GEO DataSets

Temporal analysis of HEK293 cells stably-expressing wildtype NOD2 receptor or variant NOD2L1007fsinsC and treated with muramyl dipeptide (MDP). MDP-induced NOD2 activation induces inflammatory and antibacterial factors. Results provide insight into molecular basis of NOD2-mediated immune response.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 3 genotype/variation, 3 time sets

Platform:

GPL570

Series:

GSE22611

27 Samples

Download data: CEL

(Submitter supplied) NOD2 is an intracellular receptor for the bacterial cell wall component muramyl dipeptide (MDP) and variants of NOD2 are associated with chronic inflammatory diseases of barrier organs e.g. Crohn disease, asthma and atopic eczema. It is known that activation of NOD2 induces a variety of inflammatory and antibacterial factors. The exact transcriptomal signatures that define the cellular programs downstream of NOD2 activation and the influence of the Crohn-associated variant L1007fsinsC are yet to be defined. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4416

Platform:

GPL570

27 Samples

Download data: CEL

(Submitter supplied) Employing microarray assays, a total of 267 genes were identified that were significantly up- or downregulated in PBMCs of WT-NOD2 patients, compared to healthy donors after challenge with vitamin D (+/-D) and/or a combination (+/-LP) of LPS (lipopolysaccharide) and PGN (peptidoglycan) (p < 0.05; threshold: ≥ 2-fold change). For further analysis by real-time PCR, 12 genes with known impact on inflammation and immunity were selected that fulfilled predefined expression criteria. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL23159

24 Samples

Download data: CEL, CHP

(Submitter supplied) Short title: Innate Gene/miRNA MDP-response in CD RNA-seq and small RNA-seq were used to identify the expression of gene and microRNA (miRNA) in monocytes from healthy controls and patients with Crohn's Disease (CD), with and without NOD2 (nucleotide-binding oligomerization domain-containing protein 2) single nucleotide polymorphisms (SNPs) after stimulation with muramyl dipeptide (MDP).

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL10999

28 Samples

Download data: BW

(Submitter supplied) microRNA expression in human monocyte-derived DCs following stimulation with NOD2 ligand MDP, TLR2 ligand Pam3CSK4, or both.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL10850

32 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of human monocyte-derived dendritic cells (MDDCs), comparing cells transfected with miR-29a mimic, or miR-29b mimic, or negative control.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

6 Samples

Download data: TXT

(Submitter supplied) Nod2 has been extensively characterized as a bacterial sensor that induces an antimicrobial and inflammatory gene expression program. Therefore, it is unclear why Nod2 mutations that disrupt bacterial recognition are paradoxically among the highest risk factors for Crohn’s disease, which involves an exaggerated immune response directed at intestinal bacteria. Previous studies from our lab have shown that mice deficient in Atg16L1, another Crohns disease susceptibility gene, develop abnormalities in Paneth cells, specialized epithelial cells in the small intestine involved in antimicrobial responses. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

6 Samples

Download data: CEL, TXT