GEO DataSets

(Submitter supplied) Adiponectin-mediated pathways contribute to mammalian homeostasis; however, little is known about adiponectin and adiponectin receptor signaling in arthropods. In this study, we demonstrate that Ixodes scapularis ticks have an adiponectin receptor-like protein (ISARL) but lack adiponectin, suggesting activation by alternative pathways. ISARL expression is significantly upregulated in the tick gut after Borrelia burgdorferi infection, suggesting that ISARL signaling may be co-opted by the Lyme disease agent. more...

Organism:

Ixodes scapularis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29893

18 Samples

Download data: TXT

(Submitter supplied) To understand the immunomodulation roles of complement C1q-like protein 3 (IsC1ql3), we utilized RNA sequencing (RNA-seq) to investigate the effect of IsC1ql3 on the murine transcriptome.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

6 Samples

Download data: TXT

(Submitter supplied) Tick gut and salivary gland global gene expression change after feeding on adiponectin wildtype and knockout mice

Organism:

Ixodes scapularis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29893

4 Samples

Download data: TXT

(Submitter supplied) An Ixodes scapularis saliva mRNA vaccine induces tick resistance and prevents Borrelia burgdorferi infection in guinea pigs

Organism:

Cavia porcellus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22346

14 Samples

Download data: TXT

(Submitter supplied) Splenocytes comprised a wide-range of immune cells responsible for the protection against infectious diseases. We exposed splenocytes to GFP+ Borrelia, sorted GFP+ and GFP- splenocytes and performed single-cell RNA seq to analyze the DEGs between infected and bystander population in different immune cell populations

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

4 Samples

Download data: MTX, TSV

(Submitter supplied) We performed comparative transcriptomic analysis of the outer membrane vesicles (OMVs) released from B. burgdorferi. We identified a total of ~1200 unique transcripts with at least one mapped read from the bacterial cell and its OMVs.

Organism:

Borreliella burgdorferi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24990

3 Samples

Download data: TXT

(Submitter supplied) We report the results of gene expression profiling via RNA-sequencing of primary human choroid plexus epithelial cells in vitro that were infected with Borrelia burgdorferi, the causative agent of Lyme disease. Epithelial cells were cultured with B. burgdorferi for 48 hours prior to RNA isolation. Following high-throughput sequencing via Illumina HiSeq 4000, a total of 258 differentially expressed genes were identified. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL20301

6 Samples

Download data: TXT

(Submitter supplied) Lyme disease (LD), caused by Borrelia burgdorferi, is the most common tick-borne infectious disease in the United States. We examined gene expression patterns in the blood of individuals with early disseminated LD at the time of diagnosis (Acute LD) and also at approximately 1 month and 6 months following antibiotic treatment. A distinct acute LD profile was observed that was sustained during early convalescence (1 month) but returned to control levels six months after treatment. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13667

86 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24676 GPL16791

12 Samples

Download data: MTX, TSV

(Submitter supplied) We performed single-nucleus RNA sequencing (snRNA-seq) of skin and blood of persons presenting with naturally acquired, attached Ixodes scapularis ticks.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

3 Samples

Download data: MTX, TSV

(Submitter supplied) We performed RNA sequencing of skin and blood of persons presenting with naturally acquired, attached Ixodes scapularis ticks.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

9 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL15520 GPL11154

15 Samples

Download data: TXT

(Submitter supplied) mRNA profiles of astrocytes infected with Borrelia burdorferi for 24 hours, 48 hours, and 24 hour uninfected controls were generated by deep sequencing, in triplicate, using Illumina HiSeq.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

9 Samples

Download data: TXT

(Submitter supplied) miRNA profiles of astrocytes infected with Borrelia burgdorferi for 24 hours, 48 hours, and 24 hour uninfected controls were generated by deep sequencing, in duplicate, using Illumina MiSeq.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15520

6 Samples

Download data: CSV

(Submitter supplied) Dendritic cells bridge the innate and adaptive immune response by serving as sensors of infection and as the primary antigen presenting cells responsible for the initiation of the T cell response against invading pathogens. Initial interactions between B. burgdorferi, the causative agent of Human Lyme disease, and dendritic cells remain largely unexplored. To address this, we cultured monocyte-derived dendritic cells (moDCs) with and without the Toll-Like Receptor 2 agonist LTA or live B. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

91 Samples

Download data: TXT

(Submitter supplied) Borreliella burgdorferi is the causative agent of Lyme disease, which is the most common vector-borne disease in the United States. While cases of Lyme disease are geographically limited to the Northeast, mid-Atlantic, and Midwest states, there are still estimates of almost 500,000 cases annually in the United States alone. In this study, we sought to assess transcriptional changes that occur in response to B. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

10 Samples

Download data: H5