 lung epithelial cell responses to influenza infection for 24 hours [Expression]

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6096
8 Samples

Download data: CEL

Series

Accession:: GSE31955

ID:: 200031955

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20003152417.

Host cells infected by various types of viruses

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens; Canis lupus familiaris; Gallus gallus; Mus musculus

Type:: Expression profiling by array

4 related Platforms
228 Samples

Download data: CEL, CHP

Series

Accession:: GSE31524

ID:: 200031524

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20003151818.

Host cell gene expression in Influenza A virus (A/Singapore/478/2009 (pH1N1)) infected A549 cells at 2, 4, 6, 8 and 10 hours post infection

(Submitter supplied) We used the microarray data to analyze host cells response on A549 cells infected with Influenza A virus (A/Singapore/478/2009 (pH1N1))