GEO DataSets

(Submitter supplied) An incompatibility P-7 plasmid pCAR1 can be efficiently transferred among artificial microcosms in the presence of divalent cations Ca2+ and Mg2+. One on one mating assays between Pseudomonas strains with different plasmids showed that the promotion of transfer efficiency by divalent cations was also found in other plasmids including pB10 and NAH7, whereas the impacts were larger in IncP-7 plasmids. more...

Organism:

Pseudomonas putida KT2440; Pseudomonas; Pseudomonas fluorescens Pf0-1

Type:

Expression profiling by genome tiling array

Platforms:

GPL8298 GPL8296 GPL6591

24 Samples

Download data: BAR, CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Pseudomonas; Pseudomonas putida; Pseudomonas putida KT2440

Type:

Expression profiling by genome tiling array; Genome binding/occupancy profiling by genome tiling array

Platforms:

GPL8296 GPL6591

18 Samples

Download data: BAR, CEL

(Submitter supplied) MvaT proteins are recognized as a member of H-NS family proteins in pseudomonads. IncP-7 conjugative plasmid pCAR1, which was originally found in Pseudomonas resinovorans, carries an mvaT homologous gene, pmr. In Pseudomonas putida KT2440 bearing pCAR1, it was previously reported that pmr and chromosomally-encoded homologous genes, turA and turB, are majorly transcribed, and that Pmr interacts with TurA and TurB in vitro (Yun et al. more...

Organism:

Pseudomonas; Pseudomonas putida

Type:

Expression profiling by genome tiling array; Genome binding/occupancy profiling by genome tiling array

Platform:

GPL6591

9 Samples

Download data: BAR, CEL

(Submitter supplied) To elucidate the impact of the carriage of carbazole-degradative plasmid pCAR1 on biofilm formation by host bacteria, we compared biofilm morphology of pCAR1-free and pCAR1-carrying three Pseudomonas hosts: P. putida KT2440, P. aeruginosa PAO1 and P. fluorescens Pf0-1 using confocal laser scanning microscopy. Although pCAR1 carriage had no significant influence on biofilm formed by PAO1 and Pf0-1, pCAR1-carrying KT2440 became filamentous and formed flat biofilm, whereas pCAR1-free KT2440 formed mushroom-like biofilm. more...

Organism:

Pseudomonas; Pseudomonas putida; Pseudomonas putida KT2440

Type:

Expression profiling by genome tiling array

Platforms:

GPL8296 GPL6591

7 Samples

Download data: BAR, BPMAP, CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Pseudomonas putida KT2440; Pseudomonas; Pseudomonas putida

Type:

Expression profiling by genome tiling array

Platforms:

GPL8296 GPL6591

12 Samples

Download data: BAR, CEL, TXT

(Submitter supplied) Nucleoid-associated proteins (NAPs) are known to fold bacterial DNA and influence global transcription. Incompatibility P-7 plasmid pCAR1 carries three genes encoding NAPs: H-NS family protein Pmr, NdpA-like protein Pnd, and HU-like protein Phu. Because previous reports about plasmid-encoded NAPs mainly focused on H-NS homologs, functions and importance of different kinds of NAPs encoded on a plasmid remained unknown. more...

Organism:

Pseudomonas; Pseudomonas putida

Type:

Expression profiling by genome tiling array

Platform:

GPL6591

6 Samples

Download data: BAR, CEL, TXT

(Submitter supplied) Impacts of plasmid carriage on its host cell were comprehensively analyzed using conjugative plasmid pCAR1 in the three different kinds of hosts, Pseudomonas putida KT2440, P. aeruginosa PAO1, and P. fluorescens Pf0-1. Various analyses of the host phenotype showed that pCAR1 carriage reduced host fitness, swimming motility, and resistances to osmotic- or pH-stress, and brought about the alterations of primary metabolic capacities in the TCA cycle or those several steps away from the TCA cycle in the host cells. more...

Organism:

Pseudomonas aeruginosa; Pseudomonas putida KT2440; Pseudomonas fluorescens Pf0-1; Pseudomonas aeruginosa PAO1; Pseudomonas; Pseudomonas fluorescens; Pseudomonas putida

Type:

Expression profiling by genome tiling array

4 related Platforms

36 Samples

Download data: BAR, CEL

(Submitter supplied) RNA mapping on pCAR1 carried by KT2440, CA10, PAO1, Pf0-1, HS01, and JCM 2778

Organism:

Pseudomonas aeruginosa; Metapseudomonas resinovorans; Pseudomonas fluorescens; Pseudomonas; Pseudomonas putida; Pseudomonas chlororaphis subsp. chlororaphis

Type:

Expression profiling by genome tiling array

Platform:

GPL6591

6 Samples

Download data: BAR, CEL

(Submitter supplied) H-NS family proteins are known as a member of nucleoid associated proteins (NAPs) conserved in genus Pseudomonas bacteria. Incompatibility group (Inc) P-7 archetype plasmid pCAR1 is transmissible among various Pseudomonas strains and carries genes encoding H-NS family protein, Pmr. P. putida KT2440 is one of pCAR1 hosts, which possesses five genes encoding H-NS family proteins, PP_1366 (TurA), PP_3765 (TurB), PP_0017 (TurC), PP_3693 (TurD), and PP_2947 (TurE) on its chromosome. more...

Organism:

Pseudomonas; Pseudomonas putida; Pseudomonas putida KT2440

Type:

Genome binding/occupancy profiling by genome tiling array; Expression profiling by genome tiling array

Platforms:

GPL8296 GPL6591

6 Samples

Download data: BAR, BED, CEL

(Submitter supplied) Plasmid carriage requires appropriate expression of the genes on the plasmid or host chromosome through cooperative transcriptional regulation. To clarify the impact of plasmid carriage on the host chromosome, we compared the chromosomal RNA maps of plasmid-free and plasmid-containing host strains using the incompatibility group P-7 archetype plasmid pCAR1, which is involved in carbazole degradation, and three distinct Pseudomonas strains. more...

Organism:

Pseudomonas putida KT2440; Pseudomonas aeruginosa PAO1; Pseudomonas; Pseudomonas fluorescens Pf0-1

Type:

Expression profiling by genome tiling array

4 related Platforms

9 Samples

Download data: BAR, CEL

(Submitter supplied) High-resolution mapping of the pCAR1 plasmid transcriptomes in the original host Pseudomonas resinovorans CA10 and the transconjugant Pseudomonas putida KT2440(pCAR1) While plasmids are replicated autonomously in their hosts, the transcription of plasmid genes can be switched through horizontal transfer by the change in the transcriptional networks. To examine whether and how the plasmid genome is differentially expressed, we analyzed the transcriptomes of the 199,035-bp IncP-7 carbazole catabolic and conjugative plasmid pCAR1 in the original host Pseudomonas resinovorans CA10 and the transconjugant Pseudomonas putida KT2440(pCAR1) during growth on carbazole or succinate using the high-resolution tiling array. more...

Organism:

Pseudomonas; Pseudomonas putida; Metapseudomonas resinovorans

Type:

Expression profiling by array

Platform:

GPL6591

4 Samples

Download data: BAR, CEL

(Submitter supplied) This is an Affymetrix custom-commercial tiling array that covers the IncP-7 plasmid pCAR1 (RefSeq: NC_004444) at 9-bp density. BPMAP files are provided as supplementary files. pCAR1_8b520435FF-3.bpmap represents the forward strand of pCAR1, and pCAR1_8b520435FR-3.bpmap represents the reverse strand of pCAR1. Protocol: See manufacturer's web site.

Organism:

Pseudomonas

11 Series

54 Samples

Download data: BPMAP, TXT