(Submitter supplied) 1. Oligos are resuspended and adjusted to 10µM in 150mM phosphatebuffer, pH8.5 in Genetix 384-wells plates 2. Array production is done using a Biorobotics MicroGridII spotter located in a low dust cleanroom (ISO7/ downflow ISO5) at 18C, at 48% humidity 3. Slide postprocessing is carried out according to CodeLink protocol: Printed slides are kept at 20C, 75% humidity for 24 hours 4. Residual reactive groups are blocked using pre-warmed blocking solution (50 mM ethanolamine, 0.1 M Tris (pH 9) at 50C for 30 min; slides are rinsed thoroughly with milliQ water; slides are washed with 4x SSC, 0.1% SDS (pre-warmed to 50C) for 30 min at 50C on a shaker 5.
more...- Organism:
- Saccharomyces cerevisiae
- 34 Series
- 4152 Samples
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