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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 01, 2010 |
| Title |
Hepatic gene expression in transgenic mice with conditional liver-specific expression of the SV40 17kT/LT oncoproteins |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The SV40 large (LT) and small (st) antigens are produced from a single alternatively spliced pre-mRNA, that when co-expressed, transform a variety of cells in vitro and in vivo. However, 17kT, a relatively uncharacterized third protein that is co-linear with LT for the first 131 amino acids, is also produced from the early viral pre-mRNA by removal of an additional intron from the LT transcript. Here we report a line of transgenic mice expressing a liver-specific dox-inducible viral transcript that fails to yield any detectable LT protein, yet produces abundant 17kT. Comparative analysis of livers of transgenic mice expressing either 17kT or LT demonstrates that while 17kT is a potent stimulator of cell proliferation, it is ineffective at inducing liver tumor development, due in part, to the failure of 17kT to effectively induce the expression of growth regulators and reactivate expression of imprinted and developmentally regulated hepatic genes. These studies highlight key functional differences between LT and 17kT in their ability to transform quiescent primary epithelial cells in vivo, and demonstrate how specific functional domains within LT impact cell-specific gene expression to promote oncogenesis.
Keywords: genetic modification, SV40, 17kT, LT, Rb, p53, hepatocellular carcinoma, hepatic hyperplasia, differentiation.
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| Overall design |
Affymetrix mouse genome 430 A and B platform was used, single measurements for wild-type and transgenic animals.
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| Contributor(s) |
Comerford SA, Schultz N, Hammer RE |
| Citation(s) |
23552841 |
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| Submission date |
Jul 06, 2006 |
| Last update date |
Sep 19, 2019 |
| Contact name |
Nikolaus Schultz |
| E-mail(s) |
schultz@cbio.mskcc.org
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| Phone |
646-888-2604
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| Organization name |
Memorial Sloan-Kettering Cancer Center
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| Department |
Computational Biology Center
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| Street address |
1275 York Ave, Box 460
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (2) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
| GPL340 |
[MOE430B] Affymetrix Mouse Expression 430B Array |
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| Samples (6)
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| GSM118628 |
Mouse liver LT ApoErtTA:TRE2-TAg_430B |
| GSM444754 |
Mouse liver 17kT ApoErtTA:TRE2-TAg_430A |
| GSM444755 |
Mouse liver 17kT ApoErtTA:TRE2-TAg_430B |
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| Relations |
| BioProject |
PRJNA96457 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE5242_RAW.tar |
267.8 Mb |
(http)(custom) |
TAR (of CEL, CHP, DAT, EXP, RPT) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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