GEO DataSets

Analysis of adult differentiating CD34+ hematopoietic progenitor cells at various time points up to 11 days of growth in serum-free medium containing erythropoietin, interleukin-3 and stem cell factor. Results provide insight into the molecular basis of erythropoiesis.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 6 time sets

Platform:

GPL570

Series:

GSE4655

18 Samples

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(Submitter supplied) computational analysis of transcriptomic information during erythroid development Keywords: erythroid differentiation

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2431

Platform:

GPL570

18 Samples

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(Submitter supplied) K562 cells (duplicate cultures A & B) are treated with 50 micromolar hemin for 0, 6, 12, 24, 48, 72 hours followed by RNA extraction and gene expression profiling on Affymetrix human U133A arrays and analysis by MAS 5.0 Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS826

Platform:

GPL96

12 Samples

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Expression profiling of K562 erythroleukemia cells induced to differentiate into erythroid-like cells by hemin. K562 cells examined at various time points up to 72 hours following treatment with 50 uM hemin. Results provide insight into mechanisms underlying hemoglobinization.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 6 time sets

Platform:

GPL96

Series:

GSE1036

12 Samples

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(Submitter supplied) d-serine is naturally present throughout the human body. It is also used as add-on therapy for treatment-refractory schizophrenia. d-Serine interacts with the strychnine-insensitive glycine binding site of NMDA receptor, and this interaction could lead to potentially toxic activity (i.e., excitotoxicity) in brain tissue. The transcriptomic changes that occur in the brain after d-serine exposure have not been fully explored. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS3643

Platform:

GPL1355

24 Samples

Download data: CEL

Analysis of forebrains of animals treated with up to 500 mg/kg D-serine for 96 hours. D-serine is involved in many physiological processes through its interaction with the glycine binding site of the NMDA receptor. Results provide insight into the impact of D-serine exposure on neuronal functions.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent, 6 dose sets

Platform:

GPL1355

Series:

GSE10748

24 Samples

Download data: CEL

(Submitter supplied) Objective: Thyroid hormone receptors (TRs) are ligand-dependent transcription factors with a major impact on erythroid cell development. Here we investigated TR activity on red cell gene expression and identified TR target genes. The impact of the TR target gene GAR22 (growth arrest specific 2 [GAS2]-related gene on chromosome 22) on red cell differentiation was determined. Methods: SCF/Epo dependent red cell progenitors were differentiated in vitro in the presence or absence of thyroid hormone. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL8300

13 Samples

Download data: CEL, CHP, EXP

(Submitter supplied) Peripheral blood stem and progenitor cells (PBSC) are widely used for autologous and allogeneic hematopoietic stem cell transplantation. PBSC can be mobilized into the peripheral blood using cytokines, cytotoxic chemotherapy or a combination of both. Granulocyte colony stimulating factor (G-CSF) has become the most commonly administered cytokine for PBSC mobilization because of its high potency and lack of serious toxicity. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2321

Platform:

GPL201

16 Samples

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Analysis of granulocyte colony stimulating factor (G-CSF) or polyethylene glycol-conjugated (pegylated) G-CSF mobilized CD34+ hematopoietic stem and progenitor cells from multiple myeloma patients undergoing chemotherapy. Pegylated G-CSF has a longer half-life than unconjugated G-CSF.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent sets

Platform:

GPL201

Series:

GSE4688

16 Samples

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(Submitter supplied) CD34+ progenitors were isolated from the bone marrow of three healthy volunteers. CD34+CD71+CD45RA- were FACS sorted to enrich for erythroid progenitors. The cells were cultured for four hours with or without EPO in combination with LY294002, and harvested for RNA extraction, amplification and expression analysis. A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2448

Platform:

GPL3528

12 Samples

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Analysis of CD34+ erythroid progenitors stimulated with erythropoietin (Epo) with or without LY294002, a PI3K inhibitor. Epo promotes differentiation and proliferation of early progenitors via activation of PI3K. Results provide insight into molecular mechanisms underlying the effects of Epo.

Organism:

Homo sapiens

Type:

Expression profiling by array, log2 ratio, 4 growth protocol sets

Platform:

GPL3528

Series:

GSE6260

12 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

6 Samples

Download data: CEL

(Submitter supplied) In order to investigate the role of CD34 antigen in haematopoietic commitment, we overexpressed the human CD34 cDNA in human CD34+ cells by retroviral gene transfer. Keywords: treatment comparison

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

3 Samples

Download data: CEL

(Submitter supplied) In order to investigate the role of CD34 antigen in haematopoietic commitment, we silenced the CD34 gene expression in CD34+ stem/progenitor cells using a siRNA approach. Keywords: treatment comparison

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

3 Samples

Download data: CEL

(Submitter supplied) In this study we have identified the target genes of SREBP1a and SREBP1c in primary cultures of human skeletal muscle cells using adenoviral vectors expressing the mature nuclear form of human SREBP1a or SREBP1c combined with oligonucleotide microarrays. Keywords: comparison of human myotubes infected either by SREBP1a or 1C

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1456

6 Samples

Download data: GPR

(Submitter supplied) We investigated the miRNA expression in ex vivo human erythroid cultures from K562 cells. Hypothetically, the decline of certain miRNAs may promote erythropoiesis by unblocking expression of key functional proteins while the up-regulation of other miRNAs may block commitment to non-erythroid lineages. By comparison with the miRNA expression changes in human umbilical cord blood-derived CD34 cells, signature miRNAs for erythroid development can be discovered. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL4400

8 Samples

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(Submitter supplied) We investigated the miRNA expression in ex vivo human erythroid cultures from umbilical cord blood (UCB)-derived CD34 cells. Hypothetically, the decline of certain miRNAs may promote erythropoiesis by unblocking expression of key functional proteins while the up-regulation of other miRNAs may block commitment to non-erythroid lineages. Therefore, discovering the patterns and sequence of miRNA expression during hematopoietic differentiation will provide insights about the functional roles of these tiny noncoding genes. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL4400

6 Samples

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(Submitter supplied) The complete platform showing three replicates per miRNA on the chip Protocol: Probeset was obtained from Ambion. Construction of the probeset, printing of probset on to array, hybridization and detection protocols can be obtained from Ambion.

Organism:

Homo sapiens

2 Series

14 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by array

Platforms:

GPL1261 GPL570

22 Samples

Download data: CEL

(Submitter supplied) Differences in the amount of fetal hemoglobin (HbF) that persists into adulthood affect the severity of sickle cell disease and the beta-thalassemia syndromes. Genetic association studies have identified sequence variants in the gene BCL11A that influence HbF levels. Here we examine BCL11A as a potential regulator of HbF expression. The high HbF BCL11A genotype is associated with reduced BCL11A expression. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

12 Samples

Download data: CEL