GEO DataSets

(Submitter supplied) Brugia malayi is a parasitic nematode that causes lymphatic filariasis in humans. A total of 178 novel microRNA were identified from short read transcriptional data, which when combined with known Brugia microRNAs yielded a total of 284 microRNA. Of these, 123 microRNA sequences (43%) are differentially expressed over the mammalian life stages of B. malayi that we examined. Putative targets of these microRNA were identified from inversely expressed target clusters that contain valid seed sequences for the corresponding microRNAs. more...

Organism:

Brugia malayi; Wolbachia endosymbiont of Brugia malayi

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL28005

21 Samples

Download data: GTF, TSV

(Submitter supplied) Lymphatic filarial nematodes maintain a mutualistic association with the intracellular bacterium Wolbachia. Wolbachia populations expand following infection of the mammalian host, to support larval growth and development. Utilizing transcriptomic data from Brugia malayi over the first two weeks post-infection, we present an analysis of the biochemical pathways that are involved in Wolbachia population growth and regulation in support of larval development. more...

Organism:

Brugia malayi; Wolbachia endosymbiont strain TRS of Brugia malayi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26792

12 Samples

Download data: TXT

(Submitter supplied) We report our microarray analysis of Brugia malayi microfilariae-derived miRNA comparing parasite-derived EVs and supernatants Microarray analysis was performed using isolated RNA from three biological replicates of Brugia malayi microfilariae with a focus on the parasite-derived EVs and supernatant

Organism:

synthetic construct; Brugia malayi

Type:

Non-coding RNA profiling by array

Platform:

GPL19117

9 Samples

Download data: CEL

(Submitter supplied) Brugia malayi, Wolbachia (wBm), and Aedes aegypti transcriptome reads from across the B. malayi FR3 life cycle

Organism:

Brugia malayi; Aedes aegypti; Wolbachia endosymbiont of Brugia malayi

Type:

Expression profiling by high throughput sequencing

5 related Platforms

81 Samples

Download data: TXT

(Submitter supplied) To understand the genes regulating the molting of L3 to L4 larvae upon induction with ascorbic acid over a 10-day period in a controlled in-vitro molting model of the filarial parasite B.malayi.

Organism:

Brugia malayi

Type:

Expression profiling by array

Platform:

GPL8207

8 Samples

Download data: TXT

(Submitter supplied) Lymphatic filariasis (LF) threatens nearly 20% of the world’s population and has handicapped one-third of the 120 million people currently infected. Current control and eventual elimination of LF rely on mass drug administration (MDA) programs with three drugs: ivermectin (IVM), albendazole (ALB), and diethylcarbamazine (DEC). Only the mechanism of action of albendazole is well-understood. . To gain a better insight into antifilarial drug action, we treated gerbils with patent B. more...

Organism:

Brugia malayi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22883

68 Samples

Download data: TXT

(Submitter supplied) We report the application of Cappable-seq to selectively enrich prokaryotic endosymbiont transcripts from mixed host-symbiont total RNA.

Organism:

Brugia malayi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22883

4 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Brugia malayi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18547

34 Samples

Download data

(Submitter supplied) In this study, we analyzed transcriptomic profiles from ivermectin-treated and -untreated B. malayi adult females using Next Generation RNA Sequencing technology at different concentrations (100 nM, 300 nM and 1 µM) and time points (24, 48, 72 h, and 5 days). Our analysis revealed altered expression of multiple genes involved in meiosis, as well as oxidative phosphorylation which were significantly down-regulated as early as 24 hours post-exposure. more...

Organism:

Brugia malayi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18547

20 Samples

Download data: TXT

(Submitter supplied) In this study, we analyzed transcriptomic profiles from ivermectin-treated and -untreated B. malayi adult females using Next Generation RNA Sequencing technology at different concentrations (100 nM, 300 nM and 1 µM) and time points (24, 48, 72 h, and 5 days). Our analysis revealed altered expression of multiple genes involved in meiosis, as well as oxidative phosphorylation which were significantly down-regulated as early as 24 hours post-exposure. more...

Organism:

Brugia malayi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18547

14 Samples

Download data: TXT

(Submitter supplied) We examined the global transcriptomic profile of B. malayi females at four different time points (6 biological replicates per time point) over 5 days in culture. Focusing on genes that are differentially expressed at various time points, we observed a general perturbation of the expression profile between dissection from the host and receipt after shipment. The expression of a few genes remained changed at the end of the experiment, after 5 days under controlled conditions; in particular, genes encoding cuticle collagens were prominently represented and strongly overexpressed.

Organism:

Brugia malayi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18547

24 Samples

Download data: TXT

(Submitter supplied) Comparative analysis of gene expression profiles provided novel insights into the genes that are transcriptionally active in infective and developing larvae of two closely related species. Species differences may indicate different metabolic adaptations that could affect host specificity, tissue tropism, and pathogenicity

Organism:

Wolbachia endosymbiont strain TRS of Brugia malayi; Brugia malayi; Onchocerca volvulus; Wuchereria bancrofti; Brugia pahangi

Type:

Expression profiling by array

Platform:

GPL10232

24 Samples

Download data: TXT

(Submitter supplied) Comparison of small RNA profiles across nematode evolution

Organism:

Romanomermis culicivorax; Nippostrongylus brasiliensis; Hypsibius dujardini; Brugia malayi; Globodera pallida; Pristionchus pacificus; Enoplus brevis; Paragordius varius; Caenorhabditis elegans; Trichinella spiralis; Odontophora rectangula

Type:

Non-coding RNA profiling by high throughput sequencing

11 related Platforms

22 Samples

Download data: FA, FASTA

(Submitter supplied) Doxycycline treatment affects gene expression in Wolbachia and Brugia malayi adult female worms in vivo

Organism:

Brugia malayi; Onchocerca volvulus; Wuchereria bancrofti; Wolbachia endosymbiont strain TRS of Brugia malayi

Type:

Expression profiling by array

Platform:

GPL10232

8 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of adult males and females of the lymphatic dwelling filarial parasite Brugia malayi Keywords: Gender based transcripts, filaria

Organism:

Brugia malayi

Type:

Expression profiling by array

Platform:

GPL7391

3 Samples

Download data: TXT

(Submitter supplied) The filarial nematodes Brugia malayi, Wuchereria bancrofti and Onchocerca volvulus cause elephantiasis, dermatitis and blindness, resulting in severe morbidity in developing countries. 1.3 billion people are at risk of infection. Targeting the essential Wolbachia endobacteria of filarial nematodes with doxycycline has proven to be an effective therapy, resulting in a block in embryogenesis and worm development, and macrofilaricidal effects. more...

Organism:

Brugia malayi; Onchocerca volvulus; Litomosoides sigmodontis; Wolbachia endosymbiont strain TRS of Brugia malayi; Wuchereria bancrofti

Type:

Expression profiling by array

Platform:

GPL10232

6 Samples

Download data: CSV, TXT

(Submitter supplied) Filarial nematodes are arthropod-borne nematodes that cause a variety of economically important diseases such as onchocerciasis (river blindness), lymphatic filariasis, and heartworm disease. The most widespread filarial disease of humans is lymphatic filariasis, caused by worms in the genera Wuchereria and Brugia. Lymphatic filariasis is an economic and social burden in endemic countries and affects approximately 119 million people worldwide (Michael, 1997). more...

Organism:

Brugia malayi; Brugia pahangi

Type:

Expression profiling by array

Platform:

GPL8207

10 Samples

Download data: CSV

(Submitter supplied) Filarial nematodes are arthropod-borne nematodes that cause a variety of economically important diseases such as onchocerciasis (river blindness), lymphatic filariasis, and heartworm disease. The most widespread filarial disease of humans is lymphatic filariasis, caused by worms in the genera Wuchereria and Brugia. Lymphatic filariasis is an economic and social burden in endemic countries and affects approximately 119 million people worldwide (Michael, 1997). more...

Organism:

Brugia malayi

Type:

Expression profiling by array

Platform:

GPL8207

4 Samples

Download data: CSV, TXT

(Submitter supplied) Filarial nematodes are arthropod-borne nematodes that cause a variety of economically important diseases such as onchocerciasis (river blindness), lymphatic filariasis, and heartworm disease. The most widespread filarial disease of humans is lymphatic filariasis, caused by worms in the genera Wuchereria and Brugia. Lymphatic filariasis is an economic and social burden in endemic countries and affects approximately 119 million people worldwide (Michael, 1997). more...

Organism:

Brugia malayi; Brugia pahangi

Type:

Expression profiling by array

Platform:

GPL8207

6 Samples

Download data: CSV, TXT

(Submitter supplied) Microarray technology permits high throughput comparisons of gene expression in different parasite stages or sexes. We now report the first use of this technology for analysis of gene expression in filarial worms. The slide array (comprised of 65 mer oligos representing 3569 EST clusters) was spotted with sequences selected from the extensive Brugia malayi EST database (http://nema.cap.ed.ac.uk/nematodeESTs/nembase.html#Annotation). more...

Organism:

Brugia malayi

Type:

Expression profiling by array

Platform:

GPL1483

8 Samples

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