GEO DataSets

(Submitter supplied) Here, we show the scRNA-seq-based analysis of 9 ILC samples sorted by flow cytometry, from blood, healthy livers, hepatocellular carcinoma (HCC) tumors, and HCC tumor-adjacent liver tissues. Unbiased transcriptional clustering revealed seven distinct ILC subpopulations in healthy liver and six in liver cancer, among which there were two tissue-resident NK cell subpopulations (CXCR6+CD16+ and CXCR6+CD16-) in healthy liver but only one tissue-resident NK cell subpopulation (CXCR6+CD16-) in tumor tissues. Notably, ILC2s were significantly accumulated in the HCC tissues, and bulk RNA sequencing of SLAMF1+ ILC2s and SLAMF1- ILC2s identified their function.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

13 Samples

Download data: MTX, TSV, TXT

(Submitter supplied) Natural killer (NK) cells are innate cytotoxic and immunoregulatory lymphocytes that have a central role in anti-tumor immunity and play a critical role in mediating cellular immunity in advanced cancer immunotherapies, such as dendritic cell (DC) vaccines. Our group recently tested a novel recombinant adenovirus-transduced autologous DC-based vaccine that simultaneously induces T cell responses against three melanoma-associated antigens for advanced melanoma patients. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL25998

41 Samples

Download data: RCC, TXT

(Submitter supplied) Natural killer cells are cytotoxic innate lymphoid cells that play an important role for early host defenses against infectious pathogens and surveillance against tumor growth and metastasis. In humans, NK cells may be divided in various subsets on the basis of the relative expression of the CD56 molecule and of the low-affinity FcγRIIIA CD16. In particular, the two main NK cell subsets are represented by the CD56bright CD16-/dull and the CD56dull CD16bright NK cells. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL18402

20 Samples

Download data: TXT

(Submitter supplied) Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer-related death world-wide. The prevalence of non-alcoholic fatty liver disease (NAFLD) has been rising, along with an increase in NAFLD-related HCC. To investigate cell-type composition in NAFLD and HCC, we performed single-nucleus RNA-seq (snRNA-seq) of patients with fatty liver disease in order to transcriptionally characterize cell-types in an unbiased manner. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30982

6 Samples

Download data: MTX, TSV

(Submitter supplied) In this study we performed single-cell RNA sequencing on 7000 negatively enriched fresh NK and progenitor cells from one healthy bone marrow donor, that were pre-labeled with oligonucleotide-conjugated antibodies against CD34, CD117, CD56 and CXCR6. A new CD56dimGzmK+ subset was identified that shared features with CD56bright and CD56dimGzmK- NK cells based on transcriptome, phenotype (NKG2AhighCD16lowKLRG1highTIGIThigh) and functional analysis in bone marrow and blood, supportive for an intermediate subset. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

1 Sample

Download data: H5, MTX, TSV

(Submitter supplied) Purified NK cells from human liver tissues were first enriched by MACS using NK Cell Isolation Kit (Miltenyi Biotec, German) and CD49a+/- hepatic NK cells were isolated by FACS Aria cell sorter (BD Biosciences, United States) to attain a purity greater than 95%.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

2 Samples

Download data: TXT

(Submitter supplied) Pooled purified peripheral blood derived CD56dimCD16+ NK, CD56brightCD16- NK and in vitro activated CD56+CD16+ NK subsets obtained from 9 healthy donors were analyzed for gene expression pattern. Each pooled NK subset sample was hybridized in replicates (A and B). Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS751

Platform:

GPL1313

6 Samples

Download data

Expression profiling of CD56dimCD16+, CD56brightCD16-, and in vitro activated CD56+CD16+ natural killer (NK) cells. NK cells derived from purified peripheral blood pooled from 9 healthy donors.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 cell type, 2 protocol sets

Platform:

GPL1313

Series:

GSE1511

6 Samples

Download data

(Submitter supplied) Liver-resident NK (lrNK) cells have been studied in human as well as in mice. In the current study, we compared the transcriptome of three porcine NK cell populations: CD8αhigh cNK cells from peripheral blood, CD8αhigh NK cells isolated from the liver and the liver-specific CD8αdim NK cells using RNA-seq, to gain new insights in the biology of these cells. Overall, our data indicate that lrNK cells show an immature and anti-inflammatory phenotype. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20983

15 Samples

Download data: CSV

(Submitter supplied) The liver plays a critical role in both immune defense and tolerance in the body. The liver-resident immune cells (LrICs) determine the immune properties, but the unique composition and heterogeneity of these cells are incompletely understood. Here, we dissect the diversity of LrICs by a comprehensive transcriptomic profiling using the unbiased single-cell RNA-sequencing (scRNA-seq). A total of 70, 706 of CD45+ immune cells from the paired liver perfusion, spleen and peripheral blood as references were profiled. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20795

9 Samples

Download data: MTX, TSV