GEO DataSets

(Submitter supplied) a high-throughput Illumina/Solexa sequencing was conducted, 478262, 702593, 835394 and 894277 unique sRNAs was discovered from four periods of M. robertsii infection, uninfected (0h), infected for 12h, 24h and 36h. Then, 7, 7, 6 and 7 known pre-miRNAs were obtained, and 33, 58, 54 and 52 candidate novel miRNAs was detected in four periods. Further analysis showed that 24 of those candidate novel miRNAs were matched to other known insect miRNAs, while 36 of those miRNAs lacked sequence homologues of insect organisms.

Organism:

Locusta migratoria

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL23516

4 Samples

Download data: TXT

(Submitter supplied) The human transcription factor (TF) CGGBP1 (“CGG Binding Protein”) is conserved only in amniotes, and is believed to derive from the zf-BED and Hermes transposase DNA-binding domains (DBDs) of a hAT DNA transposon. Here, we examine the DNA binding preferences of a wide variety of metazoan CGGBP1-like TFs with this bipartite domain using PBM experiments. The derived motifs are available at ...

Organism:

Petromyzon marinus; Acipenser ruthenus; Gallus gallus; Erpetoichthys calabaricus; Rhizophagus irregularis; Paramormyrops kingsleyae; Acyrthosiphon pisum; Salmo salar; Fundulus heteroclitus; Sparus aurata; Chrysemys picta; Austrofundulus limnaeus; Anabas testudineus; Amphiprion percula; Salarias fasciatus; Aedes aegypti; Culex quinquefasciatus; Latimeria chalumnae; Cyprinus carpio; synthetic construct; Hucho hucho; Amphiprion ocellaris; Labeo rohita; Mola mola; Sphaeramia orbicularis; Anabarilius grahami; Locusta migratoria; Crocodylus porosus; Homo sapiens; Rhodnius prolixus; Rhipicephalus appendiculatus; Kryptolebias marmoratus; Nematostella vectensis; Acanthochromis polyacanthus; Hippocampus comes; Hofstenia miamia

Type:

Other

Platform:

GPL11260

258 Samples

Download data: TXT

(Submitter supplied) Using hight throughput sequencing and data processing, we identified miRNA and quantified their expression in the fat body of adult females of Locusta migratoria.

Organism:

Locusta migratoria

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL25868

12 Samples

Download data: XLSX

(Submitter supplied) The transcriptome and DGE analysis of the fat body and ovary of L. migratoria based on the Illumina short-read sequencing technology and De novo assembly. Research on the trade-offs between immunity and reproduction is contributing significantly to the understanding of the fitness of organisms in nature.

Organism:

Locusta migratoria

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18662

12 Samples

Download data: FASTA, TXT

(Submitter supplied) The migratory locust, Locusta migratoria, is a serious agricultural pest and important insect model to study insect digestion and feeding behavior. The gut is one of the primary interfaces between the insect and its environment. Nevertheless, knowledge on the gut transcriptome of L. migratoria is still very limited. With the development of two EST databases from L. migratoria (whole body and central nervous system (CNS)) and one EST database from Schistocerca gregaria (CNS), an abundance of transcript data was made available for locusts. more...

Organism:

Locusta migratoria; Schistocerca gregaria

Type:

Expression profiling by array

Platform:

GPL22105

2 Samples

Download data: GPR

(Submitter supplied) Protease inhibitors (PIs) are among the most ubiquitous plant defences against herbivorous insects. These inhibitors arrest proteolytic digestion by binding to the proteases in the gut. However, many pest insects have coevolved mechanisms to counteract these effects. While the nature of these counter-defensive strategies has been well described for several insect pests, our understanding of how these responses are triggered and regulated remains limited. more...

Organism:

Locusta migratoria; Schistocerca gregaria

Type:

Expression profiling by array

Platform:

GPL11172

8 Samples

Download data: GPR

(Submitter supplied) Background: Responses to hypoxia have been investigated in many species; however, comparative study between conspecific geographical populations in different altitude regions is rare, especially for invertebrates . The migratory locust, Locusta migratoria, is widely distributed both on high-altitude Tibetan Plateau (TP) and on low-altitude North China Plain (NP). TP locusts have inhabited Tibetan Plateau since Quaternary glaciations events and thus probably have evolved superior capacity to deal with hypoxia. more...

Organism:

Locusta migratoria

Type:

Expression profiling by array

Platform:

GPL14920

18 Samples

Download data: GPR

(Submitter supplied) Background: Hypoxia can affect aerobic organisms profoundly. Biological responses to extreme hypoxia have been well studied. However, it is not well characterized how living organisms respond to mild hypoxia, and how they distinguish different levels of hypoxia. Results: We examined the transcriptional responses of locusts using microarrays to reveal their strategies to cope with mild hypoxia. Mitochondrial activities were systemically suppressed, mainly involving energy production and mitochondrial biogenesis. more...

Organism:

Locusta migratoria

Type:

Expression profiling by array

Platform:

GPL14920

6 Samples

Download data: GPR

(Submitter supplied) Desert locusts (Schistocerca gregaria) show an extreme form of phenotypic plasticity and can transform between a cryptic solitarious phase and a swarming gregarious phase. The two phases differ extensively in behavior, morphology and physiology but very little is known about the molecular basis of these differences. We used our recently generated Expressed Sequence Tag (EST) database derived from S. more...

Organism:

Locusta migratoria; Schistocerca gregaria

Type:

Expression profiling by array

Platform:

GPL11172

16 Samples

Download data: GPR

(Submitter supplied) We report the application of single-molecule-based sequencing technology for high-throughput profiling of nervous system in locust Locusta migratoria manilensis. By obtaining over 57,000,000 bases of sequence from central nervous system, we generated 101836 contigs and 69440 scaffolds. We finally get 41179 unigene with an average length of 570bp. There are 5519 unigenes beyond the length of 1000bp. Using BLAST searches of the NR, NT, Swiss-Prot, KEGG and COG databases we are able to identify 13552 unigene (E<0.0001). Comprehensive assessment of all the unigenes by comparing with the studied genes of other insects nervous system reveals that our unigene are broadly representative of the transcriptome of insect nervous system. Our data provides the most large-scale EST-project for locust nervous system, which greatly benefits the exploring of this insect. In addition, we identify a large number of novel nervous genes which can be used in systematic studies of locust and other insects.

Organism:

Locusta migratoria manilensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11245

1 Sample

Download data: FA

(Submitter supplied) All the reports on insect small RNAs come from holometabolous insects. However, small RNAs of hemimetabolous insects have not yet been investigated.Study of hemimetabolous insect small RNAs could provide more insights into evolution and function of small RNAs in hemi- and holometabolous insects. The locust is an important, economically harmful hemimetabolous insect and its phase changes is an interesting phenomenon.Here, we used high-throughput sequencing to characterize and compare the small RNA transcriptomes of gregarious and solitary phases in locusts. more...

Organism:

Locusta migratoria

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL9308

2 Samples

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(Submitter supplied) Agilent-034971 Locusta-Schisto-totalMA-110617 EST-based microarrays: 1. A set of unique Locusta ESTs (from LocustDB and the CNS database), 2. Schistocerca ESTs having no homolog in the unique Locusta set. The microarray platform contains probes representing (i) all EST sequences retrieved from LocustDB [1], (ii) all EST sequences retrieved from the L. migratoria central nervous system deep-sequencing project [2] that did not appear to be represented in LocustDB and (iii) all S. more...

Organism:

Locusta migratoria; Schistocerca gregaria

1 Series

2 Samples

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(Submitter supplied) Oligos are arrayed in Greiner 384-well flat-bottom plates. Each well contains 600 pmol of 70-mer oligo. Resuspend oligos in water to 20 uM and rearray 5 µL into 384-well, Genetix polystyrene V-bottom plates (cat# X6004). Allow Genetix plates to dry through passive water evaporation in a protected environment (e.g., chemical hood). Before printing, add 5 µL of 1X Printing Buffer to each well. This can be done the night before a print run is started. more...

Organism:

Locusta migratoria

2 Series

24 Samples

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(Submitter supplied) We recently developed an 'expressed sequence tag' (EST) database derived from the desert locust (Schistocerca gregaria) central nervous system [Badisco L, Huybrechts J, Simonet G, Verlinden H, Marchal E et al. (2011) Transcriptome analysis of the desert locust central nervous system: production and annotation of a Schistocerca gregaria EST database. PLoS One 6: e17274.]. The microarray platform contains probes corresponding to all these S. more...

Organism:

Locusta migratoria; Schistocerca gregaria

2 Series

24 Samples

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