GEO DataSets

(Submitter supplied) We report the transcriptional response to Colorado potato beetle herbivory in leaves of the highly beetle resistant Solanum chacoense diploid line USDA8380-1 (80-) and a susceptible F2 individual (EE501F2_093) derived from a cross between 80-1 and a beetle susceptible line S. chacoense M6. Sampling tissue in a time course during adult Colorado potato beetle feeding provides novel insight to the transcriptomic defense response to this important pest.

Organism:

Solanum chacoense

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27550

24 Samples

Download data: TXT

(Submitter supplied) Analysis of the mapped reads showed that 496 transcripts are differentially expressed between resistant and susceptible plants at 16-wks post-transplant. Of these, 188 transcripts are down-regulated in resistant plants, as well as significantly enriched on chromosome 2, while up-regulated genes are distributed across the genome. Previously uncharacterized uridine diphosphate-glycosyltransferases and ethylene response transcription factors within the QTL region identified by bulk-segregant analysis are candidate genes for further analysis.

Organism:

Solanum chacoense

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27550

24 Samples

Download data: TXT

(Submitter supplied) Sexual reproduction in flowering plants involves intimate interactions between the growing pollen tube and the female reproductive structure. These interactions start immediately after pollen landing on the stigma and continue during the pollen tube journey through the style and the ovary. Thus, well before fertilization, genes in the gynoecium are affected by the growing pollen tubes. Genes activated at a distance in the ovary before pollen tubes arrival represent one class of such genes. more...

Organism:

Solanum chacoense

Type:

Expression profiling by array

Platform:

GPL10411

102 Samples

Download data: TXT

(Submitter supplied) To provide a broad analysis of gene expression changes in developing embryos from a solanaceous species, we produced amplicon-derived microarrays with 7741 ESTs isolated from Solanum chacoense ovules bearing embryos from all developmental stages. Our aims were to: 1) identify genes expressed in a tissue-specific and temporal-specific manner; 2) define clusters of genes showing similar patterns of spatial and temporal expression; and 3) identify stage-specific or transition-specific candidate genes for further functional genomic analyses.We analyzed gene expression during S. more...

Organism:

Solanum chacoense

Type:

Expression profiling by array

Platform:

GPL10411

62 Samples

Download data: TXT

(Submitter supplied) The Platform data table reflects a condensed representation of the array's duplicate features. Protocol: PCR products were resuspended in 10 µl of 50% DMSO and arrayed from 384 well microtiter plates onto UltraGAPSTM slides (Corning incorporated, Corning, NY, USA). A total of 7741 ESTs spotted in duplicate along with a variety of controls.

Organism:

Solanum chacoense

2 Series

164 Samples

Download data: TXT