GEO DataSets

Analysis of corpus lutea (CLs) of animals treated with prostaglandin F2alpha (PGF2alpha). The CL is a transient endocrine gland that produces progesterone, a hormone needed to maintain pregnancy. Results provide insight into the mechanisms underlying the regression of CL mediated by PGF2alpha.

Organism:

Macaca mulatta; Macaca radiata

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL3535

Series:

GSE7971

6 Samples

Download data: CEL, CHP, EXP

(Submitter supplied) Corpus luteum (CL) is an ephemeral gland whose main function is to secrete progesterone required for the establishment and maintenance of pregnancy. In non-fertile cycles, primate CL has a finite life span of 14-16 days, following which it undergoes regression. Although it has been suggested long time back that PGF2alpha of intra-ovarian or intra-luteal origin acts as a physiological luteolysin in primates, the mechanisms by which PGF2alpha mediates its luteolytic actions are poorly understood. more...

Organism:

Macaca radiata; Macaca mulatta

Type:

Expression profiling by array

Dataset:

GDS3039

Platform:

GPL3535

6 Samples

Download data: CEL, CHP, EXP

(Submitter supplied) Prostaglandin F2 alpha (PGF2alpha) brings about regression of the bovine corpus luteum (CL). This luteolytic property of PGF2alpha is used in the beef and dairy cattle to synchronize estrus. A limitation of this protocol is an insensitivity of the early CL to luteolytic actions of PGF2alpha. The mechanisms underlying luteal sensitivity (LS) are poorly understood. The early CL has maximum number of PGF2alpha receptors; therefore differences in signaling events might be responsible for LS. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL3810

1 Sample

Download data: GPR

(Submitter supplied) A large body of evidence suggests that the development and maintenance of corpus luteum (CL) in primates requires the action of LH. Earlier, using CET-induced luteolysis model, we demonstrated changes in luteal transcriptome suggesting nuclear actions of LH in the primate CL. To further demonstrate the role of LH in maintenance of primate CL, replacement studies were carried out and it was observed that administration of a single injection of rhLH was sufficient to restore the progesterone to pre-CET treatment levels and prevent the CET-induced luteolysis. more...

Organism:

Macaca radiata; Macaca mulatta

Type:

Expression profiling by array

Platform:

GPL3535

4 Samples

Download data: CEL, CHP

(Submitter supplied) Corpus luteum (CL) is an ephemeral gland whose main function is to secrete progesterone required for the establishment and maintenance of pregnancy. It is very well established that development and maintenance of CL function in primates requires action of luteinizing hormone (LH) but the extent and mechanism by which LH contributes to the maintenance of CL function through out the luteal phase is not known. more...

Organism:

Macaca mulatta; Macaca radiata

Type:

Expression profiling by array

Platform:

GPL3535

6 Samples

Download data: CEL, CHP

(Submitter supplied) Next-generation sequencing (RNA-Seq) was performed on CL from the late luteal phase and compared with normally luteolyzing CL collected at the prepartum P4 decrease, and following antigestagen (Aglepristone)- treatment. The analysis of transcriptomes presented herein supports the hypothesis describing luteal regression in non-pregnant dogs as a degenerative process devoid of the acute luteolytic principle and without an acute involvement of the immune system observed prepartum. more...

Organism:

Canis lupus familiaris

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20988

15 Samples

Download data: TXT

(Submitter supplied) Despite much investigation, mechanisms conferring stage specific responsiveness of the corpus luteum (CL) to prostaglandin F2 (PG) are unknown. The objective of this study was to identify PG- induced changes in transcriptome of bovine CL specific to d 11 ( PG responsive) but not d 4 (PG refractory) CL associated with luteolysis. CL were collected from heifers at 0, 4 and 24 h following PG injection on d 4 and 11 of the estrous cycle (n = 5 animals/treatment) and isolated RNA labeled and hybridized to Affymetrix GeneChip Bovine Genome Arrays. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

30 Samples

Download data: CEL

(Submitter supplied) In monoovulatory species like bovines, a striking diversity in regulation of the corpus luteum (CL) function exists within species during different stages of the luteal phase. The function and life span of CL appears to be regulated by the interplay of both luteotrophic and luteolytic factors, whose roles and actions are varied from one species to another. Although, studies continue to expand our current understanding of the cellular and molecular actions of prostaglandin F 2alpha (PGF2α - a physiological luteolysin)-induced luteolysis, knowledge of the mechanism whereby, PGF2α mediates its luteolytic actions remains poorly understood. more...

Organism:

Bubalus bubalis; Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

12 Samples

Download data: CEL

(Submitter supplied) Luteolysis of the corpus luteum (CL) during non-fertile cycles involves a cessation of progesterone (P4) synthesis (functional regression) and subsequent structural remodeling. The molecular processes responsible for initiation of luteal regression in the primate CL are poorly defined. Therefore, a genomic approach was utilized to systematically identify differentially expressed genes in the rhesus macaque CL during spontaneous luteolysis. more...

Organism:

Macaca mulatta

Type:

Expression profiling by array

Platform:

GPL3535

12 Samples

Download data: CEL, CHP

(Submitter supplied) This study was designed to provide a genome-wide analysis of the effects of luteinizing hormone (LH) ablation/replacement versus steroid ablation/replacement on gene expression in the developed corpus luteum (CL) in primates during the menstrual cycle. Naturally cycling, female rhesus monkeys were left untreated (Control; n = 4) or received one of the following treatments for three days beginning on Day 9 of the luteal phase: daily injection of the gonadotropin-releasing hormone (GnRH) antagonist (Antide; n = 5), Antide + recombinant human LH (A+LH; n = 4), Antide + LH + the 3b-HSD antagonist Trilostane (A+LH+TRL; n = 4), and Antide + LH + TRL + progesterone replacement with a synthetic progestin R5020 (A+LH+TRL+ R5020; n = 5). more...

Organism:

Macaca mulatta

Type:

Expression profiling by array

Platform:

GPL3535

22 Samples

Download data: CEL, CHP

(Submitter supplied) To explore chorionic gonadotropin (CG)-regulated gene expression in the primate corpus luteum (CL), adult female rhesus macaques were treated with a model of simulated early pregnancy (SEP). Total RNA was isolated from individual CL and hybridized to Affymetrix™ GeneChip Rhesus Macaque Genome Arrays The level of 1192 transcripts changed expression > 2-fold (one-way ANOVA, FDR correction; P<0.05) during SEP when compared to Day 10 untreated controls, and the majority of changes occurred between Days 10 and 12 of SEP. more...

Organism:

Macaca mulatta

Type:

Expression profiling by array

Platform:

GPL3535

20 Samples

Download data: CEL, CHP

(Submitter supplied) The LH-like molecule chorionic gonadotropin (CG) is secreted by the macaque conceptus during and following implantation, “rescuing” the CL from impending regression and extending its functional lifespan in early pregnancy for approximately two weeks. CG binds to the same receptor as LH; i.e., LHCGR, and promotes production of steroids and other factors such as relaxin (RLN1). Our research group recently used Affymetrix™ rhesus macaque total genome arrays to compare the effects of CG on the luteal transcriptome from rhesus females during simulated early pregnancy (SEP) with changes during luteal regression in the non-fecund menstrual cycle. more...

Organism:

Macaca mulatta

Type:

Expression profiling by array

Platform:

GPL3535

33 Samples

Download data: CEL, CHP

(Submitter supplied) The molecular and cellular processes required for development, function, and regression of the primate corpus luteum (CL) are poorly defined. We hypothesized that there are dynamic changes in gene expression occurring during the CL lifespan, which represent proteins and pathways critical to its regulation. Therefore, a genomic approach was utilized to systematically identify differentially expressed genes in the rhesus macaque CL during the luteal phase of natural menstrual cycles. more...

Organism:

Macaca mulatta

Type:

Expression profiling by array

Platform:

GPL3535

20 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens; Bos taurus

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL23055 GPL23507

32 Samples

Download data: RCC

(Submitter supplied) Although rescue of the corpus luteum is required for pregnancy, luteal function during maternal recognition of pregnancy remains largely unexplored. CL were collected from pregnant cattle on days 14, 17, 20, and 23, to encompass the maternal recognition of pregnancy period. Next Generation Sequencing was used to profile mRNA abundance during this time, while tandem mass spectrometry and Nanostring technology were used to profile proteins and miRNA, respectively. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23055

16 Samples

Download data: XLSX

(Submitter supplied) Although rescue of the corpus luteum is required for pregnancy, luteal function during maternal recognition of pregnancy remains largely unexplored. CL were collected from pregnant cattle on days 14, 17, 20, and 23, to encompass the maternal recognition of pregnancy period. Nanostring technology was used to profile miRNA. A total of 27 miRNA changed. MiRNA that increased were predicted to inhibit phosphatidylinositol signaling, while those that decreased may be negative regulators of steroidogenesis. more...

Organism:

Homo sapiens; Bos taurus

Type:

Other

Platform:

GPL23507

16 Samples

Download data: RCC, XLSX

(Submitter supplied) The corpus luteum (CL) is essential for maintenance of pregnancy in all mammals and luteal rescue, which occurs around day 16-19 in the cow, is necessary to maintain luteal progesterone production. Transcriptomic and proteomic profiling were performed to compare the day 17 bovine CL of the estrous cycle and pregnancy. Among mRNA and proteins measured, 140 differentially abundant mRNA and 24 differentially abundant proteins were identified. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15749

8 Samples

Download data: XLSX

(Submitter supplied) The downregulated pathways in early pregnant CL unraveled in this study span a wide range of luteal functions: angiogenesis, apoptosis and survival, steroidogenesis, and ECM remodeling. Notably, selective DEGs in luteinized granulosa cells (LGCs) were modulated by IFNT in vitro in a similar manner to their regulation in the CL of P cows, suggesting that IFNT may contribute to the characteristics of early pregnant CL.

Organism:

Bos indicus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29504

12 Samples

Download data: XLSX

(Submitter supplied) Next-generation sequencing (RNA-Seq) was performed on canine placenta Placental samples collected at natural prepartum luteolysis were compared with those samples obtained from dogs at mid-pregnancy stage. Furthermore, in order to better understand the involvement of P4 and PGR-dependent downstream regulatory mechanisms during initiation of parturition in the dog, samples derived from dogs in which prepartum luteolysis/abortion was induced with the antigestagen aglepristone at mid-pregnancy, were included. more...

Organism:

Canis lupus familiaris

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24229

9 Samples

Download data: TXT