GEO DataSets

(Submitter supplied) Cuscuta campestris is an obligate stem parasite which uses an organ called the haustoria to divert water and photosynthates from the host.  Previously, we have identified that at the haustorial interface between Cuscuta campestris and Arabidopsis thaliana, miRNAs generated by the parasite are able to move into the host and regulate host gene expression.  This study identifies how long after attachment does trans-species miRNA transcription begin in Arabidopsis thaliana and Solanum lycopersicum, as well as identifying which stage of haustoria development they become detectable.  A time course was performed by harvesting interfaces every 24 hours post attachment, and samples were subjected to RNA extraction and sRNA sequencing. more...

Organism:

Solanum lycopersicum

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL29032

42 Samples

Download data: CSV

(Submitter supplied) Cuscuta campestris is an obligate parasitic plant that attaches to the stems of host plants to obtain water and nutrients. C. campestris produces a specialized set of microRNAs specifically at the host-parasite interface. Many of these interface-induced microRNAs target mRNAs from the host. This experiment was designed to capture full-length primary transcripts that give rise to C. campestris interface-induced microRNAs. more...

Organism:

Cuscuta campestris

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32961

9 Samples

Download data: TSV

(Submitter supplied) The parasitic plant Cuscuta campestris produces specialized microRNAs that are specifically expressed at the haustorial interface. Some of these "Interface-Induced MicroRNAs" function to target host mRNAs. C. campestris haustoria can be induced in the absence of any host tissues using a combination of light and physical pressure. This experiment tested with such in vitro, host-free haustoria produced interface-induced microRNAs. more...

Organism:

Cuscuta campestris

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL32307

9 Samples

Download data: CSV

(Submitter supplied) Cuscuta campestris is an obligate stem parasite which uses an organ called the haustoria to divert water and photosynthates from the host.  Previously, we have identified that at the haustorial interface between Cuscuta campestris and Arabidopsis thaliana, miRNAs generated by the parasite are able to move into the host and regulate host gene expression.  This study identifies how long after attachment does trans-species miRNA transcription begin in Arabidopsis thaliana and Solanum lycopersicum, as well as identifying which stage of haustoria development they become detectable.  A time course was performed by harvesting interfaces every 24 hours post attachment, and samples were subjected to RNA extraction and sRNA sequencing. more...

Organism:

Arabidopsis thaliana

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL24270

50 Samples

Download data: CSV

(Submitter supplied) Dodders (Cuscuta spp.) are obligate parasitic plants that obtain water and nutrients from the stems of host plants via specialized feeding structures called haustoria. Dodder haustoria facilitate bi-directional movement of viruses, proteins, and mRNAs between host and parasite, but the functional effects of these movements are not clear. Here we show that C. campestris haustoria accumulate high levels of many novel microRNAs (miRNAs) while parasitizing Arabidopsis thaliana hosts. more...

Organism:

Cuscuta pentagona; Arabidopsis thaliana

Type:

Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL22255 GPL22256 GPL17639

6 Samples

Download data: TXT

(Submitter supplied) High-throughput sequencing of small RNAs derived from different tissues of Orobanche plants.

Organism:

Phelipanche aegyptiaca

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL30484

2 Samples

Download data: FASTA, TXT

(Submitter supplied) Parasitism is a successful life strategy that has evolved independently in several families of Higher Plants. In parasitic plants, nutrients and water are obtained from their hosts through specifically adapted haustoria. The genera Cuscuta and Orobanche represent examples for the two profoundly different groups of parasites, one parasitizing host shoots and the other infecting host roots. In this study, we sequenced and described the overall repertoire of small RNAs (sRNome) from Cuscuta campestris and compared it to an exploratory sRNome from Orobanche aegyptiaca. more...

Organism:

Cuscuta campestris

Type:

Other; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL30483

13 Samples

Download data: FASTA, TXT

(Submitter supplied) Cuscuta campestris is an obligate parasitic plant that requires a host to complete its lifecycle. Parasite-host connections occur via an haustorium, a unique organ that acts as a bridge for the uptake of water, nutrients and macromolecules. Research on Cuscuta is often complicated by host influences, but comparable systems for growing the parasite in the absence of a host do not exist. We developed an axenic method to grow C. more...

Organism:

Cuscuta campestris

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30290

8 Samples

Download data: TXT

(Submitter supplied) An intriguing new paradigm in plant biology is that systemically-mobile mRNAs play a role in coordinating development. In this process, specific mRNAs are loaded into the phloem transport stream for translocation to distant tissues, where they may impact developmental processes. However, despite its potential significance for plant growth regulation, mRNA trafficking remains poorly understood and challenging to study. more...

Organism:

Solanum lycopersicum; Cuscuta pentagona

Type:

Expression profiling by array

Platform:

GPL4741

10 Samples

Download data: CEL