GEO DataSets

(Submitter supplied) We performed CAGE-Seq on dissected ovaries and testes as well as female and male carcasses of two species of Drosophila (D. melanogaster and D. pseudoobscura). These data are used to map transcription start sites. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf

Organism:

Drosophila melanogaster; Drosophila pseudoobscura

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL17883 GPL11203

8 Samples

Download data: BED

(Submitter supplied) We report the evolutionary behaviour of Polycomb group proteins, their recruitment factors and their underlying sequences by performing ChIP-seq analysis in 4-5 different Drosophila species. We demonstrate an extremely high conservation of Polycomb repressive domains across Drosophila species We validate few cases of PRE divergence that shows that cis-driven PRE evolution is a rare event. We further show that PHO recruitment to Polycomb domains is evolutionarily robust to motif changes and that PRC1 stabilizes binding of its key recruiter

Organism:

Drosophila melanogaster; Drosophila pseudoobscura; Drosophila simulans; Drosophila yakuba; Drosophila virilis

Type:

Genome binding/occupancy profiling by high throughput sequencing

6 related Platforms

70 Samples

Download data: BED, BEDGRAPH, TXT

(Submitter supplied) Purpose: Accurate identification of sex-biased genes requires precise measurement of gene expression levels in gonads. This study is designed to provide such data for various Drosophila species to enhance studies of sex-biased gene expression and evolution across the genus. Methods: Virgin flies were collected and aged 6-10 days before dissecting 2-3 replicates of testes or ovaries. Total RNA was extracted using the Arcturus® PicoPure® kit . more...

Organism:

Drosophila pseudoobscura; Drosophila yakuba; Drosophila ananassae

Type:

Expression profiling by high throughput sequencing

4 related Platforms

13 Samples

Download data: DIFF, TXT

(Submitter supplied) The model organism Encyclopedia of DNA Elements project (modENCODE) has produced a comprehensive annotation of D. melanogaster transcript models based on an enormous amount of high-throughput experimental data. However, some transcribed elements may not be functional, and technical artifacts may lead to erroneous inference of transcription. Inter-species comparison provides confidence to predicted annotation, since transcriptional activity that has been evolutionarily conserved is likely to have an advantageous function. more...

Organism:

Drosophila eugracilis; Drosophila takahashii; Drosophila ficusphila; Drosophila kikkawai; Drosophila bipectinata; Drosophila rhopaloa; Drosophila ananassae; Drosophila simulans; Drosophila virilis; Drosophila elegans; Drosophila biarmipes; Drosophila melanogaster; Drosophila pseudoobscura; Drosophila mojavensis; Drosophila yakuba

Type:

Expression profiling by high throughput sequencing

34 related Platforms

93 Samples

Download data: BAM, BED, RPKM, TXT