GSM127223[Accession] - GEO DataSets

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Items: 3

Select item 2000055011.

Comparison of protein expression profiles in NCI-60 cell panel containing nine difererent cancer tissue types.

(Submitter supplied) Because most potential molecular markers and targets are proteins, proteomic profiling is expected to yield more direct answers to functional and pharmacological questions than does transcriptional profiling. To aid in such studies, we have developed a protocol for making reverse-phase protein lysate microarrays with larger numbers of spots than previously feasible. Our first application of these arrays was to profiling of the 60 human cancer cell lines (NCI-60) used by the National Cancer Institute to screen compounds for anticancer activity. more...

Organism:: Homo sapiens

Type:: Protein profiling by protein array

Platform:: GPL4081
176 Samples

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Accession:: GSE5501

ID:: 200005501

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 1000040812.

NCI-60 cell line Reverse Phase Protein Lysate Array_modified

(Submitter supplied) 640 spots (plus eight spatial registration marks for use in image processing) on a 21 x 35 mm area of nitocellulose membrane. Protocol: Arrays were prepared on nitrocellulose-coated galss slides (FAST Slides, Schleicher & Schuell) by using a pin-in-ring format GMS 417 arrayer (Affymetrix, Santa Clara,CA) with four 500 um diameter pins. Because the cell lysates were viscous, we used pin-in-ring format to avoid clogging of quills. more...