GEO DataSets

(Submitter supplied) In this study we fed groups of female house flies one of two Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli) or control broth and used RNA-seq to determine differential gene expression at 4 hours post-feeding. Flies that ingested P. aeruginosa induced expression of genes encoding digestive proteins in the gut but did not induce AMPs. Flies in all treatment groups constitutively expressed some paralogs of lysozyme and AMP genes at the transcript level.

Organism:

Musca domestica

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30537

28 Samples

Download data: CSV

(Submitter supplied) To investigate how MdCht9 contribute to the development and digestion of Musca domestica, we silenced the chitinase gene 9 (MdCht9) by its dsRNA, while silenced the GFP as the control. Then we compared the differental expression genes between the test group and the control group.

Organism:

Musca domestica

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30537

6 Samples

Download data: XLS

(Submitter supplied) Purpose: House fly has a stable polygenic sex determination system. The male determining factor (Mdmd) is commonly found on the Y chromosome (Y^M) or the third chromosome (III^M). These proto-Y chromosomes are clinally distributed, with Y^M found most commonly in northern latitudes and III^M most commonly found at southern latitudes, hinting at possible genotype-by-temperature interactions that maintain the polymorphism. more...

Organism:

Musca domestica

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22576

24 Samples

Download data: CSV

(Submitter supplied) We injected double-stranded RNA (dsRNA) into house fly embryos to knock down transformer (Md-tra), creating sex-reversed males that do not carry a Y chromosome. We also injected dsRNA trageting GFP as a sham treatment. We used RNA-seq to compare gene expression in the sex-reversed males with genotypic (normal) females (injected with dsRNA targeting GFP) and two types of genotypic (normal) males (injected with dsRNA targeting Md-tra or GFP). more...

Organism:

Musca domestica

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22576

24 Samples

Download data: TSV

(Submitter supplied) We performed RNA-seq experiments to examine the effects of house fly proto-Y chromosomes on gene expression. Two different Y^M chromosomes were investigated, and two different III^M chromosomes were investigated. One of the Y^M genotypes also was also heterozygous for third chromosomes that do not carry a male-determining locus to evaluate the effect of third chromosomes on gene expression independent of being a proto-Y.

Organism:

Musca domestica

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22576

29 Samples

Download data: TSV

(Submitter supplied) Hytrosaviridae family members replicate in the salivary glands (SGs) of their adult dipteran hosts and are transmitted to uninfected hosts via saliva during feeding. Despite inducing similar gross symptoms (SG hypertrophy; SGH), hytrosaviruses (SGHVs) have distinct pathobiologies, including sex-ratio distortions in tsetse flies and refusal of infected housefly females to copulate. Via unknown mechanism(s), SGHV replication in other tissues results in reduced fecundity in tsetse flies and total shutdown of vitellogenesis and sterility in housefly females. more...

Organism:

Musca domestica

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22576

6 Samples

Download data: TXT

(Submitter supplied) Using mRNA next generation sequencing, we analyze the transcriptome of Musca domestica embryos through five stages of early development: from syncytial blastoderm to dorsal closure stage.

Organism:

Musca domestica

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15805

10 Samples

Download data: TXT

(Submitter supplied) The 791spin is the spinosad-selected strain derived from 791a, a laboratory strain derived from a multi-resistant field-collected sample of houseflies. The 791a strain proved highly resistant to pyrethroids and some anticholinesterases and showed some resistance to the chitin synthesis-disrupting larvicides.In order to understand the evolution of insecticide resistance, de novo assembly of a spinosad resistant housefly strain 791spin using 454 technology was initiated

Organism:

Musca domestica

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19777

8 Samples

Download data: FASTA, TXT

(Submitter supplied) Sex determination evolves rapidly, often because of turnover of the genes at the top of the pathway. The house fly, Musca domestica, has a multifactorial sex determination system, allowing us to identify the selective forces responsible for the evolutionary turnover of sex determination in action. There is a male determining factor, M, on the Y chromosome (Y^M), which is probably the ancestral state. more...

Organism:

Musca domestica

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19912

16 Samples

Download data: TSV

(Submitter supplied) Illumina HiSeq 2000 RNASeq was conducted on the mRNA fraction of three adult female Musca domestica strains. Two strains were the insecticide susceptible strains CS and aabys, which has five distinct recessive morphological markers, one on each of the five autosomes in M. domestica. The third strain was ALHF, which is a highly insecticide resistant strain obtained in Alabama. The reference transcriptome was generated de novo using Trinity. more...

Organism:

Musca domestica

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15805

4 Samples

Download data: TXT