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Links from GEO DataSets

Items: 19

1.
Full record GDS2324

Low concentrations of 17beta-estradiol effect on breast cancer cell line

Analysis of breast cancer MCF7/BUS cells treated with 17beta-estradiol (E2) at concentrations up to 100 pM. Results compared to that from an experiment examining the response to hormone starvation (GDS2323). Results provide insight into the E2 sensitivity of E2 inducible or suppressible genes.
Organism:
Homo sapiens
Type:
Expression profiling by array, count, 5 dose sets
Platform:
GPL96
Series:
GSE4668
25 Samples
Download data
2.

Expression data from hormone-starved MCF7/BUS cell culture

(Submitter supplied) To obtain comprehensive information on 17beta-estradiol (E2) sensitivity of genes that are inducible or suppressible by this hormone, we designed a method that determines ligand sensitivities of large numbers of genes using DNA microarray and a set of simple Perl computer scripts implementing the standard metric statistics, and employed it to characterize effects of low (0-100 pM) concentrations of E2 on the transcriptome profile of MCF7/BUS human breast cancer cells, whose E2 dose-dependent growth curve saturated with 100 pM E2. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Datasets:
GDS2323 GDS2324
Platform:
GPL96
34 Samples
Download data
Series
Accession:
GSE4668
ID:
200004668
3.
Full record GDS2323

Estrogen-starved breast cancer cell line: time course

Analysis of breast cancer MCF7/BUS cells starved of estrogen for up to 2 days. Results compared to that from an experiment (GDS2324) examining the response to low concentrations of 17beta-estradiol (E2). Results provide insight into the E2 sensitivity of E2 inducible or suppressible genes.
Organism:
Homo sapiens
Type:
Expression profiling by array, count, 3 time sets
Platform:
GPL96
Series:
GSE4668
9 Samples
Download data
4.

Genomic analyses of breast cancer cells: 17β-hydroxysteroid dehydrogenase type 1 induces transcriptional changes in estradiol-dependent and independent manners

(Submitter supplied) 17β-HSD1 expression modulates T47D transcript profile in in steroid-deprived medium. The enzyme specifically regulates apoptosis and cancer-related genes in T47D cells cultured in steroid-deprived medium. Genes that primarily involved in Cell cycle progression, such as the Cyclin A2 gene, CCNA2, are generally down-regulated whereas most genes involved in apoptosis and cell death, such as the proapoptotic gene XAF1 and FGF12, are on the contrary up-regulated by 17β-HSD1 knockdown, and 21% of the modulated genes belong to this latter functional category. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL6244
8 Samples
Download data: CEL
Series
Accession:
GSE77345
ID:
200077345
5.

Effects of estrogen

(Submitter supplied) Effects of estrogen on global gene expression: identification of novel targets of estrogen action. Keywords: other
Organism:
Homo sapiens
Type:
Expression profiling by SAGE
Platform:
GPL4
4 Samples
Download data
Series
Accession:
GSE41
ID:
200000041
6.

Contribution of HSD17B12 for estradiol biosynthesis in human breast cancer

(Submitter supplied) 17beta-hydroxysteroid dehydrogenase type12 (HSD17B12) has been demonstrated to be involved in regulation of in situ biosynthesis of estradiol (E2). HSD17B12 expression was reported in breast carcinomas but its functions have remained unknown. Therefore, we examined the correlation between mRNA expression profiles determined by microarray analysis and tissue E2 concentrations obtained from 16 postmenopausal breast carcinoma cases in order to analyze an association of the enzyme expression with intratumoral E2 production. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Platforms:
GPL96 GPL97
32 Samples
Download data: CEL, CHP
Series
Accession:
GSE11965
ID:
200011965
7.

Profiling of three different ER+ breast cancer cell lines grown in the presence and absence of estrogen.

(Submitter supplied) Three human ER+ breast cancer cell lines--MCF-7, T47-D, BT-474--grown with or without estradiol (E2). Keywords: Cell Line Comparison
Organism:
Homo sapiens
Type:
Expression profiling by array
Dataset:
GDS1549
Platform:
GPL96
12 Samples
Download data: CEL
Series
Accession:
GSE3529
ID:
200003529
8.
Full record GDS1549

Estrogen effect on estrogen receptor alpha positive breast cancer cell lines

Expression profiling of estrogen receptor positive breast cancer cell lines treated with estradiol for 24 hours. MCF-7, T47-D, and BT-474 breast cancer cell lines examined. Results identify candidate genes involved in estrogen stimulated breast cancer growth.
Organism:
Homo sapiens
Type:
Expression profiling by array, transformed count, 2 agent, 3 cell line sets
Platform:
GPL96
Series:
GSE3529
12 Samples
Download data: CEL
DataSet
Accession:
GDS1549
ID:
1549
9.

Novel Estrogen Receptor-{alpha} Binding Sites and Estradiol Target Genes Identified by ChIP Cloning in Breast Cancer.

(Submitter supplied) Estrogen receptor-{alpha} (ER{alpha}) and its ligand estradiol play critical roles in breast cancer growth and are important therapeutic targets for this disease. Using chromatin immunoprecipitation (ChIP)-on-chip, ligand-bound ER{alpha} was recently found to function as a master transcriptional regulator via binding to many cis-acting sites genome-wide. Here, we used an alternative technology (ChIP cloning) and identified 94 ER{alpha} target loci in breast cancer cells. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Dataset:
GDS3283
Platform:
GPL570
9 Samples
Download data: CEL
Series
Accession:
GSE11506
ID:
200011506
10.
Full record GDS3283

Estradiol effect on breast cancer cell line: time course

Analysis of MCF-7 breast cancer cells treated with estradiol for 3 or 6 hours. Results combined with ChIP experiments to identify estrogen receptor alpha binding sites and estradiol target genes in breast cancer cells.
Organism:
Homo sapiens
Type:
Expression profiling by array, transformed count, 2 agent, 2 time sets
Platform:
GPL570
Series:
GSE11506
9 Samples
Download data: CEL
DataSet
Accession:
GDS3283
ID:
3283
11.

Estrogen effects on MCF-7 breast cancer cells co-expressing ERa and ERb

(Submitter supplied) Two subtypes of the estrogen receptor, ERalpha and ERbeta, mediate the actions of estrogens, and the majority of human breast tumors contain both ERalpha and ERbeta. To examine the possible interactions and modulatory effects of ERbeta on ERalpha activity, we have used adenoviral gene delivery to produce human breast cancer (MCF-7) cells expressing ERbeta, along with their endogenous ERalpha. We have examined the effects of ERβ expression on genome-wide gene expression by Affymetrix GeneChip microarrays. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Dataset:
GDS2770
Platform:
GPL96
12 Samples
Download data
Series
Accession:
GSE4006
ID:
200004006
12.
Full record GDS2770

Estrogen effect on breast cancer cell line coexpressing estrogen receptors alpha and beta

Analysis of estrogen receptor (ER) alpha positive MCF-7 breast cancer cells following introduction of ERbeta and treatment with estrogen. The majority of breast cancers express both ERalpha and ERbeta. Results provide insight into the comodulatory effects of these two ERs.
Organism:
Homo sapiens
Type:
Expression profiling by array, count, 2 agent, 3 infection sets
Platform:
GPL96
Series:
GSE4006
12 Samples
Download data
DataSet
Accession:
GDS2770
ID:
2770
13.

Estrogen Coordinates Translation and Transcription Revealing a Role for NRSF in Human Breast Cancer Cells

(Submitter supplied) Analysis of estrogen receptor (ER)-positive MCF7 cell total RNA expression and polysome-assiciated RNA expression following treatment with estradiol (E2) and vehicle (etoh). We used expression microarrays to measure polysome association and total RNA abundance in E2 treated MCF7. These data, along with previously published data, show that genes that are upregulated by estrogen treatment are biased towards association with polysomes.
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL6244
18 Samples
Download data: CEL
Series
Accession:
GSE18592
ID:
200018592
14.

Gene Regulation by Estrogen Signaling and DNA Methylation in MCF7 Breast Cancer Cells

(Submitter supplied) Estrogen signaling and epigenetic modifications, in particular DNA methylation, are involved in regulation of gene expression in breast cancers. Here we investigated a potential regulatory cross-talk between these two pathways by identifying their common target genes and exploring potential underlying molecular mechanisms in human MCF7 breast cancer cells. Principal Findings: Gene expression profiling revealed that the expression of approximately 150 genes was influenced by both 17β-estradiol (E2) and a hypomethylating agent 5-aza-2’-deoxycytidine (DAC). more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL13158
12 Samples
Download data: CEL, XLS
Series
Accession:
GSE36683
ID:
200036683
15.

The expression patterns of 17b-estradiol responsive genes in wt MCF7, OHT resistant MCF7 and ICI resistant MCF7

(Submitter supplied) Compare the expression pattern of 17b-estradiol responsive genes in parent, OHT-resistant and ICI-resistant breast cancer cells. Keywords: 17b-estradiol responsive genes, OHT resistance, Fulvestrand resistance
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL570
24 Samples
Download data
Series
Accession:
GSE5840
ID:
200005840
16.

The genomic response of a human uterine endometrial adenocarcinoma cell line to 17alpha-ethynyl estradiol.

(Submitter supplied) We have determined the gene expression profile induced by 17 alpha-ethynyl estradiol (EE) in Ishikawa cells, a human uterine-derived estrogen-sensitive cell line, at various doses (1 pM, 100 pM, 10 nM, and 1 microM) and time points (8, 24, and 48 h). The transcript profiles were compared between treatment groups and controls (vehicle-treated) using high-density oligonucleotide arrays to determine the expression level of approximately 38,500 human genes. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL570
75 Samples
Download data: CEL, CHP
Series
Accession:
GSE11869
ID:
200011869
17.

Expression data from MCF7/BUS cell culture exposed to xenoestrogens

(Submitter supplied) MCF7 cells were exposed to xenoestrogens (vehicle = 0.1% ethanol, 30 pM 17beta-estradiol, 3 micromolar genistein, 10 micromolar genistein, 1 micomolar daidzein, 10 nM bisphenol A, 10 nM para-nonylphenol, 3 nM PPT) for 48 hours. Keywords: xenoestrogen response
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL96
24 Samples
Download data
Series
Accession:
GSE5200
ID:
200005200
18.

A genomic view of estrogen actions in human breast cancer cells

(Submitter supplied) Changes in the expression of 8400 genes were monitored by cDNA microarray analysis during the first 32 h of human breast cancer (BC) ZR-75.1 cell stimulation with a mitogenic dose of 17beta-estradiol, a timing which corresponds to completion of a full mitotic cycle in hormone-stimulated cells. Hierarchical clustering of 344 genes whose expression either increases or decreases significantly in response to estrogen reveals that the gene expression program activated by the hormone in these cells shows 8 main patterns of gene activation/inhibition. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL970
25 Samples
Download data
Series
Accession:
GSE1864
ID:
200001864
19.

Xenoestrogen Dose-dependent Transcriptomal Changes in MCF-7 Human Breast Cancer Cells

(Submitter supplied) Transcriptome analysis of MCF-7 cells exposed for 48 hours to various concentrations of xenoestrogen chemicals. Although biological effects of endocrine disrupting chemicals (EDCs) are often observed at unexpectedly low doses with occasional non-monotonic dose-response characteristics, transcriptome-wide profiles of sensitivities or dose-dependent behaviors of the EDC responsive genes have remained unexplored. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL570
351 Samples
Download data: CEL, CHP
Series
Accession:
GSE50705
ID:
200050705
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