GEO DataSets

(Submitter supplied) Fluoxetine (FLX) is the most common selective serotonin reuptake inhibitor pharmaceutical used for the treatment of psychological disorders such as depression and anxiety. Increased prescription use of FLX by human populations has led to its pseudo-persistence in effluent-receiving waterways. Despite emerging concerns of FLX becoming a potential threat to the aquatic environment, there is currently limited information on effects of FLX on aquatic wildlife, including its specific mechanism of action and associated toxicity pathways in fish. more...

Organism:

Pimephales promelas

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30333

30 Samples

Download data: CSV

(Submitter supplied) Current methods to support chemical hazard assessment face significant challenges given the rapidly growing number of compounds of emerging concern (CEC) requiring assessment. Standard testing strategies rely upon live animal testing, which are time-consuming, expensive, and ethically questionable. These concerns serve as an impetus to develop new approach methodologies (NAMs) that do not rely on live animal tests. more...

Organism:

Pimephales promelas

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30333

26 Samples

Download data: CSV, TXT

(Submitter supplied) The goal of this study is to characterize molecular toxicity pathways associated with ethinylestradiol-induced toxicity and link these molecular perturbations to apical outcomes of regulatory relevance.

Organism:

Pimephales promelas

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25572

15 Samples

Download data: CSV, TXT

(Submitter supplied) Triclocarban (TCC) is a widely used antimicrobial agent that is routinely detected in surface waters. The present study was designed to examine TCC’s efficacy and mode of action as a reproductive toxicant in fish. Reproductively mature Pimephales promelas were continuously exposed to either 1 or 5 μg TCC/L, 0.5 μg 17β-trenbolone (TRB)/L or a mixture (MIX) of 5 μg TCC and 0.5 μg TRB/L for 22 d and a variety of reproductive and endocrine-related endpoints were examined. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL10259

24 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL17098

60 Samples

Download data: TXT

(Submitter supplied) Abstract — Within the US military, new insensitive munitions (IMs) are rapidly replacing conventional munitions improving safety from unintended detonation. Toxicity data for IM chemicals are expanding rapidly, however IM constituents are typically deployed in mixture formulations, and very little is known about their mixture toxicology. In the present study we sought to characterize the mixture effects and toxicology of the two predominant IM formulations IMX-101 and IMX-104 in acute (48 h) larval fathead minnow (Pimephales promelas) exposures. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL17098

12 Samples

Download data: TXT

(Submitter supplied) Abstract — Within the US military, new insensitive munitions (IMs) are rapidly replacing conventional munitions improving safety from unintended detonation. Toxicity data for IM chemicals are expanding rapidly, however IM constituents are typically deployed in mixture formulations, and very little is known about their mixture toxicology. In the present study we sought to characterize the mixture effects and toxicology of the two predominant IM formulations IMX-101 and IMX-104 in acute (48 h) larval fathead minnow (Pimephales promelas) exposures. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL17098

12 Samples

Download data: TXT

(Submitter supplied) Abstract — Within the US military, new insensitive munitions (IMs) are rapidly replacing conventional munitions improving safety from unintended detonation. Toxicity data for IM chemicals are expanding rapidly, however IM constituents are typically deployed in mixture formulations, and very little is known about their mixture toxicology. In the present study we sought to characterize the mixture effects and toxicology of the two predominant IM formulations IMX-101 and IMX-104 in acute (48 h) larval fathead minnow (Pimephales promelas) exposures. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL17098

12 Samples

Download data: TXT

(Submitter supplied) Abstract — Within the US military, new insensitive munitions (IMs) are rapidly replacing conventional munitions improving safety from unintended detonation. Toxicity data for IM chemicals are expanding rapidly, however IM constituents are typically deployed in mixture formulations, and very little is known about their mixture toxicology. In the present study we sought to characterize the mixture effects and toxicology of the two predominant IM formulations IMX-101 and IMX-104 in acute (48 h) larval fathead minnow (Pimephales promelas) exposures. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL17098

12 Samples

Download data: TXT

(Submitter supplied) Abstract — Within the US military, new insensitive munitions (IMs) are rapidly replacing conventional munitions improving safety from unintended detonation. Toxicity data for IM chemicals are expanding rapidly, however IM constituents are typically deployed in mixture formulations, and very little is known about their mixture toxicology. In the present study we sought to characterize the mixture effects and toxicology of the two predominant IM formulations IMX-101 and IMX-104 in acute (48 h) larval fathead minnow (Pimephales promelas) exposures. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL17098

12 Samples

Download data: TXT

(Submitter supplied) We evaluated the possible mechanisms by which exposure to a sequentially treated pulp and paper mill effluent affects gene expression in the liver of male and female fathead minnows.

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL7282

24 Samples

Download data: TXT

(Submitter supplied) Prochloraz is a fungicide known to cause endocrine disruption through effects on the hypothalamic-pituitary-gonadal (HPG) axis. To determine the short-term impacts of prochloraz on gene expression and steroid production, adult female fathead minnows (Pimephales promelas) were exposed to the chemical (0 or 300 μg/L) for a time-course of 6, 12 and 24 h. Consistent with inhibition of cytochrome P450 17α-hydroxylase/17,20-lyase (CYP17) and aromatase (CYP19), known molecular targets of prochloraz, plasma 17β-estradiol (E2) was reduced within 6 hours. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL9248

23 Samples

Download data: TXT

(Submitter supplied) Abstract — Previous toxicological investigations of the insensitive munition (IM), 3-nitro-1,2,4-triazol-5-one (NTO), demonstrated histopathological and physiological impacts in mammalian testes. The implications of these findings for fish was unknown, therefore we investigated the effects of chronic (21 day) exposures to NTO and an NTO-containing IM formulation called IMX-101 (composed of 2,4-dinitroanisole (DNAN), nitroguanidine (NQ), and NTO) in adult male fathead minnows to assess if impacts on testes were conserved. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL17098

10 Samples

Download data: TXT

(Submitter supplied) Abstract — Previous toxicological investigations of the insensitive munition (IM), 3-nitro-1,2,4-triazol-5-one (NTO), demonstrated histopathological and physiological impacts in mammalian testes. The implications of these findings for fish was unknown, therefore we investigated the effects of chronic (21 day) exposures to NTO and an NTO-containing IM formulation called IMX-101 (composed of 2,4-dinitroanisole (DNAN), nitroguanidine (NQ), and NTO) in adult male fathead minnows to assess if impacts on testes were conserved. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL17098

20 Samples

Download data: TXT

(Submitter supplied) Despite major successes in reducing the risks of lead (Pb) exposure over the past few decades, two issues of considerable importance remain unresolved: (1) how differences in water chemistry influence acute and chronic Pb toxicity, and (2) the elucidation of specific toxic mechanisms and modes of action (MOA). To more clearly define the water chemistry parameters mediating Pb toxicity we evaluated the effects of hardness (as CaSO4) and DOC (as humic acid (HA)) during chronic (150d) exposures to the fathead minnow (Pimephales promelas). more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL5443

30 Samples

Download data: GPR

(Submitter supplied) Abstract: The Army is replacing traditional munitions with insensitive munitions (IM) resistant to accidental detonation. Although the parent IM compound nitroguanidine (NQ) is generally not acutely toxic at concentrations >1000 mg/L in aquatic exposures, products formed by intensive UV-degradation resulted in of multiple-order of magnitude increase in toxicity. A methylated congener of NQ, 1-methyl-3-nitroguanidine (MeNQ), is also being assessed for potential use in IM explosive formulations and consequently was here investigated for UV-degradation hazard and bioaccumulation potential. more...

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL17098

40 Samples

Download data: TXT

(Submitter supplied) Gene expression profiles of fathead minnow brain tissue following treatment to fluoxetine (Prozac), carbamazepine, venlafaxine, and a mixture of all three; compared to untreated controls.

Organism:

Pimephales promelas

Type:

Expression profiling by array

Platform:

GPL7351

16 Samples

Download data: TXT

(Submitter supplied) RNA-seq expression assays were done in larval Pimephales promelas (Fathead minnow, FHM) exposed to 10 chemicals (3 metals, 4 Neonicotinoids, and 3 pharmaceuticals) over a range of concentrations to derive relatively short term transcriptomics based points of departure (tPODs). tPODs were compared to apical points of departures (aPODs) to investigate the potential use of the FHM tPODS as part of a high throughput approach that might be used in a regulatory context.

Organism:

Pimephales promelas

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25572

878 Samples

Download data: TXT