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| Status |
Public on Oct 01, 2019 |
| Title |
Detection of cryptic unstable transcripts associated with ribosomes in yeast |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Other
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| Summary |
We investigated the association of cryptic unstable transcripts (CUTs) to ribosomes in Saccharomyces cerevisiae. We used 5’cap-sequence followed by sucrose gradient fractionation of polyribosome fractions after ultracentrifugation to assess the relative association of transcripts to the ribosomes. We used 5PSeq approach, which measures ribosome dynamics by sequencing the presence of co-translation mRNA degradation intermediates, to assess if cryptic transcripts are engaged in active translation. We performed 5PSeq after glucose depletion to increase the number of ribosomes stop at the 5’UTR and start codon.
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| Overall design |
We performed 2 biologically independent experiments for polyribosome fractionation and for 5PSeq.
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| Contributor(s) |
Zhang Y, Wang J, Wei W, Steinmetz LM, Pelechano V |
| Citation(s) |
31740578 |
| Submission date |
Aug 28, 2018 |
| Last update date |
Jan 02, 2020 |
| Contact name |
Vicent Pelechano |
| E-mail(s) |
vicente.pelechano.garcia@ki.se
|
| Organization name |
ScilifeLab - Karolinska Institutet
|
| Department |
MTC
|
| Street address |
Nobels väg 16
|
| City |
Solna |
| ZIP/Postal code |
SE-17177 |
| Country |
Sweden |
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| Platforms (2) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
| GPL19756 |
Illumina NextSeq 500 (Saccharomyces cerevisiae) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA488250 |
| SRA |
SRP159016 |
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