GEO DataSets

Analysis of U937 cells transfected with GFP-(PML-RAR)α (PR) or GFP-PR2VR (cleavage-resistant mutant) and GFP-sorted at 6 and 9 hrs. Neutrophil elastase (NE) cleaves PR, the fusion protein that initiates acute promyelocytic leukemia. Results provide insight into leukemogenicity of NE-resistant PR.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 genotype/variation, 2 time sets

Platform:

GPL570

Series:

GSE21550

12 Samples

Download data: CEL

(Submitter supplied) Previous studies in our laboratory demonstrated that the azurophil granule protease neutrophil elastase (NE) cleaves PML-RARA (PR), the fusion protein that initiates acute promyelocytic leukemia (APL). Further, NE deficiency reduces the penetrance of APL in a murine model of this disease. We therefore predicted that NE-mediated PR cleavage might be important for its ability to initiate APL. To test this hypothesis, we generated a mouse expressing NE-resistant PR. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4172

Platform:

GPL570

12 Samples

Download data: CEL

(Submitter supplied) RARA haploinsufficiency is an invariable consequence of t(15;17) reciprocal translocations in acute promyelocytic leukemia (APL). Furthermore, retinoids and RARA activity have been implicated in hematopoietic self-renewal, lineage commitment and neutrophil maturation. We and others therefore predicted that RARA haploinsufficiency would contribute to APL pathogenesis. To test this hypothesis we crossed RARA+/- mice with mice expressing PML-RARA from the Cathepsin G locus (mCG-PR). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6193

10 Samples

Download data: CEL

(Submitter supplied) To identify the top 20 up-regulated genes in NB4 TRIB3 shRNA cells in comparison with NB4 control shRNA cells, we examined the microarray gene expression profile of these groups above. Despite the fact that combined therapy of all-trans retinoic acid (ATRA) with arsenic trioxide (ATO) or chemotherapy dramatically improves the prognosis of patients with acute promyelocytic leukemia (APL), these regimens can cause systemic infections and secondary leukemias. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL18943

6 Samples

Download data: PAIR

(Submitter supplied) To identify the top 20 up-regulated genes in CD34+ cells from AML patients in comparison with healthy donors, we examined the microarray gene expression profile of CD34+ blasts from patients with newly diagnosed AML vs CD34+ normal cells from healthy donors. Despite the fact that combined therapy of all-trans retinoic acid (ATRA) with arsenic trioxide (ATO) or chemotherapy dramatically improves the prognosis of patients with APL, these regimens can cause systemic infections and secondary leukemias. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL15207

6 Samples

Download data: CEL