GEO DataSets

Analysis of CD34+ erythroid progenitors stimulated with erythropoietin (Epo) with or without LY294002, a PI3K inhibitor. Epo promotes differentiation and proliferation of early progenitors via activation of PI3K. Results provide insight into molecular mechanisms underlying the effects of Epo.

Organism:

Homo sapiens

Type:

Expression profiling by array, log2 ratio, 4 growth protocol sets

Platform:

GPL3528

Series:

GSE6260

12 Samples

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(Submitter supplied) CD34+ progenitors were isolated from the bone marrow of three healthy volunteers. CD34+CD71+CD45RA- were FACS sorted to enrich for erythroid progenitors. The cells were cultured for four hours with or without EPO in combination with LY294002, and harvested for RNA extraction, amplification and expression analysis. A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2448

Platform:

GPL3528

12 Samples

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(Submitter supplied) Objective: Thyroid hormone receptors (TRs) are ligand-dependent transcription factors with a major impact on erythroid cell development. Here we investigated TR activity on red cell gene expression and identified TR target genes. The impact of the TR target gene GAR22 (growth arrest specific 2 [GAS2]-related gene on chromosome 22) on red cell differentiation was determined. Methods: SCF/Epo dependent red cell progenitors were differentiated in vitro in the presence or absence of thyroid hormone. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL8300

13 Samples

Download data: CEL, CHP, EXP

(Submitter supplied) We investigated the miRNA expression in ex vivo human erythroid cultures from K562 cells. Hypothetically, the decline of certain miRNAs may promote erythropoiesis by unblocking expression of key functional proteins while the up-regulation of other miRNAs may block commitment to non-erythroid lineages. By comparison with the miRNA expression changes in human umbilical cord blood-derived CD34 cells, signature miRNAs for erythroid development can be discovered. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL4400

8 Samples

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(Submitter supplied) We investigated the miRNA expression in ex vivo human erythroid cultures from umbilical cord blood (UCB)-derived CD34 cells. Hypothetically, the decline of certain miRNAs may promote erythropoiesis by unblocking expression of key functional proteins while the up-regulation of other miRNAs may block commitment to non-erythroid lineages. Therefore, discovering the patterns and sequence of miRNA expression during hematopoietic differentiation will provide insights about the functional roles of these tiny noncoding genes. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL4400

6 Samples

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(Submitter supplied) The complete platform showing three replicates per miRNA on the chip Protocol: Probeset was obtained from Ambion. Construction of the probeset, printing of probset on to array, hybridization and detection protocols can be obtained from Ambion.

Organism:

Homo sapiens

2 Series

14 Samples

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(Submitter supplied) computational analysis of transcriptomic information during erythroid development Keywords: erythroid differentiation

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2431

Platform:

GPL570

18 Samples

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Analysis of adult differentiating CD34+ hematopoietic progenitor cells at various time points up to 11 days of growth in serum-free medium containing erythropoietin, interleukin-3 and stem cell factor. Results provide insight into the molecular basis of erythropoiesis.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 6 time sets

Platform:

GPL570

Series:

GSE4655

18 Samples

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(Submitter supplied) In response to elevated glucocorticoid levels, erythroid progenitors rapidly expand to produce large numbers of young erythrocytes. Previous work demonstrates hematopoietic changes in rodents exposed to various physical and psychological stressors, however, the effects of chronic psychological stress on erythropoiesis has not be delineated. We employed laboratory, clinical and genomic analyses of a murine model of chronic restraint stress (RST) to examine the influence of psychological stress on erythropoiesis. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

8 Samples

Download data: CEL

(Submitter supplied) Understanding the pattern of gene expression and identifying the specific genes expressed during erythropoiesis is crucial for a synthesis of erythroid developmental biology. Here we have isolated four distinct populations of erythroblasts at successive erythropoietin-dependent stages of erythropoiesis including the terminal, pyknotic stage. The transcriptome has been determined using Affymetrix arrays. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3860

Platform:

GPL570

16 Samples

Download data: CEL

Analysis of 4 discrete populations of erythroid progenitors at successive erythropoietin-dependent stages of erythropoiesis: CFU-E, Pro-E, Int-E and Late-E. Unsorted erythroid cells at each stage also examined. Results provide insight into the molecular mechanisms underlying erythroblast maturation.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 4 development stage, 2 protocol sets

Platform:

GPL570

Series:

GSE22552

16 Samples

Download data: CEL

(Submitter supplied) The formation of red blood cells begins with the differentiation of multipotent haematopoietic progenitors. Reconstructing the steps of this differentiation represents a general challenge in stem-cell biology. Here we used single-cell transcriptomics, fate assays and a theory that allows the prediction of cell fates from population snapshots, to demonstrate that mouse hematopoietic progenitors differentiate through a continuous, hierarchical structure into seven blood lineages. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL19057 GPL13112

9 Samples

Download data: CSV, TXT

(Submitter supplied) The fetal hemoglobin (HbF) levels were 95.4 ± 1.5% and 4.4 ± 0.2% in fetal liver-derived and adult blood derived-cultured erythrocytes (n=5), respectively. Following RNA isolation from the CD71 high/GPA positive erythroblasts, gene expression analyses were performed using Affymetrix Human Gene 2.0 ST Array. The Affymetrix raw data files of microarray were preprocessed using robust multi-array average method for background correction, log-transformation, and quantile normalization. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16686

10 Samples

Download data: CEL

(Submitter supplied) Activation of the AKT and ERK signaling pathway is a major contributor to cell proliferation. However, the integrated regulation of this multistep process, involving signal processing, cell growth and cell-cycle progression, is poorly understood. Here we study three cell types of hematopoietic origin, in which AKT and ERK signaling is triggered by erythropoietin (Epo). We find that the different cell types exhibit distinct proliferative responses, despite sharing the molecular network for pro-proliferative signaling. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

16 Samples

Download data: CEL

(Submitter supplied) We used scRNAseq to profile CD71/CD24low fetal liver erythroid progenitor cells isolated by 2 distinct methods: FACS and immunomagnetic isolation. Cells from both isolation methods were hashtagged using Biolegend mouse hashtag antibodies and library prepped together on the 10X chromium platform with the 3'RNA v3 kit. We also performed CITE-seq to profile proteogenomic expression of CD117 and CD71 on lineage-depleted mouse fetal liver erythroid progenitor cells. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL19057 GPL17021

2 Samples

Download data: CSV

(Submitter supplied) Single cell RNA sequencing of freshly isolated mouse burst forming unit-erythroid (BFU-E).

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

24 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

940 Samples

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(Submitter supplied) Single cell mouse BFU-E (burst forming unit-erythroid ) were FACS-deposited into individual wells of a 96-well plate containing PCM either with or without 100 nM dexamethasone. After 16hrs cells from wells that contained a single pair of daughter cells were separated and each individual daughter cell transcriptome was obtained by single cell RNA-seq.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

52 Samples

Download data: TXT

(Submitter supplied) single cell RNA sequencing of freshly isolated mouse BFU-E (burst forming unit-erythroid ) cells cultured for 1, 2, or 3 days with and without 100nM dexamethasone

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

576 Samples

Download data: TXT

(Submitter supplied) Single cell RNA sequencing of freshly isolated mouse burst forming unit-erythroid (BFU-E) , colony forming unit-erythroid (CFU-E), and intermediate stages of erythroid development cells.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

96 Samples

Download data: TXT