GEO DataSets

(Submitter supplied) To characterize the differential effects of LIF in BAE vs BCE cells, genes induced/suppressed by LIF were analyzed by RNA-seq following incubation with LIF. Remarkably, LIF treatment led to distinct gene expression patterns in these two cell types.

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23295

8 Samples

Download data: TXT

(Submitter supplied) In this study, we further verified the role of LIF by looking at gene expressions in morula stage embryos through DNA microarray. Our results showed that LIF knockdown affected 373 gene expressions, and after LIF supplementation we found that the epidermal growth factor (EGF) is most affected by LIF expression. In order to observe the correlation between LIF and EGF, the LIF knockdown embryos were supplemented with various growth factors including LIF, EGF, GM-CSF, TGF, and IGF II. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL872

2 Samples

Download data: TXT

(Submitter supplied) Cachexia is an exacerbating event in many types of cancer that is strongly associated with a poor prognosis. We have identified cytokine, signaling and transcription factors that are required for cachexia in the mouse C26 colon carcinoma model of cancer. C2C12 myotubes treated with conditioned medium from C26 cancer cells induced atrophy and activated a STAT-dependent reporter gene but not reporter genes dependent on SMAD, FOXO, C/EBP, NF-ĸB, or AP-1. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL19485

12 Samples

Download data: CEL, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Expression profiling by array

Platforms:

GPL11202 GPL17021

8 Samples

Download data: TXT

(Submitter supplied) T helper 17 (Th17) cell differentiation triggered by interleukin-6 (IL-6) via STAT3 activation promotes inflammation in inflammatory bowel disease (IBD) patients. However, leukemia inhibitory factor (LIF), an IL-6 family cytokine, restricts inflammation by blocking Th17 cell differentiation via an unknown mechanism. Here, we report that microbiota dysregulation promotes LIF secretion by intestinal epithelial cells (IECs) in a mouse colitis model. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

4 Samples

Download data: TXT

(Submitter supplied) We report that microbiota dysregulation promotes LIF secretion by intestinal epithelial cells (IECs) in a mouse colitis model. LIF greatly activates STAT4 phosphorylation on multiple SPXX elements within the C-terminal transcription regulation domain. STAT4 and STAT3 act reciprocally on both canonical cis-inducible elements (SIEs) and noncanonical “AGG” elements at different loci. In lamina propria lymphocytes (LPLs), STAT4 activation by LIF blocks STAT3-dependent Il-17a/Il-17f promoter activation, whereas in IECs, LIF bypasses the extraordinarily low level of STAT4 to induce YAP gene expression via STAT3 activation. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

4 Samples

Download data: XLSX

(Submitter supplied) Purpose: The goals of this study were to identify cell specific expression patterns of the retinal pigment epithelium (RPE) and a variety of choroidal cell types originating from the macula and the periphery of human donor eyes, with a particular emphasis on identifying expression signatures of choroidal endothelial cells. Methods: Independent libraries were prepared for macular and peripheral samples of combined RPE/choroid from seven donors in two single cell sequencing experiments. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20301

14 Samples

Download data: TSV

(Submitter supplied) Leukemia inhibitory factor (LIF) is amongst the IL-6 family cytokines expressed in the lungs during pneumonia. However, the function of endogenous LIF during pneumonia has never been explored. The purpose of this study was to determine the transcriptional response to pneumonia in the lungs and whether or how this response is influenced by LIF. Mice received intratracheal instillations of vehicle (PBS) or Escherichia coli (10^6 CFU). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS4583

Platform:

GPL11078

9 Samples

Download data: CEL

Analysis of lung from C57BL/6 animals during E. coli pneumonia in the presence of neutralizing anti-leukemia inhibitory factor (LIF) antibody. Anti-LIF exacerbates lung injury. Results provide insight into molecular mechanisms underlying the pathophysiology of pneumonia.

Organism:

Mus musculus

Type:

Expression profiling by array, transformed count, 2 infection, 2 protocol sets

Platform:

GPL11078

Series:

GSE34901

9 Samples

Download data: CEL