GEO DataSets

(Submitter supplied) In our study, we found that MetR as a transcriptional regulator may regulate the expression of genes not involved in methionine synthesis. To verify our hypothesis, we firstly construct a strain with metR over-expression in D39 and with 3×flag tag and culture this strain in chemically defined medium (CDM) without methionine. Then collect bacteria for ChIP-seq to identify the genes that can be directly transcriptionally regulated by MetR.

Organism:

Streptococcus pneumoniae D39

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL29748

4 Samples

Download data: XLSX

(Submitter supplied) metR (SPD_0588) mutant (TH9197) cannot grow in chemically defined medium (CDM) and had significant growth defect in CDM with 1 µg/ml methionine. With the increasing concentration of mehtionine, the growth of TH9197 was restored and the growth of metR mutant is as same as D39 WT (TH4306) when the concentration of methionine reaches 50 µg/ml. SPD_0588 is a putative transcriptional regulator and is homologous to MetR in Streptococcus mutans (78.9 % amino acid identity), which regulates both methionine synthesis and transport. more...

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24758 GPL22606

8 Samples

Download data: XLSX

(Submitter supplied) Streptococcus pneumoniae normally resides in the human nasopharynx in a non-disease state. In response to yet unknown triggers it can descend to the lower respiratory tract and/or invade the bloodstream. Regulation and activation of virulence genes play essential roles in this process of disease development. A putative transcriptional regulator in S. pneumoniae, MgrA, with homology to a virulence gene activator, mga, of Group A streptococcus (GAS) was previously identified as being required for development of pneumonia in a murine model. more...

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by array

Platform:

GPL2712

16 Samples

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(Submitter supplied) We were interested in determining if the LuxS signaling system contributed to the regulation of pneumococcal genes. To accomplish this, we compared the in vitro transcriptional profiles over time of S. pneumoniae D39, a serotype 2 strain of pneumococcus, with that of an isogenic deletion mutant, (delta)luxS, using a spotted S. pneumoniae- specific DNA microarray. For in vitro time course experiments, broth cultures of D39 and (delta)luxS were grown to low density (optical densities between ~0.01- 0.02), the cells were collected by centrifugation, washed 1x with sterile PBS, back diluted to ~3x105CFU/ ml into fresh broth, and immediately incubated at 37oC in a 5% CO2 chamber for 45 minutes. more...

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by array

Platform:

GPL2712

40 Samples

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(Submitter supplied) We characterize the translational and transcriptional programs induced by MetR and investigate the potential mechanisms through which methionine regulates gene expression, using the budding yeast S. cerevisiae as the model organism. Using ribosomal profiling and RNA-seq, we systematically compared the translational and transcriptional profiles of cells growing in the normal and methionine restricted media. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13821

4 Samples

Download data: WIG

(Submitter supplied) In order to elucidate the regulatory role of pneumococcal TCS08, a transcriptomic approach was performed using microarray from bacterial cultures in chemical defined medium of single and double tcs08 mutants. A total of 159 genes presenting significant expression changes were selected for further evaluation and discussion. These 159 genes were classified accordingly to their biochemical function.

Organism:

Streptococcus pneumoniae TIGR4; Streptococcus pneumoniae

Type:

Expression profiling by array

Platform:

GPL24482

12 Samples

Download data: TXT, XLSX

(Submitter supplied) Streptococcus pneumoniae is an opportunistic human pathogen that typically colonizes the nasopharyngeal passage and causes lethal disease in other host niches such as the lung or the meninges. How pneumococcal genes are expressed and regulated at the different stages of its life cycle, as commensal or as pathogen, has not been entirely described. To chart the transcriptional responses of S. pneumoniae, we quantified the transcriptome under 22 different infection-relevant conditions. more...

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24384

34 Samples

Download data: TXT