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| Status |
Public on Mar 09, 2021 |
| Title |
CASB: A concanavalin A-based sample barcoding strategy for single-cell sequencing [scRNA-seq] |
| Organisms |
Drosophila melanogaster; Homo sapiens; Canis lupus familiaris; Cricetulus griseus; Mus musculus; Rattus norvegicus; Chlorocebus sabaeus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We reported a Concanavalin A-based Barcoding Strategy (CASB) for single-cell and single-nucleus sample multiplexing, which could be followed by different single-cell sequencing techniques. The method involves minimal sample processing, thereby preserving intact transcriptomic or epigenomic patterns. Besides sample multiplexing, the CASB could further improve data quality through doublet identification.
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| |
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| Overall design |
scRNA-seq of a mixture of 16 celltypes from 7 species
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| |
|
| Contributor(s) |
Fang L, Li G, Chen W |
| Citation(s) |
33821571 |
| |
| Submission date |
Jan 18, 2021 |
| Last update date |
Apr 20, 2021 |
| Contact name |
Guipeng Li |
| E-mail(s) |
guipeng.lee@gmail.com, ligp@sustech.edu.cn
|
| Organization name |
Southern University of Science and Technology
|
| Street address |
1088 Xueyuan Avenue
|
| City |
Shenzhen |
| ZIP/Postal code |
518055 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL29623 |
Illumina NovaSeq 6000 (Canis lupus familiaris; Chlorocebus sabaeus; Cricetulus griseus; Drosophila melanogaster; Homo sapiens; Mus musculus; Rattus norvegicus) |
|
| Samples (2) |
| GSM5025076 |
scRNA-seq of a mixture of 16 celltypes from 7 species |
| GSM5025077 |
CASB library for scRNA-seq of a mixture of 16 celltypes from 7 species |
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| This SubSeries is part of SuperSeries: |
| GSE153116 |
CASB: A concanavalin A-based sample barcoding strategy for single-cell sequencing |
|
| Relations |
| BioProject |
PRJNA693032 |
| SRA |
SRP302252 |
