Similar studies for GEO DataSets (Select 200168728) - GEO DataSets

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Items: 20

Select item 2001687281.

Mouse totipotent stem cells captured and maintained through spliceosomal repression

(Submitter supplied) Since the establishment of the first embryonic stem cells (ESCs), in vitro culture of totipotent cells functionally and molecularly comparable to in vivo blastomeres with embryonic and extraembryonic developmental potency is unviable. Spliceosomes are responsible for mRNA splicing and maturation. Here, we report that spliceosomal repression in mouse ESCs drives pluripotent-to-totipotent state transition. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Methylation profiling by high throughput sequencing

Platforms:: GPL24247 GPL21273 GPL17021
50 Samples

Download data: COV, CSV, NARROWPEAK

Series

Accession:: GSE168728

ID:: 200168728

PubMed Similar studies SRA Run Selector

Select item 2002262312.

Capturing totipotency in human cells through spliceosomal repression

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Expression profiling by array; Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL20795
112 Samples

Download data: BW, NARROWPEAK, TXT

Series

Accession:: GSE226231

ID:: 200226231

PubMed Similar studies

Select item 2002262293.

Capturing totipotency in human cells through spliceosomal repression [ATAC-seq]

(Submitter supplied) Supplemented with splicing inhibitor PlaB, we realize in vitro culturing of human totipotent stem cells comparable to 2-4-cell blastomeres Assay for transposase accessible chromatin with high-throughput sequencing in hESC sand hTBLCs

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL20795
2 Samples

Download data: NARROWPEAK

Series

Accession:: GSE226229

ID:: 200226229

PubMed Similar studies

Select item 2002262284.

Capturing totipotency in human cells through spliceosomal repression [scRNA-seq]

(Submitter supplied) Supplemented with splicing inhibitor PlaB, we realize in vitro culturing of human totipotent stem cells comparable to 2-4-cell blastomeres

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL20795
14 Samples

Download data: TXT

Series

Accession:: GSE226228

ID:: 200226228

PubMed Similar studies

Select item 2002262275.

Capturing totipotency in human cells through spliceosomal repression [ChIP-seq]

(Submitter supplied) Supplemented with splicing inhibitor PlaB, we realize in vitro culturing of human totipotent stem cells comparable to 2-4-cell blastomeres Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for h3k4me3 and h3k27me3 in hESC sand hTBLCs

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL20795
4 Samples

Download data: BW

Series

Accession:: GSE226227

ID:: 200226227

PubMed Similar studies

Select item 2002247946.

Capturing totipotency in human cells through spliceosomal repression [RNA-Seq]

(Submitter supplied) Supplemented with splicing inhibitor PlaB, we realize in vitro culturing of human totipotent stem cells comparable to 2-4-cellb blastomeres Poly(A) RNA-seq is performed on hESCs cultured in MYCP medium

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL20795
92 Samples

Download data: CSV

Series

Accession:: GSE224794

ID:: 200224794

Select item 2000451827.

RNA-seq analysis of ES cell populations with different functional potentials in serum/LIF and 2i/LIF, as well as analysis of the transcriptional effect of LIF in ground state (2i) culture conditions.

(Submitter supplied) We report that ES cells cultured in ground state (2i and 2i/LIF) culture conditions are heterogeneous and show heterogeneus expression of extraembryonic markers. Using a highly sensitive reporter for the endoderm marker Hex we can sort Hex high and low populations from either serum/LIF or 2i/LIF and demonstrate that they have different functional properties. Here we explored the transcriptional basis of these functional differences and noted that Hex low (HV-) and Hex high (HV+) populations showed more distinct expression profiles in 2i/LIF than in serum/LIF. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL14602
12 Samples

Download data: TXT

Series

Accession:: GSE45182

ID:: 200045182

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001456098.

Defining totipotency using criteria of increasing stringency

(Submitter supplied) Totipotency is the ability of a single cell to give rise to all the differentiated cells that build the conceptus, yet how to capture this property in vitro remains incompletely understood. Defining totipotency relies upon a variety of assays of variable stringency. Here we describe criteria to define totipotency. We illustrate how distinct criteria of increasing stringency can be used to judge totipotency by evaluating candidate totipotent cell types in the mouse, including early blastomeres and expanded or extended pluripotent stem cells. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platforms:: GPL21103 GPL17021
671 Samples

Download data: CSV, LOOM, TXT

Series

Accession:: GSE145609

ID:: 200145609

PubMed Similar studies SRA Run Selector

Select item 2001850059.

Induction of mouse totipotent stem cells by a defined chemical cocktail

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing; Methylation profiling by high throughput sequencing

Platform:: GPL24247
18 Samples

Download data: BW, MTX, NARROWPEAK, TSV

Series

Accession:: GSE185005

ID:: 200185005

PubMed Similar studies

Select item 20018500410.

Induction of mouse totipotent stem cells by a defined chemical cocktail [RRBS]

(Submitter supplied) We demonstrate induction and long-term maintenance of totipotent stem cells (TotiSCs) from mouse pluripotent stem cells (PSCs) by a combination of three small molecules, TTNPB, 1-Azakenpaullone, and WS6. These cells, which we designated as ciTotiSCs (chemically induced totipotent stem cells), resembled mouse totipotent 2C-embryo stage cells at both transcriptome and epigenome level.

Organism:: Mus musculus

Type:: Methylation profiling by high throughput sequencing

Platform:: GPL24247
2 Samples

Download data: BW

Series

Accession:: GSE185004

ID:: 200185004

PubMed Similar studies SRA Run Selector

Select item 20018500111.

Induction of mouse totipotent stem cells by a defined chemical cocktail [scRNA-seq]

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL24247
2 Samples

Download data: MTX, TSV

Series

Accession:: GSE185001

ID:: 200185001

PubMed Similar studies SRA Run Selector

Select item 20018500012.

Induction of mouse totipotent stem cells by a defined chemical cocktail [bulk RNA-seq]

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL24247
10 Samples

Download data: CSV

Series

Accession:: GSE185000

ID:: 200185000

PubMed Similar studies SRA Run Selector

Select item 20018499913.

Induction of mouse totipotent stem cells by a defined chemical cocktail [ATAC-seq]

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL24247
4 Samples

Download data: BW, NARROWPEAK

Series

Accession:: GSE184999

ID:: 200184999

PubMed Similar studies SRA Run Selector

Select item 20019777914.

Highly efficient generation of blastocyst-like structures from mouse totipotent blastomere-like cells

(Submitter supplied) Mammalian embryogenesis begins with a totipotent zygote. Early embryogenesis can be recapitulated by aggregated extended pluripotent stem cells (EPSCs) in a 3D culture system. However, the efficiency of generating blastoids is low and whether other reported totipotent stem cells retained a similar capacity remains unknown. Here we show that spliceosomal repressed totipotent blastomere-like cells (TBLCs) form blastocyst-like structures when aggregated in 3D microwells with around 80% efficiency. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platforms:: GPL17021 GPL24247
8 Samples

Download data: TXT

Series

Accession:: GSE197779

ID:: 200197779

PubMed Similar studies

Select item 20003689615.

RNA-Seq from wt and G9A knockout ES cells

(Submitter supplied) To measure gene expression difference between wt and g9A knockout ES cells

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL13112
2 Samples

Download data: BED

Series

Accession:: GSE36896

ID:: 200036896

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20003392316.

2C::tomato ES cells, 2-cell embryos and wild type oocytes

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platforms:: GPL13112 GPL6246 GPL9250
16 Samples

Download data: BED, CEL

Series

Accession:: GSE33923

ID:: 200033923

PubMed Full text in PMC Similar studies

Select item 20003392217.

RNA-Seq from wt oocytes

(Submitter supplied) To determine gene expression in mouse ovulated oocytes

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL9250
3 Samples

Download data: BED

Series

Accession:: GSE33922

ID:: 200033922

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20003392118.

RNA-Seq from two-cell (2C) stage embryos

(Submitter supplied) To determine gene expression in 2 cell stage embryos

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL13112
3 Samples

Download data: BED

Series

Accession:: GSE33921

ID:: 200033921

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20003392019.

mRNA-Seq of 2C::tomato+ vs. - ES cells

(Submitter supplied) We identified/quantified genes and repeat elements enriched within 2C::tomato+ cells vs. 2C::tomato - cells

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL9250
2 Samples

Download data: BED

Series

Accession:: GSE33920

ID:: 200033920

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20003376320.

Expression data from 2C::tomato+ vs 2C::tomato - ES cells

(Submitter supplied) We compared gene expression from 2C::tomato+/- ES cells from Kdm1a wt and mutant ES cultures 2C::tomato- samples 1, 5, 9 2C::tomato+ samples 2, 6, 10