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Series GSE228784

Query DataSets for GSE228784

Status

Public on Apr 30, 2023

Title

RNA seq to determine differential gene expression in pgph-2 oe C.elegans animals and the subset of HLH-30-dependent genes on normal growth and excess glucose conditions.

Organism

Caenorhabditis elegans

Experiment type

Expression profiling by high throughput sequencing

Summary

Metabolic stress caused by excess nutrients accelerates aging. We recently demonstrated that the newly-discovered enzyme glycerol-3-phosphate phosphatase (G3PP; gene Pgp), which operates an evolutionarily conserved glycerol shunt that hydrolyzes glucose-derived glycerol-3-phosphate to glycerol, counters metabolic stress and promotes healthy aging in C. elegans. However, the mechanism whereby G3PP activation extends healthspan and lifespan, particularly under glucotoxicity, remained unknown. We demonstrated that G3PP (PGPH-2) overexpression activates three key longevity factors, AMPK, the TFEB homolog HLH-30, and autophagy, and is an attractive target for age-related metabolic disorders linked to excess nutrients. This transcriptomics analysis was designed to determine genes that are differentially regulated in pgph-2 oe animals in comparison to wild-type animals on normal and excess glucose conditions. To determine the role of HLH-30 in the regulation of gene expression, we also used pgph-2 oe; hlh-30 and hlh-30 mutant animals and found a signature mediated by pgph-2 oe and HLH-30-dependent.

Overall design

Synchronized young adult WT, pgph-2 o/e, pgph-2 o/e; hlh-30 and hlh-30 animals and grown on normal growth medium or plates supplemented with 2% glucose are harvested. Total RNA is extracted using trizol and purified with RNA purification kit and subjected to seq analysis.

Contributor(s)

Possik E, Prentki M

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Submission date

Apr 03, 2023

Last update date

Apr 30, 2023

Contact name

Marcas Agostinelli

E-mail(s)

marcus.agostinelli@gmail.com

Phone

8144047650

Organization name

Novogene

Department

Bioinformatics

Street address

2690 W Avenida Azahar

City

Tucson

State/province

Arizona

ZIP/Postal code

85745

Country

USA

Platforms (1)

Illumina NovaSeq 6000 (Caenorhabditis elegans)

Samples (32)

More...

GSM7136481	Synchronized L4/young adult hlh-30 animals grown on NGM - 1
GSM7136482	Synchronized L4/young adult hlh-30 animals grown on NGM - 2
GSM7136483	Synchronized L4/young adult hlh-30 animals grown on NGM - 3

GSM7136484	Synchronized L4/young adult hlh-30 animals grown on NGM - 4
GSM7136485	Synchronized L4/young adult hlh-30 animals grown on 2% glucose - 1
GSM7136486	Synchronized L4/young adult hlh-30 animals grown on 2% glucose - 2
GSM7136487	Synchronized L4/young adult hlh-30 animals grown on 2% glucose - 3
GSM7136488	Synchronized L4/young adult hlh-30 animals grown on 2% glucose - 4
GSM7136489	Synchronized L4/young adult pgph-2 oe animals grown on NGM - 1
GSM7136490	Synchronized L4/young adult pgph-2 oe animals grown on NGM - 2
GSM7136491	Synchronized L4/young adult pgph-2 oe animals grown on NGM - 3
GSM7136492	Synchronized L4/young adult pgph-2 oe animals grown on NGM - 4
GSM7136493	Synchronized L4/young adult pgph-2 oe animals grown on 2% glucose - 1
GSM7136494	Synchronized L4/young adult pgph-2 oe animals grown on 2% glucose - 2
GSM7136495	Synchronized L4/young adult pgph-2 oe animals grown on 2% glucose - 3
GSM7136496	Synchronized L4/young adult pgph-2 oe animals grown on 2% glucose - 4
GSM7136497	Synchronized L4/young adult pgph-2 oe ; hlh-30 animals grown on NGM - 1
GSM7136498	Synchronized L4/young adult pgph-2 oe ; hlh-30 animals grown on NGM - 2
GSM7136499	Synchronized L4/young adult pgph-2 oe ; hlh-30 animals grown on NGM - 3
GSM7136500	Synchronized L4/young adult pgph-2 oe ; hlh-30 animals grown on NGM - 4
GSM7136501	Synchronized L4/young adult pgph-2 oe ; hlh-30 animals grown on 2% glucose - 1
GSM7136502	Synchronized L4/young adult pgph-2 oe ; hlh-30 animals grown on 2% glucose - 2
GSM7136503	Synchronized L4/young adult pgph-2 oe ; hlh-30 animals grown on 2% glucose - 3
GSM7136504	Synchronized L4/young adult pgph-2 oe ; hlh-30 animals grown on 2% glucose - 4
GSM7136505	Synchronized L4/young adult wt animals grown on NGM - 1
GSM7136506	Synchronized L4/young adult wt animals grown on NGM - 2
GSM7136507	Synchronized L4/young adult wt animals grown on NGM - 3
GSM7136508	Synchronized L4/young adult wt animals grown on NGM - 4
GSM7136509	Synchronized L4/young adult wt animals grown on 2% glucose - 1
GSM7136510	Synchronized L4/young adult wt animals grown on 2% glucose - 2
GSM7136511	Synchronized L4/young adult wt animals grown on 2% glucose - 3
GSM7136512	Synchronized L4/young adult wt animals grown on 2% glucose - 4

Relations

BioProject

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE228784_gene_count.txt.gz	1.6 Mb	(ftp)(http)	TXT
SRA Run Selector
Raw data are available in SRA
Processed data are available on Series record

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