GEO DataSets

(Submitter supplied) Our two main aims were 1) to isolate age-related changes in gene expression in queens of the eusocial bumble bee, Bombus terrestris; and 2) to determine whether experimentally increasing the costs of reproduction (by removing eggs) caused changes in age-related gene expression in these queens. To address these aims we extracted RNA from three key tissues (brain, fat body and ovary) from queens at two time points (10% and 60% mortality phases). more...

Organism:

Bombus terrestris

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30019

70 Samples

Download data: CSV

(Submitter supplied) Our aim was to identify the genes that are responsible for caste differentiation in the primitively eusocial bumble bee, Bombus terrestris. To do this we extracted RNA from both queen- and worker-destined larvae. We extracted RNA from three key stages during bumble bee development (before, during, and after their caste becomes fixed), and then sent the sequencing to the Earlham Institute. They used mRNAseq to isolate the RNA from each developmental stage and caste pathway.

Organism:

Bombus terrestris audax

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22726

20 Samples

Download data: CSV

(Submitter supplied) Bumblebees (Hymenoptera: Apidae) are important pollinating insects that play pivotal roles in crop production and natural ecosystem services. To achieve a comprehensive profile of accessible chromatin regions and provide clues for all possible regulatory elements in the bumblebee genome, we did ATAC-seq for Bombus terrestris samples derived from its four developmental stages: egg, larva, pupa, and adult, respectively. more...

Organism:

Bombus terrestris

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL26656

8 Samples

Download data: NARROWPEAK

(Submitter supplied) The switch between biological states during key life-history transitions requires major reprogramming at the behavioural level that is under the control of the brain. In this study we focused on major life-history transitions in bumblebee queens (Bombus terrestris) that involve switching from virgin to mated and reproductively mature. To reveal the molecular processes underpinning the behavioural changes that accompany these transitions, we characterised the neurogenomic state of queens that succeeded or failed in making these transitions. more...

Organism:

Bombus terrestris

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18289

33 Samples

Download data: XLSX

(Submitter supplied) Our aim was to use next-generation sequencing to identify the miRNAs associated with caste determination in queen-destined and worker-destined larvae of the bumble bee, Bombus terrestris. We found that two miRNAs (miR-6001-5p and miR-6001-3p) were upregulated in queen-destined larvae that had passed the critical developmental period when caste becomes irreversible in this species. The two miRNAs form a duplex that is expressed from the gene VHDL, a homologue of Vitellogenin. more...

Organism:

Bombus terrestris

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL19585

8 Samples

Download data: CSV

(Submitter supplied) Here, we examined the transcriptomic changes associated with diapause and CO2 treatment in B. terrestris queens before, during and post diapause and in the short and long term after CO2 treatment

Organism:

Bombus terrestris

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL19585 GPL20914

25 Samples

Download data: GFF, TXT

(Submitter supplied) Our aims in this study were: 1) to identify the miRNAs of the bumble bees Bombus terrestris and B. impatiens; 2) to compare the total numbers of miRNAs between both bumble bee species and between them and the honey bee, Apis mellifera; and 3) to test whether the sequences and expression patterns of miRNAs were conserved between species. To investigate each of these aims we used miRNA-seq (deep sequencing of miRNA-enriched libraries) in B. more...

Organism:

Bombus terrestris

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL19585

8 Samples

Download data: CSV

(Submitter supplied) Expression of bumblebees (Bombus terrestris) from four colonies exposed to 3 different genotypes of the trypanosome parasite Crithidia bombi

Organism:

Bombus terrestris

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18289

16 Samples

Download data: TXT