GEO DataSets

(Submitter supplied) In this study, we analyzed the transcriptomes of ~276k single PBMCs from 33 childhood SLE (cSLE) and 11 healthy matched donors (cHD). Our findings were validated in an independent cohort including 8 adult SLE (aSLE) patients and 6 matched controls (aHD; ~132k PBMCs).

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20301

56 Samples

Download data: MTX, TSV

(Submitter supplied) To investigate ISGs expression chracteristics of leucocyte types of SLE patients, single cell RNA sequencing was applied to 2 SLE patients and 1 health volunteer.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

3 Samples

Download data: MTX, TSV

(Submitter supplied) Gene expression profile studies have identified an interferon signature in whole blood or mononuclear cell samples from patients with systemic lupus erythematosus. This study was designed to determine whether specific lymphocyte and myeloid subsets freshly isolated from the blood of systemic lupus erythematosus patients demonstrated unique gene expression profiles compared to subsets isolated from healthy controls. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4185

Platform:

GPL96

67 Samples

Download data: CEL

Analysis of freshly isolated lymphocyte (CD4+ T cells and CD19+ B cells) and CD33+ myeloid subsets from the blood of Systemic Lupus Erythematosus (SLE) patients. Results provide insight into the molecular mechanisms underlying SLE pathogenesis.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 cell type, 2 disease state sets

Platform:

GPL96

Series:

GSE10325

67 Samples

Download data: CEL

(Submitter supplied) Background/Purpose: Systemic lupus erythematosus (SLE) is a complex multi-system autoimmune disease of uncertain etiology. Patients from different ancestral backgrounds demonstrate differences in clinical manifestations and autoantibody profiles. In this study we examined genome-wide transcriptional patterns in major immune cell subsets across different ancestral backgrounds. Methods: Peripheral blood was collected from 21 African-American (AA) and 21 European-American (EA) SLE patients, 5 AA controls, and 5 EA controls. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

208 Samples

Download data: TXT

(Submitter supplied) Systemic lupus erythematosus (SLE) is a heterogeneous autoimmune disease. Knowledge of circulating immune cell types and cell states associated with SLE remains incomplete. We profiled over 1.2 million PBMCs (162 cases, 99 controls) with multiplexed single-cell RNA-seq (mux-seq). Cases exhibited prominent expression of type-1 interferon-stimulated genes (ISG) in monocytes, reduction of naïve CD4+ T cells that correlated with monocyte ISG expression, and expansion of repertoire-restricted cytotoxic GZMH+ CD8+ T cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

88 Samples

Download data: H5AD

(Submitter supplied) Systemic lupus erythermatosus (SLE) is a complex autoimmune disease, and epigenetic study is promissing for illustrating the mechanisms of SLE pathogenesis. Assay for transposase accessible chromatin in single cells sequencing (scATAC-seq) shows priority to trackle this barrier. Thus, scATAC-seq was applied to difine the landscape of active regulatory DNA in systemic lupus erythermatosus (SLE) at single cell resolusion. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL23227

2 Samples

Download data: BED, MTX, TSV

(Submitter supplied) This study demonstrates quantitative and qualitative differences between type I IFN signatures in autoimmunity and viral infection using purified CD4pos T cells and CD16pos- and CD16neg-monocyte subsets. We were able to discriminate between cell-specific viral response signatures and the pathogenically amplified IFN signatures observed in autoimmunity. The differences were of both a qualitative and quantitative nature, as the signatures in the patients with SLE were characterized by much more complexly compiled gene patterns with increased absolute gene expression levels.

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS4888 GDS4889 GDS4890

Platform:

GPL570

36 Samples

Download data: CEL, CHP

Analysis of CD16+ monocytes from SLE patients and YFV-immunized healthy donors. The YFV immunization can be regarded as a real viral infection, based on clinical/serological manifestations. Results provide insight into differences in type I interferon responses in autoimmunity and viral infection.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state, 7 individual, 3 protocol sets

Platform:

GPL570

Series:

GSE51997

10 Samples

Download data: CEL, CHP

Analysis of CD16- monocytes from SLE patients and YFV-immunized healthy donors. The YFV immunization can be regarded as a real viral infection, based on clinical/serological manifestations. Results provide insight into differences in type I interferon responses in autoimmunity and viral infection.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state, 8 individual, 3 protocol sets

Platform:

GPL570

Series:

GSE51997

12 Samples

Download data: CEL, CHP

Analysis of CD4+ T cells from SLE patients and YFV-immunized healthy donors. The YFV immunization can be regarded as a real viral infection, based on clinical/serological manifestations. Results provide insight into differences in type I interferon responses in autoimmunity and viral infection.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state, 10 individual, 3 protocol sets

Platform:

GPL570

Series:

GSE51997

14 Samples

Download data: CEL, CHP

(Submitter supplied) The highly heterogeneous clinical presentation of lupus is characterized by the unpredictable appearance of flares of disease activity and important organ damage. Attempts to stratify lupus patients have been limited to clinical information, leading to unsuccessful clinical trials and controversial research results. Our aim was to develop and validate a robust method to stratify patients with lupus according to longitudinal disease activity and whole-genome gene expression data in order to establish subgroups of patients who share disease progression mechanisms. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13158

312 Samples

Download data: CEL

(Submitter supplied) Whole blood expression was profiled in Rheumatoid Arthiritis and SLE (Systemic LUPUS Erythomatosus) patients.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13158

805 Samples

Download data: CEL

(Submitter supplied) Lupus, a server and complex autoimmune disease, is clinically divided into cutaneous lupus erythematosus (CLE) which featured in skin damage, and systemic lupus erythematosus (SLE) which characterized in systemic multi-organ damage. The distinction of these two types of lupus is widely unknown. Here, we collected 23 skin biopsies of healthy control(HC), DLE (discoid lupus erythematosus, a main type of CLE) and SLE, separated epidermis and dermis and performed single cell RNA sequencing through microfluidics based 10x genomics system. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

30 Samples

Download data: MTX, TAR, TSV

(Submitter supplied) Epienome-wide DNA methylation profiling of systemic lupus erythematosus (SLE). The Illumina HumanMethylation450K Beadchip was used to obtain DNA methylation profiles across approximately 450,000 CpGs in normal human blood samples from females. Samples included 33 non-SLE female patients (control) and 57 SLE female patients. SLE patients:- Ethnicity included 39 African americans and 18 European Americans. more...

Organism:

Homo sapiens

Type:

Methylation profiling by array

Platform:

GPL13534

90 Samples

Download data: IDAT

(Submitter supplied) Systemic lupus erythematosus patients exhibit remarkable heterogeneity in clinical manifestations and autoantibody repertoires. This complexity poses major barrier in diagnosis and effective treatment of SLE. To address this we studied the SLE patients in groups categorized on the basis of distinct sera autoantibodies. SLE patients were segregated into three group based on the presence of autoantibodies against i) dsDNA only ii) ENA (extractable nuclear antigens) only or iii) both.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

16 Samples

Download data: XLSX

(Submitter supplied) To examine mononuclear cell gene expression profiles in patients with and without SLE and subsets with and without atherosclerosis

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

72 Samples

Download data: TXT

(Submitter supplied) Objective: Systemic lupus erythematosus (SLE) has limited monozygotic (MZ) twin concordance, implying a role for other pathogenic factors than genetic variation, such as epigenetic changes. Using the disease discordant twin model, we investigated genome-wide DNA methylation changes in sorted CD4+ T-cells, monocytes, granulocytes and B-cells in twin pairs with at least one SLE-affected twin. Methods: Peripheral blood from 15 SLE twin pairs (six MZ, nine dizygotic (DZ)) was processed using gradient density centrifugation for the granulocyte fraction. more...

Organism:

Homo sapiens

Type:

Methylation profiling by genome tiling array

Platform:

GPL13534

104 Samples

Download data: CSV, IDAT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Methylation profiling by genome tiling array

Platforms:

GPL13534 GPL10558

110 Samples

Download data

(Submitter supplied) Our study has demonstrated that significant number of differential genes in SLE was involved in IFN, TLR signaling pathways and inflammatory cytokines. The enrichment of differential genes has been associated with aberrant DNA methylation, which may be relevant to the pathogenesis of SLE. Our observations laid the groundwork for further diagnostic and mechanistic studies of SLE and LN.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

55 Samples

Download data: TXT