GEO DataSets

(Submitter supplied) Mycobacterium bovis (M. bovis) and Mycobacterium avium subspecies paratuberculosis (MAP) are important pathogens of cattle, causing bovine tuberculosis and Johne’s disease respectively. M. bovis and MAP infect residential macrophages in the lung and intestines respectively and subvert the macrophage biology to create a survival niche. To investigate this interaction we simultaneously studied the transcriptional response of bovine monocyte-derived macrophages to infection with two strains of M. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11153

80 Samples

Download data: TXT

(Submitter supplied) The placenta is arguably the most anatomically variable organ in mammals. Understanding gene expression patterns among placentas from multiple species can illuminate the molecular basis of placenta function as well as potentially enrich our knowledge of the biology of obstetrical syndromes. We measured the expression profiles of the term placenta of multiple therian mammals using RNA-Seq. We identified multiple genes and transcripts which are differentially expressed across lineages including prolactin, relaxin, and galectins, as well as identifying the non-housekeeping genes which are core to placenta function (such as components of annexin complexes) in all species studied.

Organism:

Dasypus novemcinctus; Loxodonta africana; Bos taurus; Pan paniscus; Canis lupus familiaris; Monodelphis domestica; Ateles fusciceps; Mus musculus

Type:

Expression profiling by high throughput sequencing

8 related Platforms

8 Samples

Download data: FASTA, FPKM_TRACKING

(Submitter supplied) Rift Valley Fever Virus (RVFV), a negative-stranded RNA virus, is the etiological agent of the vector-borne zoonotic disease, Rift Valley Fever (RVF). In both humans and livestock, protective immunity can be achieved through vaccination. Earlier and more recent vaccine trials in cattle and sheep demonstrated a strong neutralizing antibody and total IgG response induced by the RVFV vaccine, MP-12. From previous work, protective immunity in sheep and cattle vaccinates normally occurs from 7 to 21 days after inoculation with MP-12. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11153

45 Samples

Download data: TXT

(Submitter supplied) MicroRNAs (miRNAs) are short non-coding RNAs that post-transcriptionally regulate expression of mRNAs in many biological pathways. Here we report comprehensive miRNAs profiles by next-gen deep sequencing in Angus cattle divergently selected for residual feed intake (RFI) and identify miRNAs related to feed efficiency in beef cattle Results: Two microRNA libraries were constructed from pooled RNA extracted from livers of low and high RFI cattle, and sequenced by Illumina genome analyser. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL11153

2 Samples

Download data: TXT

(Submitter supplied) RNA sequencing (RNA-Seq) was performed on rumen papillae from 16 steers with variation in gain and feed intake.

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11153

16 Samples

Download data: TXT

(Submitter supplied) Puberty is a complex physiological event measured by various indicator traits in genetic improvement programs of beef cattle; thus, developing a more complete understanding of the genes and regulatory pathways and networks involved in puberty will provide knowledge to help improve genetic selection strategies. Herein, we characterized the transcriptome of five reproductive tissues (i.e. hypothalamus, pituitary gland, ovary, uterus, and endometrium) as well as tissues known to be relevant to growth and metabolism needed for cattle to achieve puberty (i.e., longissimus dorsi muscle, fat, and liver). more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL11153 GPL15749

61 Samples

Download data: XLSX

(Submitter supplied) The estrous cycles of Limousin heifers (n = 30) were synchronized by insertion of a controlled internal drug release (CIDR) device (1.94 g progesterone; Pfizer Animal Health) placed into the vagina for 8 days. A 0.5 mg intramuscular injection of a prostaglandin F2α (PG) analogue (PG, Estrumate, Shering-Plough Animal Health, Hertfordshire, UK) was administered 1 day before CIDR removal. Heifers were checked for standing estrus and only those exhibiting estrus (Day 0) were used. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11153

30 Samples

Download data: TXT

(Submitter supplied) All cows experience bacterial contamination and tissue injury in the uterus postpartum, instigating a local inflammatory immune response. However mechanisms that control inflammation and achieve a physiologically functioning endometrium, while avoiding disease in the postpartum cow are not succinctly defined. This study aimed to identify novel candidate genes indicative of inflammation resolution during involution in healthy beef cows. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11153

6 Samples

Download data: TXT

(Submitter supplied) Background: Weaning of beef calves is a necessary husbandry practice and involves separating the calf from its mother, resulting in numerous stressful events including dietary change, social reorganisation and the cessation of the maternal-offspring bond and is often accompanied by housing. While much recent research has focused on the physiological response of the bovine immune system to stress in recent years, little is known about the molecular mechanisms modulating the immune response. more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11153

48 Samples

Download data: TXT

(Submitter supplied) Cellular mechanisms that contribute to low estradiol concentrations produced by the preovulatory ovarian follicle in cattle with a compromised metabolic status (such as lactatino) are largely unknown. To gain insight into the main metabolic mechanisms affecting preovulatory follicle function RNAseq profiling was conducted on non-lactating Holstein-Friesian heifers (n=16) and lactating Holstein-Friesian cows (n=17) at three stages of preovulatory follicle development: A) newly selected dominant follicle in the luteal phase (Selection); B) follicular phase before the LH surge (Differentiation) and C) pre-ovulatory phase after the LH surge (Luteinization). more...

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11153

65 Samples

Download data: TXT

(Submitter supplied) The aim of this study was to identify and quantify microRNAs and other small regulatory RNAs expressed in primary retinal microvascular endothelial cells (RMECs) using deep sequencing. RMECs were isolated, RNA extracted, a small RNA library prepared and deep sequencing performed. A total of 6.8 million reads were mapped to 250 known microRNAs in miRBase (release 16). Several novel microRNAs and multiple new members of the miR-2284/2285 family were detected. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL11153

1 Sample

Download data: TXT

(Submitter supplied) Transcriptomes of bovine IVF embryos showing distinct developmental statuses were sequenced

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11153

2 Samples

Download data: TXT