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Series GSE219108 Query DataSets for GSE219108
Status Public on Jun 07, 2023
Title Digital Spatial Profiling of immuno-oncology proteins in mouse hepatocellular carcinoma xenograft treated with THIO-based combination immune therapie
Organism Mus musculus
Experiment type Other
Summary THIO (6-thio-dG) is a nucleoside analog that induces telomeric DNA damage and triggers activation of anti-tumor immunity in several cancer types such as lung and colorectal cancers. To evaluate its effect in hepatocellular carcinoma (HCC), THIO in combination with the current standard care first-line therapy for advanced HCC, anti-PD-L1 and anti-analyze, was tested in immunocompetent mice subcutaneously implanted with syngeneic HCC cell line (Hep55.1c). Formalin-fixed paraffin-embedded (FFPE) tumor tissues were collected after the treatment, and therapeutic modulations of the tumor immune landscape were assessed with Digital Spatial Profiling of 38 immuno-oncology-related proteins in region of interest (ROI) and intra-ROI segments enriched for T cells, other leukocytes, and HCC/stromal cells.
 
Overall design Immunocompetent black 4 mice were subcutaneously implanted with a syngeneic mouse HCC cell line (Hep55.1c). After tumor nodules were formed, mice were treated with vehicle control, THIO, anti-PD-L1 + anti-VEGF, or anti-PD-L1 + anti-VEGF + THIO (n=1 per group). Mice were sacrificed at the end of the treatment, and FFPE tumor tissue blocks were generated. One representative animal was selected for each treatment group, and subjected to Digital Spatial Profiling of 38 immuno-oncology-related proteins. Immunofluorescent (IF) staining of morphology markers (CD3, CD45, pan-cytokeratin [Pan-CK]) was first performed, and based on the IF and H&E staining, region of interest (ROI) was defined in each tissue section. Within each ROI, segments were subsequently selected based on the IF staining enriched for T cells (CD3+), other leukocytes (CD3-/CD45+), and HCC/stromal cells (CD3-/CD45-/Pan-CK+/-), and abundance of the 38 immuno-oncology-related proteins was determined by using the GeoMx Digital Spatial Profiler (NanoString).
 
Contributor(s) Kubota N, Ogunrinde IM, Shay J, Hoshida Y
Citation(s) 37070671
Submission date Nov 30, 2022
Last update date Jun 07, 2023
Contact name Yujin Hoshida
E-mail(s) Yujin.Hoshida@UTSouthwestern.edu
Organization name University of Texas Southwestern Medical Center
Street address 5323 Harry Hines Blvd
City Dallas
State/province TX
ZIP/Postal code 75390
Country USA
 
Platforms (1)
GPL32902 Mouse immuno-oncology-related proteins for digital spatial profiling
Samples (46)
GSM6771661 THIOxenoHCC_roi19_seg004_Tcell
GSM6771662 THIOxenoHCC_roi19_seg001_Tcell
GSM6771663 THIOxenoHCC_roi19_seg002_Tcell
Relations
BioProject PRJNA907046

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE219108_raw.txt.gz 11.0 Kb (ftp)(http) TXT
Processed data included within Sample table

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