GEO DataSets

Analysis of calyx of Held globular bushy cells at different maturation stages: P3, P8 and P21. Calyx of Held presynaptic terminals in the auditory brainstem develop into axosomatic synapses. Results provide insight into mechanisms underlying development of mammalian synaptic structure and function.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 3 cell type, 3 development stage sets

Platform:

GPL1355

Series:

GSE49599

20 Samples

Download data: CEL

(Submitter supplied) Calyx of Held giant presynaptic terminals in the medial nucleus of the trapezoid body of the auditory brainstem form axosomatic synapses that have advanced to one of the best-studied synaptic system of the mammalian brain. As the auditory system matures and adjusts to high fidelity synaptic transmission, the calyx undergoes extensive structural and functional changes: it is formed around postnatal day 3 (P3), achieves immature function until hearing onset around P10 and can be considered mature from P21 onwards. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS5257

Platform:

GPL1355

20 Samples

Download data: CEL

(Submitter supplied) The transcriptional events accompanying synaptogenesis are largely unknown, or have been studied in systems in which synapse formation occurs gradually over time. With a system in which synaptogenesis is synchronized and controllable, molecular or biochemical techniques can be used to examine cellular events across cultures on a wide scale, as synapses develop. Here, we have triggered synaptogenesis in immunopurified subplate neurons by coculturing with cortical feeder layers and have used microarrays to investigate the transcriptional events occuring in this more defined and controllable system. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL1355

20 Samples

Download data: CEL, TXT

(Submitter supplied) The superior olivary complex (SOC) is an essential auditory brainstem structure involved in sound localization. In order to identify the genetic program underlying its maturation, we profiled the transcriptome of the rat SOC at postnatal days (P)0, P4, P16, and P25, using genome-wide microarrays (41,012 sequences). Differences in gene expression between two consecutive stages were highest between P4 versus P16 (3.6%), but dropped to 0.06% between P16 versus P25. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL7294

42 Samples

Download data: TXT

(Submitter supplied) The superior olivary complex (SOC) is a prominent auditory brainstem center and represents one of the prime model systems to study the development of sensory circuits. We recently performed genome-wide microarray studies to analyze the SOC-related genetic program at postnatal day (P)4 and P25. Here, we extended this analysis by including two additional time points, P0 and P16. Data sets were validated by qRT-PCR. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL7294

36 Samples

Download data: TXT

(Submitter supplied) To investigate the participation of deafness genes in the genetic program of the superior olivary complex (SOC), a prominent auditory brainstem center, we performed a comparative genome-wide microarray based gene expression analysis of the rat SOC and the rat brain at postnatal days (P) P4 and P25. Data were validated by qRT-PCR. Statistical analyses revealed 912 oligos up-regulated in the SOC at P4 and 1609 at P25. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL7294

28 Samples

Download data: TXT

(Submitter supplied) After inactivation of Hoxa5 at postnatal days (P)1-P4, we established RNA-seq profiling with RNA extracted from P21 brainstem of tamoxifen-treated Hoxa5flox/flox;CMV-CreERT2+/- (Hoxa5 cKO) pups and tamoxifen-treated Hoxa5flox/flox;CMV-CreERT2-/-(Hoxa5 control) pups

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

6 Samples

Download data: FPKM_TRACKING, GTF, TXT