GEO help: Mouse over screen elements for information.
Status
Public on Feb 18, 2005
Title
Fundulus_192gene_metabolic_array
Technology type
spotted DNA/cDNA
Distribution
non-commercial
Organism
Fundulus heteroclitus
Description
Microarrays were printed using 192 cDNAs from a F. heteroclitus cardiac library encoding essential proteins for cellular metabolism. These cDNAs were a subset of over 33,000 expressed sequences in our database, which all may be found at http://genomics.rsmas.miami.edu/funnybase/super_craw3/ (Oleksiak et al. 2001). These 192 cDNAs were amplified with amine-linked primers and printed on 3-D Link Activated slides (Surmodics Inc., Eden Prairie, MN) using a SpotArray Enterprise piezoelectric microarray printer (PerkinElmer Life Sciences Inc., Boston, MA, USA) at Louisiana State University. Slides were blocked following slide manufacturer protocols. The suite of 192 amplified cDNAs was printed as a group in spatially separated replicates (6 replicates for 178 genes, and 4 replicates for 14 genes). Four hybridization zones of these six replicate arrays were printed per slide, with each zone set separated by a hydrophobic barrier. Keywords = Fundulus Keywords = metabolism Keywords = populations Keywords = tissue-specific gene expression
Web link
http://genomics.rsmas.miami.edu/funnybase/super_craw3/
Contributor(s)
Whitehead A , Crawford DL
Submission date
May 08, 2004
Last update date
May 26, 2005
Contact name
andrew whitehead
E-mail(s)
awhitehead@rsmas.miami.edu
Phone
305-361-4627
Organization name
University of Miami, RSMAS
Department
Street address
City
Miami
State/province
FL
ZIP/Postal code
33149
Country
USA
Samples (108)
GSM25187 , GSM25188 , GSM25189 , GSM25190 , GSM25191 , GSM25192
GSM25193 ,
GSM25194 ,
GSM25195 ,
GSM25196 ,
GSM25197 ,
GSM25198 ,
GSM25199 ,
GSM25200 ,
GSM25201 ,
GSM25202 ,
GSM25203 ,
GSM25204 ,
GSM25205 ,
GSM25206 ,
GSM25207 ,
GSM25208 ,
GSM25209 ,
GSM25210 ,
GSM25211 ,
GSM25212 ,
GSM25213 ,
GSM25214 ,
GSM25215 ,
GSM25216 ,
GSM25217 ,
GSM25218 ,
GSM25219 ,
GSM25220 ,
GSM25221 ,
GSM25222 ,
GSM25223 ,
GSM25224 ,
GSM25225 ,
GSM25226 ,
GSM25227 ,
GSM25228 ,
GSM25229 ,
GSM25230 ,
GSM25231 ,
GSM25232 ,
GSM25233 ,
GSM25234 ,
GSM25235 ,
GSM25236 ,
GSM25237 ,
GSM25238 ,
GSM25239 ,
GSM25240 ,
GSM25241 ,
GSM25242 ,
GSM25243 ,
GSM25244 ,
GSM25245 ,
GSM25246 ,
GSM25247 ,
GSM25248 ,
GSM25249 ,
GSM25250 ,
GSM25251 ,
GSM25252 ,
GSM25253 ,
GSM25254 ,
GSM25255 ,
GSM25256 ,
GSM25257 ,
GSM25258 ,
GSM25259 ,
GSM25260 ,
GSM25261 ,
GSM25262 ,
GSM25263 ,
GSM25264 ,
GSM25265 ,
GSM25266 ,
GSM25267 ,
GSM25268 ,
GSM25269 ,
GSM25270 ,
GSM25271 ,
GSM25272 ,
GSM25273 ,
GSM25274 ,
GSM25275 ,
GSM25276 ,
GSM25277 ,
GSM25278 ,
GSM25279 ,
GSM25280 ,
GSM25281 ,
GSM25282 ,
GSM25283 ,
GSM25284 ,
GSM25285 ,
GSM25286 ,
GSM25287 ,
GSM25288 ,
GSM25289 ,
GSM25290 ,
GSM25291 ,
GSM25292 ,
GSM25293 ,
GSM25294
Series (1)
GSE2293
Variation in tissue-specific gene expression among natural populations of Fundulus heteroclitus
Data table header descriptions
ID
Gene Name
gene name as identified from SwissProt
Shorter Names
shortened gene names as used in manuscript
E.C. #
enzyme commission number
Pathway
pathway number associated with pathway name
Pathway Name
biochemical pathway
GB_ACC
GenBank accession number;
SPOT_ID
Data table
ID
Gene Name
Shorter Names
E.C. #
Pathway
Pathway Name
GB_ACC
SPOT_ID
A1_1
cytochrome c oxidase subunit IV isoform 1
cyto_C_oxi_IV_1
1.9.3.1
14
Oxidative phosphorylation
CN985075
A1_2
cytochrome c oxidase subunit IV isoform 1
cyto_C_oxi_IV_1
1.9.3.1
14
Oxidative phosphorylation
CN985075
A1_3
cytochrome c oxidase subunit IV isoform 1
cyto_C_oxi_IV_1
1.9.3.1
14
Oxidative phosphorylation
CN985075
A1_4
cytochrome c oxidase subunit IV isoform 1
cyto_C_oxi_IV_1
1.9.3.1
14
Oxidative phosphorylation
CN985075
A1_5
cytochrome c oxidase subunit IV isoform 1
cyto_C_oxi_IV_1
1.9.3.1
14
Oxidative phosphorylation
CN985075
A1_6
cytochrome c oxidase subunit IV isoform 1
cyto_C_oxi_IV_1
1.9.3.1
14
Oxidative phosphorylation
CN985075
A10_1
ATP synthase F0 subunit 6 ATPase 6
ATP_syn_F0_6
3.6.1.34
12
Oxidative phosphorylation
CN985062
A10_2
ATP synthase F0 subunit 6 ATPase 6
ATP_syn_F0_6
3.6.1.34
12
Oxidative phosphorylation
CN985062
A10_3
ATP synthase F0 subunit 6 ATPase 6
ATP_syn_F0_6
3.6.1.34
12
Oxidative phosphorylation
CN985062
A10_4
ATP synthase F0 subunit 6 ATPase 6
ATP_syn_F0_6
3.6.1.34
12
Oxidative phosphorylation
CN985062
A10_5
ATP synthase F0 subunit 6 ATPase 6
ATP_syn_F0_6
3.6.1.34
12
Oxidative phosphorylation
CN985062
A10_6
ATP synthase F0 subunit 6 ATPase 6
ATP_syn_F0_6
3.6.1.34
12
Oxidative phosphorylation
CN985062
A11_1
VACUOLAR ATP SYNTHASE SUBUNIT H (V-ATPASE H SUBUNIT) (VACUOLAR PROTON PUMP H SUBUNIT) (V-ATPASE M9.2 SUBUNIT) (V-ATPASE 9.2 KDA MEMBRANE ACCESSORY PROTEIN)
ATP_syn_H_vacuolar
3.6.1.34
12
Oxidative phosphorylation
CN985064
A11_2
VACUOLAR ATP SYNTHASE SUBUNIT H (V-ATPASE H SUBUNIT) (VACUOLAR PROTON PUMP H SUBUNIT) (V-ATPASE M9.2 SUBUNIT) (V-ATPASE 9.2 KDA MEMBRANE ACCESSORY PROTEIN)
ATP_syn_H_vacuolar
3.6.1.34
12
Oxidative phosphorylation
CN985064
A11_3
VACUOLAR ATP SYNTHASE SUBUNIT H (V-ATPASE H SUBUNIT) (VACUOLAR PROTON PUMP H SUBUNIT) (V-ATPASE M9.2 SUBUNIT) (V-ATPASE 9.2 KDA MEMBRANE ACCESSORY PROTEIN)
ATP_syn_H_vacuolar
3.6.1.34
12
Oxidative phosphorylation
CN985064
A11_4
VACUOLAR ATP SYNTHASE SUBUNIT H (V-ATPASE H SUBUNIT) (VACUOLAR PROTON PUMP H SUBUNIT) (V-ATPASE M9.2 SUBUNIT) (V-ATPASE 9.2 KDA MEMBRANE ACCESSORY PROTEIN)
ATP_syn_H_vacuolar
3.6.1.34
12
Oxidative phosphorylation
CN985064
A11_5
VACUOLAR ATP SYNTHASE SUBUNIT H (V-ATPASE H SUBUNIT) (VACUOLAR PROTON PUMP H SUBUNIT) (V-ATPASE M9.2 SUBUNIT) (V-ATPASE 9.2 KDA MEMBRANE ACCESSORY PROTEIN)
ATP_syn_H_vacuolar
3.6.1.34
12
Oxidative phosphorylation
CN985064
A11_6
VACUOLAR ATP SYNTHASE SUBUNIT H (V-ATPASE H SUBUNIT) (VACUOLAR PROTON PUMP H SUBUNIT) (V-ATPASE M9.2 SUBUNIT) (V-ATPASE 9.2 KDA MEMBRANE ACCESSORY PROTEIN)
ATP_syn_H_vacuolar
3.6.1.34
12
Oxidative phosphorylation
CN985064
A12_1
ASPARTATE AMINOTRANSFERASE MITOCHONDRIAL PRECURSOR (TRANSAMINASE A) (GLUTAMATE OXALOACETATE TRANSAMINASE-2)
aspartate_aminotransferaseE_mito
2.6.1.1
20
Glutamate
CN952528
A12_2
ASPARTATE AMINOTRANSFERASE MITOCHONDRIAL PRECURSOR (TRANSAMINASE A) (GLUTAMATE OXALOACETATE TRANSAMINASE-2)
aspartate_aminotransferaseE_mito
2.6.1.1
20
Glutamate
CN952528
Total number of rows: 1124 Table truncated, full table size 129 Kbytes .
Supplementary data files not provided