GEO DataSets

(Submitter supplied) Streptococcus agalactiae (Group B Streptococcus, GBS) can colonize the human vaginal tract leading to both superficial and serious infections in adults and neonates. To study bacterial colonization of the reproductive tract in a mammalian system, we employed a murine vaginal carriage model. Using RNASeq, the transcriptome of GBS growing in vivo during vaginal carriage was determined. Over one-quarter of the genes in GBS were found to be differentially regulated during in vivo colonization as compared to laboratory cultures. more...

Organism:

Streptococcus agalactiae A909

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24543

14 Samples

Download data: TXT

(Submitter supplied) Streptococcus pyogenes (Group A Strep, GAS) is a serious human pathogen with the ability to colonize mucosal surfaces such as the nasopharynx and vaginal tract, often leading to infections such as pharyngitis and vulvovaginitis. We present genome-wide RNASeq data showing the transcriptomic changes GAS undergoes during vaginal colonization. These data reveal that the regulon controlled by MtsR, a master metal regulator, is activated during vaginal colonization. more...

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20656

6 Samples

Download data: GTF, TXT

(Submitter supplied) We have conducted RNA-seq analysis on Group B Streptococcus (GBS) when interacting with human stem-cell (hSC) derived BECs. The study contains a control group for GBS and a control group for BECs each with three biological replicates. The last group contains GBS infection BECs and also has three replicates.

Organism:

Homo sapiens; Streptococcus sp. 'group B'

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL31999 GPL18573 GPL31998

9 Samples

Download data: CSV

(Submitter supplied) Transcriptome analysis of Streptococcus agalactiae (group B Streptococcus) grown under control conditions or coincubated with serine hydroxamate to induce the bacterial stringent response

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23396

12 Samples

Download data: TXT

(Submitter supplied) As part of a broader study to identify genes that contribute to fitness of the human pathobiont Streptococcus agalacitae (group B Streptococcus), we identified a GntR-class transcription factor, named mrvR, which contributes to bacterial persistence in human amniotic fluid and multipe virulence phenotypes. In order to understand the transcriptome of mrvR, whole-genome transcriptomic analysis was performed with wild type group B Streptococcus and an mrvR deletion mutant at three growth phases.

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29157

12 Samples

Download data: XLSX

(Submitter supplied) Genes that showed altered expression between wild type A909 and isogenic rgfC/A mutant were identified. A strain with a deletion in RgfC/A (SAK_1917/SAK_1918) was derived and microarray analysis was performed to identify genes regulated by the RgfC/A two component system. strain comparison, global regulation, two component system

Organism:

Streptococcus agalactiae A909; Streptococcus agalactiae

Type:

Expression profiling by array

Platform:

GPL10363

2 Samples

Download data: TXT

(Submitter supplied) Group B Streptococcus (GBS) is a pervasive perinatal pathogen, yet factors driving GBS dissemination in utero are poorly defined. Gestational diabetes mellitus (GDM), a complication marked by dysregulated immunity and maternal microbial dysbiosis, increases risk for GBS perinatal disease. We interrogated host-pathogen dynamics in a novel murine GDM model of GBS colonization and perinatal transmission. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30172

16 Samples

Download data: TXT

(Submitter supplied) Sa strain 874391 and human U937 monocytes at 6h post-infection

Organism:

Streptococcus agalactiae; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL29157 GPL18573 GPL29372

12 Samples

Download data: XLSX

(Submitter supplied) The purpose of this study was to identify Group B Streptococcus (GBS) genes that are controlled by the CiaR response regulator. Deletion of the GBS ciaR gene resulted in a significant decrease in intracellular survival within neutrophils, murine macrophages, and human BMEC, which was linked to increased susceptibility to killing by antimicrobial peptides, lysozyme, and reactive oxygen species. Furthermore, competition experiments in mice showed that wild-type GBS had a significant survival advantage compared to the isogenic ciaR mutant. more...

Organism:

Streptococcus agalactiae

Type:

Expression profiling by array

Platform:

GPL7515

4 Samples

Download data: CEL, CHP, DAT, JPG, RPT

(Submitter supplied) Group B Streptococcus (GBS) is a leading cause of infant sepsis worldwide. Colonization of the gastrointestinal tract is a critical precursor to late-onset disease in exposed newborns. Neonatal susceptibility to GBS intestinal translocation stems from intestinal immaturity; however, the mechanisms by which GBS exploits the immature host remain unclear. β-hemolysin/cytolysin (βH/C) is a highly conserved toxin produced by GBS capable of disrupting epithelial barriers. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

25 Samples

Download data: XLSX

(Submitter supplied) We used RNA-Seq to compare a parental M28 isolate with an isogenic mutant in which the RD2 element had been deleted.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20656

4 Samples

Download data: XLS

(Submitter supplied) In the present investigation, we conducted a bacterial transcriptome dynamics analysis during GBS incubation with human blood. We observed a large modification in the expression of genes involved in transcriptional regulation and in the metabolism of carbohydrates, purins/pyrimidins and inorganic ions, and an up-regulation of genes involved in attachment and plasminogen contact. We also observed differences in the expression of GBS genes in blood at 40° relative to 37°C.

Organism:

Streptococcus agalactiae

Type:

Expression profiling by array

Platform:

GPL6923

34 Samples

Download data: CEL

(Submitter supplied) The purpose of this study was to determine what S. agalactiae genes are under the control of the MtaR regulatory protein. Inactivation of the Streptococcus agalactiae mtaR gene resulted in the downregulation of 11 genes and the upregulation of 1 gene in the mtaR mutant. Genes involved in the uptake of methionine and a gene involved in the degradation of peptides were downregulated. Also, genes involved in the transport and biosynthesis of arginine were downregulated. more...

Organism:

Streptococcus agalactiae

Type:

Expression profiling by array

Platform:

GPL7515

6 Samples

Download data: CEL, CHP

(Submitter supplied) We have discovered that GBS significantly remodels its transcriptome in response to exposure to human amniotic fluid. A large number of the affected genes are of unknown function, which means that much remains to be learned about the full influence of amniotic fluid on GBS. The majority of the observed changes in transcripts affects genes involved in basic bacterial metabolism and is connected to AF composition and nutritional requirements of the bacterium. more...

Organism:

Streptococcus agalactiae

Type:

Expression profiling by array

Platform:

GPL6923

9 Samples

Download data: CEL, CHP

(Submitter supplied) We determined dynamic behavior of S. agalactiae transcriptome during growth in THY medium and detected growth phase regulated genes

Organism:

Streptococcus agalactiae

Type:

Expression profiling by array

Platform:

GPL6923

12 Samples

Download data: CEL

(Submitter supplied) In the present investigation, we conducted a bacterial transcriptome dynamics analysis during GBS incubation with human blood. We observed large modification in the expression of numerous genes, but GBS transcriptome was different with the blood of one donor. More genes were up-regulated and less down-regulated in respect to the other donors.

Organism:

Streptococcus agalactiae

Type:

Expression profiling by array

Platform:

GPL6923

5 Samples

Download data: CEL

(Submitter supplied) To understand the extent to which GBS modify gene expression in response to temperatures encountered in the various hosts, we conducted a whole genome transcriptome analysis of organisms grown at 30°C and 40°C. We identified extensive transcriptome remodeling at various stages of growth, especially in the stationary phase (significant transcript changes occurred for 25% of the genes). A large proportion of genes involved in metabolism was up-regulated at 30°C in stationary phase, which reflects a slowing of bacterial metabolism in the early stages of its shift to growth at lower temperature followed by an acceleration in the later stages. more...

Organism:

Streptococcus agalactiae

Type:

Expression profiling by array

Platform:

GPL6923

18 Samples

Download data: CEL

(Submitter supplied) Diabetic wound infections have poor healing outcomes due to the presence of numerous pathogens in addition to an impaired immune response. Group B Streptococcus (GBS) is one of the most commonly isolated bacteria from diabetic wound infections, but virulence mechanisms GBS uses during these infections have not been investigated. Here, we developed a new murine model of GBS diabetic wound infection to determine how GBS establishes infection and persists in the wound environment. more...

Organism:

Streptococcus agalactiae; Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL32194 GPL32195 GPL13112

15 Samples

Download data: XLSX