GEO DataSets

(Submitter supplied) We report the application of RNA-seq technology for high-throughput profiling of gene transcription in M. tuberculosis H37Ra strains. By obtaining over four billion bases of sequence from mRNA, we generated genome-wide gene transcription maps of wild-type and mpbR-deleted Mycobacterium tuberculosis H37Ra strains. We find that a group of genes is significantly up-regulated in the mpbR-deleted mutant strain. more...

Organism:

Mycobacterium tuberculosis H37Ra

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27144

6 Samples

Download data: TXT

(Submitter supplied) Transcription profile of intracellular H37Ra of bEnd.3 and Raw 264.7 cells 3 d.p.i

Organism:

Mycobacterium tuberculosis H37Ra

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29448

4 Samples

Download data: TXT

(Submitter supplied) Compare the gene expression difference between MRA_2010 knockout strains and wild-type strains by RNA sequencing in Mycobacterium tuberculosis H37Ra. The goal of this study is that detection cmtR acts as a transcriptional factor and regulates the target genes expression.

Organism:

Mycobacterium tuberculosis H37Ra

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27144

6 Samples

Download data: XLSX

(Submitter supplied) Purpose: Understanding the functional genomics of M.tb (Mycobacterium tuberculosis) and development of novel anti-M.tb drugs and vaccines needs an efficient gene edit tool. The aim of this study was to describe an easy and efficient gene edit tool for functional genomics study of M.tb. Method: A plasmid was designed containing mini CRISPR array and 400bp up and downside homologues DNA sequences from the target gene interposed by EGFP or BFP. more...

Organism:

Mycobacterium tuberculosis H37Ra

Type:

Other

Platform:

GPL23674

3 Samples

Download data: VCF

(Submitter supplied) Objectives: Mtb adaptation is the major threat to affective disease control.The objective of this study was to identify major contributors participating in Mtb adaptation process during sub-lethal drug exposure through next generation sequencing Methods: The sequence reads that passed quality filters were analyzed through Tophat-2 and HT-Seq after being unified with housekeeping genes expression level.Adaptation analysis was performed through classifying genes expression in count table by K-Means clustering algorithm and find interested genes pattern Results: Genes responsible for lipid metabolism strongly repressed while involved in virulence and information pathways up regulated under sub-lethal kanamycin stress.Moreover an adaptive network comprise of Ra1750,Ra3160,Ra3161,Ra2492 and Ra3893 identified in kanamycin treated Mtb Conclusions: Mtb has a complex regulatory inter-connected gene network supporting its survival under diverse environmental conditions.

Organism:

Mycobacterium tuberculosis H37Ra

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23674

7 Samples

Download data: TSV

(Submitter supplied) Genome-wide distribution of topoisomerase I and DNA gyrase is directed by transcription induced supercoiling

Organism:

Mycobacterium tuberculosis H37Ra; Mycobacterium tuberculosis H37Rv

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL17967 GPL23183

5 Samples

Download data: BED, TXT

(Submitter supplied) Synchronised cultures of Mycobacterium tuberculosis (M. tb H37Ra DnaAcos115) were used to determine the cell cycle dependent gene expression.

Organism:

Mycobacterium tuberculosis; Mycobacterium tuberculosis H37Ra

Type:

Expression profiling by array

Platform:

GPL4057

18 Samples

Download data: GPR

(Submitter supplied) Cyclic di-GMP (c-di-GMP) is a ubiquitous second messenger that regulates many biological processes in bacteria. The genome in Mycobacterium tuberculosis encodes a single copy of the diguanylate cyclase gene (dgc) responsible for c-di-GMP synthesis. To determine the role of c-di-GMP signaling in M. tuberculosis, the mutant strain of Δdgc was generated in the virulent H37Rv strain. We used whole genome microarray expression profiling as a discovery platform to identify the genes controlled by c-di-GMP in M. more...

Organism:

Mycobacterium tuberculosis; Mycobacterium tuberculosis H37Ra

Type:

Expression profiling by array

Platform:

GPL15565

5 Samples

Download data: TXT

(Submitter supplied) To uncover mutations underlying the attentuation of H37Ra, we embarked on sequencing its genome using a novel massive parallel pyrosequencing technology. Comparison with the virulent H37Rv parental strain revealed only few polymorphisms, one of which was located in the DNA binding domain of the transcriptional regulator PhoP. Keywords: Expresion profiling

Organism:

Mycobacterium tuberculosis H37Ra; Mycobacterium tuberculosis; Mycobacterium tuberculosis H37Rv

Type:

Expression profiling by array

Platform:

GPL5095

50 Samples

Download data: TXT

(Submitter supplied) see manufacturer's website

Organism:

Mycobacterium tuberculosis H37Ra

Download data: XLSX

(Submitter supplied) See manufacturer's web site at http://www.agilent.com/

Organism:

Mycobacterium tuberculosis H37Ra

1 Series

5 Samples

Download data

(Submitter supplied) See manufacturer's web site at http://www.agilent.com/

Organism:

Mycobacterium tuberculosis H37Ra

Download data