|
| Status |
Public on Nov 10, 2008 |
| Title |
Chromatin Immunoprecipitation microarray data from antiHA IP in stable LNCaP cells expressing either YFP or HA-SOX4/YFP |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
SOX4 is a critical developmental transcription factor in vertebrates and is required for precise differentiation and proliferation in multiple tissues. In addition, SOX4 is overexpressed in many human malignancies, but the precise role of SOX4 in cancer progression is not well understood. Here we have identified the direct transcriptional targets of SOX4 using a genome-wide localization ChIP-chip analysis.
Keywords: ChIP-chip
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| Overall design |
LNCaP prostate cancer cells were stably infected with a lentivirus containing either HA-SOX4-IRES-YFP or IRES-YFP alone. Cells were sorted for YFP expression and expanded in tissue culture before chromatin immunoprecipitation against the HA epitope tag was performed.
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| |
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| Contributor(s) |
Moreno CS, Scharer C |
| Citation(s) |
19147588 |
| |
| Submission date |
Jun 24, 2008 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Chris Scharer |
| E-mail(s) |
cdschar@emory.edu
|
| Organization name |
Emory University
|
| Department |
Microbiology and Immunology
|
| Lab |
Chris Scharer
|
| Street address |
1510 Clifton Rd, Suite 3086A
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL6325 |
NimbleGen Homo sapiens HG18 promoter 1 of 2 |
| GPL6326 |
NimbleGen Homo sapiens HG18 promoter 2 of 2 |
|
| Samples (10)
|
| GSM299800 |
HA-SOX4 IP Replicate 1 hybridized to array 1 of 2 |
| GSM299801 |
HA-SOX4 IP Replicate 1 hybridized to array 2 of 2 |
| GSM299802 |
HA-SOX4 IP Replicate 2 hybridized to array 1 of 2 |
| GSM299803 |
HA-SOX4 IP Replicate 2 hybridized to array 2 of 2 |
| GSM299804 |
HA-SOX4 IP Replicate 3 hybridized to array 1 of 2 |
| GSM299805 |
HA-SOX4 IP Replicate 3 hybridized to array 2 of 2 |
| GSM299806 |
YFP Control IP Replicate 1 hybridized to array 1 of 2 |
| GSM299807 |
YFP Control IP Replicate 1 hybridized to array 2 of 2 |
| GSM299808 |
YFP Control IP Replicate 2 hybridized to array 1 of 2 |
| GSM299809 |
YFP Control IP Replicate 2 hybridized to array 2 of 2 |
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| This SubSeries is part of SuperSeries: |
| GSE11915 |
SOX4 effect on LNCaP prostate cancer cells |
|
| Relations |
| BioProject |
PRJNA109103 |
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