GEO DataSets

(Submitter supplied) Blastema formation is a hallmark of limb regeneration that requires proliferation and migration of progenitors derived from many tissues to the amputation plane. To better understand the genetic programs that initiate limb regeneration, we reasoned that blastemal progenitors would be among early proliferating cells in the stump following amputation. Here we separately profiled dividing and non-dividing stump tissues, as well as the wound epidermis, during early axolotl limb regeneration to examine transcriptional programs of blastemal progenitors. more...

Organism:

Ambystoma mexicanum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22800

27 Samples

Download data: CSV, TAB, XLSX

(Submitter supplied) Regeneration of complex multi-tissue structures, such as limbs, requires the coordinated effort of multiple cell types. In axolotl limb regeneration, the wound epidermis and blastema have been extensively studied via histology, grafting, and bulk-tissue RNA-sequencing. However, studying the contributions of these tissues is hindered due to limited information regarding the molecular identity of the cell types in regenerating limbs. more...

Organism:

Ambystoma mexicanum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24679

3 Samples

Download data: FASTA, TXT

(Submitter supplied) Treatment with midkine inhibitor iMDK inhibits limb regeneration. To elucidate the transcriptional mechanisms of iMDK treatment, we sequenced regenerating limbs treated with either DMSO or iMDK (bulk) in biological triplicate at 11 days post-amputation (dpa).

Organism:

Ambystoma mexicanum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24679

6 Samples

Download data: MATRIX

(Submitter supplied) The wound epidermis is required for limb regeneration. To elucidate the roles of the wound epidermis during early regeneration, we examined how the transcriptional programs of early dividing cells (enriched for blastemal progenitors) and non-dividing cells in regenerating stump tissues as well as epithelial cells change when we prevented wound epidermis formation.

Organism:

Ambystoma mexicanum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22800

9 Samples

Download data: MATRIX

(Submitter supplied) Identifying the genetic program that induces limb regeneration in salamanders is an important resource for regenerative medicine, which currently lacks tools to promote regeneration of functional body structures. The genetic network underlying limb regeneration has been elusive due to the complexity of the injury response that occurs concomitant to blastema formation. Here we performed parallel expression profile time courses of non-regenerative lateral wounds versus amputated limbs in axolotl. more...

Organism:

Ambystoma mexicanum

Type:

Expression profiling by array

Platform:

GPL15342

80 Samples

Download data: TXT

(Submitter supplied) Salamander limb regeneration is dependent upon tissue interactions that are local to the amputation site. Communication among limb epidermis, peripheral nerves, and mesenchyme coordinate cell migration, cell proliferation, and tissue patterning to generate a blastema, a mass of progenitor cells that forms missing limb structures. An outstanding question is how molecular cross-talk between these tissues gives rise to the regeneration blastema. more...

Organism:

Ambystoma mexicanum

Type:

Expression profiling by array

Platform:

GPL15153

44 Samples

Download data: CEL

(Submitter supplied) These data provide an important, detailed molecular resource that outlines and defines injury repair and regeneration stage-dependent genes and cellular composition orchestrated by wound healing and blastema stage-associated HDAC activity, which enhances our understanding of this complex limb regeneration process.

Organism:

Ambystoma mexicanum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27159

20 Samples

Download data: TXT

(Submitter supplied) Here we report the results of a robust microarray experiment that examined the first 28 days of axolotl forelimb regeneration. At each of 20 post-amputation time points, we estimated gene expression for 10 replicate RNA samples that were isolated from 1m of heterogeneous tissue collected from the distal limb tip.

Organism:

Ambystoma mexicanum

Type:

Expression profiling by array

Platform:

GPL15153

198 Samples

Download data: CEL, CHP